NGHIÊN CỨU ẢNH HƯỞNG MÔI TRƯỜNG NUÔI CẤY LÊN SỰ SINH TRƯỞNG CỦA CÂY GAI MA VƯƠNG (Tribulus terrestris L.) TRONG ĐIỀU KIỆN IN VITRO
Nội dung chính của bài viết
Tóm tắt
Cây Gai ma vương chủ yếu phân bố ở các tỉnh ven biển miền Trung như Quảng Bình, Thừa Thiên Huế, Khánh Hòa, Ninh Thuận. Tuy nhiên, do quá trình khai thác bừa bãi và môi trường sống bị thu hẹp nên loài cây này đang đứng trước nguy cơ tuyệt chủng và đã được đưa vào Sách Đỏ Việt Nam. Nghiên cứu đã đánh giá tác động của các môi trường nuôi cấy khác nhau lên sự sinh trưởng của cây Gai ma vương. Kết quả cho thấy thành phần và nồng độ các chất dinh dưỡng trong môi trường nuôi cấy có ảnh hưởng rõ rệt đến các chỉ tiêu sinh trưởng của cây. Cây Gai ma vương sinh trưởng tốt nhất trong điều kiện nuôi cấy in vitro môi trường giảm giảm ½ NH4NO3, các chỉ tiêu về sinh trưởng đều có kết quả tốt đạt 9,73 lá, chiều cao cây 10,7cm, trọng lượng tươi đạt 501,04mg và khô đạt 53,19mg sau 8 tuần nuôi cấy. Kết quả nghiên cứu cho thấy môi trường nuôi cấy có vai trò quan trọng trong việc thúc đẩy sự sinh trưởng của cây Gai ma vương. Việc lựa chọn thành phần và nồng độ các chất dinh dưỡng phù hợp sẽ giúp tối ưu hóa quá trình nuôi cấy in vitro loài cây này.
Từ khóa
môi trường nuôi cấy, sinh trưởng, in vitro, Gai ma vương
Chi tiết bài viết
Tài liệu tham khảo
Avilla, A., Pereira, M., & Arguello, A. (1998). Nitrogen concentration and proportion of NH4+-N affect potato cultivar response in solid and liquid media. HortScience, 33(2), 336-338.
Ministry of Science and Technology. (2007). Sách Đỏ Việt Nam: Phần II - Thực vật [Vietnam Red Data Book: Part II – Plants]. Science and Technology Publishing House.
Britto, D. T., & Kronzucker, H. J. (2002). NH4+ toxicity in higher plants: a critical review. Journal of plant physiology, 159(6), 567-584. https://doi.org/10.1078/0176-1617-0774
Bui, T. V. (2000). Sinh lí thực vật đại cương [General Plant Physiology]. Education Publishing House.
Capellades, M., Vanderschaeghe, A., Lemeur, R., & Debergh, P. (1990). How important is photosynthesis in micropropagation? In The impact of biotechnology on agriculture: Proceedings of the international conference "The meeting point between fundamental and applied in vitro culture research" (pp. 29-38). Springer Netherlands.
Chang, W. C., & Hsing, Y. I. (1980). In vitro flowering of embryoids derived from mature root callus of ginseng (Panax ginseng). Nature, 284(5754), 341-342. https://doi.org/10.1038/284341a0
Chen, C. (2004). Humidity in plant tissue culture vessels. Biosystems Engineering, 88(2), 231-241.
Cox, W. J., & Reisenauer, H. M. (1973). Growth and ion uptake by wheat supplied nitrogen as nitrate, or ammonium, or both. Plant and soil, 38, 363-380. https://doi.org/10.1007/BF00779019
Duong, C. K. (2003). Nuôi cấy mô thực vật (II) [Plant Tissue Culture (Part II)]. Vietnam National University – Ho Chi Minh Publishing House.
George, E. F., & Davies, W. (2008). Effects of the physical environment. In E. F. George, M. A. Hall, & G.-J. De Klerk (Eds.), Plant propagation by tissue culture (3rd Ed., Vol. 1, pp. 424-468). Springer.
Hegazi, E .S., Mohamed, S. M., El-Sonbaty, M. R., Abd El-Naby, S. K. M., & El-Sharony, T. F. (2011) Effect of potassium nitrate on vegetative growth, nutritional status, yield and fruit quality of olive cv. "Picual". J Hort Sci & Ornamen Plants, 3(3), 252-258.
Jabeen, N., & Ahmad, R. (2011) Foliar application of potassium nitrate affects the growth and nitrate reductase activity in sunflower and safflower leaves under salinity. Not Bot Horti Agrobo. 39(2), 172-178. https://doi.org/10.15835/nbha3926064
Julkiflee, A.L., Uddain, J., & Subramaniam, S. (2014) Efficient micropropagation of Dendrobium Sonia- 28 for rapid PLBs proliferation. Emir J Food Agric, 26(6), 545-551.
Kim, H. J., Lee, J. N., Lim, H. T., & Yeoung, Y. R. (2014) Effect of MS medium strength on the sprouting rate and growth characteristics in meristem culture of strawberry ‘Seolhyang’. Kor J Hort Sci Technol, 32(1), 100-104. https://doi.org/10.7235/hort.2014.13089
Kostenyuk, I., Oh, B. J., & So, I. S. (1999). Induction of early flowering in Cymbidium niveo-marginatum Mak in vitro. Plant Cell Reports, 19, 1-5.
Kozai, T., Kubota, C., & Watanabe, I. (1988). Effects of basal medium composition on the growth of carnation plantlets in auto- and mixotrophic tissue culture. In Symposium on High Technology in Protected Cultivation (Vol. 230, pp. 159-166). International Society for Horticultural Science.
Le, N. T. N., Tran, T. T., Hoang, T. M. N., Vo, N. T. A., Tran, T. T. L., & Quach, V. T. E. (2022). A study on the effects of IBA and BA growth regulators on germination of Tribulus terrestris L. seeds in vitro. Ho Chi Minh City University of Education Journal of Science, 19(12),
1234-1245.
Le, T. L., Nguyen, T. P., Nguyen, H. N. D., & Pham, M. D. N. T. Q. (2015). Effect of NH4NO3 and KNO3 contents on the growth of Lavender plants cultured photoautotrophically. Journal of Biotechnology, 13(4A), 1313-1319.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ngo, T. N. H., Dinh, V. K., & Nguyen, T. Q. (2015). Effect of mineral contents on the growth of Vietnamese ginseng (Panax vietnamensis Ha et grushv.) cultured in vitro under photoautotrophic condition. Journal of Biology, 37(1), 96-102.
Nguyen, L. T. M., Nguyen, T. P. D., Le, T. T. A., & Nguyen, T. Q. (2017). Effects of sucrose concentration, vitamins, light intensity and mineral components on growth of Hibiscus sagittifolius Kurz cultured in vitro. Journal of Biology, 39(1), 86-95.
Pathak, P. S. (1970). Contributions to the ecology of Tribulus terrestris Linn. Agra Univ. J. Res. Sci. 19(2), 149-166.
Pham, M. D., Nguyen, N. H., Hoang, N. N., Nguyen, D. S., & Nguyen, T. Q. (2012). Sự tăng trưởng và tích lũy hợp chất thứ cấp của cây diệp hạ châu đắng nuôi cấy quang tự dưỡng trong điều kiện môi trường giàu CO2.[Growth promotion and secondary metabolite accumulation of Phyllanthus amarus cultured photoautotrophically under carbon dioxide enriched conditio] Journal of Biology, 34(3SE), 249-256.
Preece, J. E., & Sutter, E. G. (1990). Acclimatization of micropropagated plants to the greenhouse and field. In P. C. Debergh & R. H. Zimmerman (Eds.), Micropropagation: Technology and application (pp. 71-93). Kluwer Academic Publishers.
Quach, V. T. E., Thai, L. B. N. T., Tran, T. T., Hoang, T. M. N., & Tran, T. T. L. (2024). A study on the effects of lighting time and cultured medium on germination of Tribulus terrestris L. seeds in vitro. Ho Chi Minh City University of Education Journal of Science, 21(2), 219.
Roland, C. (2020). Tribulus terrestris. The IUCN Red List of Threatened Species 2020.
Rout, G. R., & Das, P. (1994). Somatic embryogenesis and in vitro flowering of 3 species of bamboo. Plant Cell Reports, 13, 683-686. https://doi.org/10.1007/BF00231624
Staikidou, I., Watson, S., Harvey, B. M., & Selby, C. (2005). Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium. Plant Cell, Tissue and Organ Culture, 80, 313-320. https://doi.org/10.1007/s11240-004-1366-0
Stitt, M., & Quick, W. P. (1989). Photosynthetic carbon partitioning: its regulation and possibilities for manipulation. Physiologia plantarum, 77(4), 633-641.
Villamor, C. C. (2010). Influence of media strength and sources of nitrogen on micropropagation of ginger, Zingiber officinale Rosc. E-International Scientific Research Journal, 2(2), 150-155.
Vo, V. C. (2021). Từ điển cây thuốc Việt Nam [Vietnamese medicinal plant dictionary]. Medical Publishing House.
Vu, H. S., Nguyen, T. X., Nguyen, T. H., Duong, T. N. A., Nguyen, M. T., Nguyen, D. P.,
& Phan, T. H. (2020). Study on tuberization of Rehmannia glutinosa (Gaertn.) libosch. Vietnam Journal of Biotechnology, 18(3), 507-516.
Warncke, D. D., & Barber, S. A. (1973). Ammmonium and Nitrate Uptake by Corn (Zea mays L.) as Influenced by Nitrogen Concentration and NH4+/NO3- Ratio 1. Agronomy Journal, 65(6), 950-953. https://doi.org/10.2134/agronj1973.00021962006500060031x
Williams, R. R. (1995). The chemical microenvironment. Kluwer Academic Publishers.
Ziv, M. (1991). Vitrification: Morphological and physiological disorders of in vitro plants. In P. C. Debergh & R. H. Zimmerman (Eds.), Micropropagation: Technology and application (pp. 45-69). Springer.
Ministry of Science and Technology. (2007). Sách Đỏ Việt Nam: Phần II - Thực vật [Vietnam Red Data Book: Part II – Plants]. Science and Technology Publishing House.
Britto, D. T., & Kronzucker, H. J. (2002). NH4+ toxicity in higher plants: a critical review. Journal of plant physiology, 159(6), 567-584. https://doi.org/10.1078/0176-1617-0774
Bui, T. V. (2000). Sinh lí thực vật đại cương [General Plant Physiology]. Education Publishing House.
Capellades, M., Vanderschaeghe, A., Lemeur, R., & Debergh, P. (1990). How important is photosynthesis in micropropagation? In The impact of biotechnology on agriculture: Proceedings of the international conference "The meeting point between fundamental and applied in vitro culture research" (pp. 29-38). Springer Netherlands.
Chang, W. C., & Hsing, Y. I. (1980). In vitro flowering of embryoids derived from mature root callus of ginseng (Panax ginseng). Nature, 284(5754), 341-342. https://doi.org/10.1038/284341a0
Chen, C. (2004). Humidity in plant tissue culture vessels. Biosystems Engineering, 88(2), 231-241.
Cox, W. J., & Reisenauer, H. M. (1973). Growth and ion uptake by wheat supplied nitrogen as nitrate, or ammonium, or both. Plant and soil, 38, 363-380. https://doi.org/10.1007/BF00779019
Duong, C. K. (2003). Nuôi cấy mô thực vật (II) [Plant Tissue Culture (Part II)]. Vietnam National University – Ho Chi Minh Publishing House.
George, E. F., & Davies, W. (2008). Effects of the physical environment. In E. F. George, M. A. Hall, & G.-J. De Klerk (Eds.), Plant propagation by tissue culture (3rd Ed., Vol. 1, pp. 424-468). Springer.
Hegazi, E .S., Mohamed, S. M., El-Sonbaty, M. R., Abd El-Naby, S. K. M., & El-Sharony, T. F. (2011) Effect of potassium nitrate on vegetative growth, nutritional status, yield and fruit quality of olive cv. "Picual". J Hort Sci & Ornamen Plants, 3(3), 252-258.
Jabeen, N., & Ahmad, R. (2011) Foliar application of potassium nitrate affects the growth and nitrate reductase activity in sunflower and safflower leaves under salinity. Not Bot Horti Agrobo. 39(2), 172-178. https://doi.org/10.15835/nbha3926064
Julkiflee, A.L., Uddain, J., & Subramaniam, S. (2014) Efficient micropropagation of Dendrobium Sonia- 28 for rapid PLBs proliferation. Emir J Food Agric, 26(6), 545-551.
Kim, H. J., Lee, J. N., Lim, H. T., & Yeoung, Y. R. (2014) Effect of MS medium strength on the sprouting rate and growth characteristics in meristem culture of strawberry ‘Seolhyang’. Kor J Hort Sci Technol, 32(1), 100-104. https://doi.org/10.7235/hort.2014.13089
Kostenyuk, I., Oh, B. J., & So, I. S. (1999). Induction of early flowering in Cymbidium niveo-marginatum Mak in vitro. Plant Cell Reports, 19, 1-5.
Kozai, T., Kubota, C., & Watanabe, I. (1988). Effects of basal medium composition on the growth of carnation plantlets in auto- and mixotrophic tissue culture. In Symposium on High Technology in Protected Cultivation (Vol. 230, pp. 159-166). International Society for Horticultural Science.
Le, N. T. N., Tran, T. T., Hoang, T. M. N., Vo, N. T. A., Tran, T. T. L., & Quach, V. T. E. (2022). A study on the effects of IBA and BA growth regulators on germination of Tribulus terrestris L. seeds in vitro. Ho Chi Minh City University of Education Journal of Science, 19(12),
1234-1245.
Le, T. L., Nguyen, T. P., Nguyen, H. N. D., & Pham, M. D. N. T. Q. (2015). Effect of NH4NO3 and KNO3 contents on the growth of Lavender plants cultured photoautotrophically. Journal of Biotechnology, 13(4A), 1313-1319.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ngo, T. N. H., Dinh, V. K., & Nguyen, T. Q. (2015). Effect of mineral contents on the growth of Vietnamese ginseng (Panax vietnamensis Ha et grushv.) cultured in vitro under photoautotrophic condition. Journal of Biology, 37(1), 96-102.
Nguyen, L. T. M., Nguyen, T. P. D., Le, T. T. A., & Nguyen, T. Q. (2017). Effects of sucrose concentration, vitamins, light intensity and mineral components on growth of Hibiscus sagittifolius Kurz cultured in vitro. Journal of Biology, 39(1), 86-95.
Pathak, P. S. (1970). Contributions to the ecology of Tribulus terrestris Linn. Agra Univ. J. Res. Sci. 19(2), 149-166.
Pham, M. D., Nguyen, N. H., Hoang, N. N., Nguyen, D. S., & Nguyen, T. Q. (2012). Sự tăng trưởng và tích lũy hợp chất thứ cấp của cây diệp hạ châu đắng nuôi cấy quang tự dưỡng trong điều kiện môi trường giàu CO2.[Growth promotion and secondary metabolite accumulation of Phyllanthus amarus cultured photoautotrophically under carbon dioxide enriched conditio] Journal of Biology, 34(3SE), 249-256.
Preece, J. E., & Sutter, E. G. (1990). Acclimatization of micropropagated plants to the greenhouse and field. In P. C. Debergh & R. H. Zimmerman (Eds.), Micropropagation: Technology and application (pp. 71-93). Kluwer Academic Publishers.
Quach, V. T. E., Thai, L. B. N. T., Tran, T. T., Hoang, T. M. N., & Tran, T. T. L. (2024). A study on the effects of lighting time and cultured medium on germination of Tribulus terrestris L. seeds in vitro. Ho Chi Minh City University of Education Journal of Science, 21(2), 219.
Roland, C. (2020). Tribulus terrestris. The IUCN Red List of Threatened Species 2020.
Rout, G. R., & Das, P. (1994). Somatic embryogenesis and in vitro flowering of 3 species of bamboo. Plant Cell Reports, 13, 683-686. https://doi.org/10.1007/BF00231624
Staikidou, I., Watson, S., Harvey, B. M., & Selby, C. (2005). Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium. Plant Cell, Tissue and Organ Culture, 80, 313-320. https://doi.org/10.1007/s11240-004-1366-0
Stitt, M., & Quick, W. P. (1989). Photosynthetic carbon partitioning: its regulation and possibilities for manipulation. Physiologia plantarum, 77(4), 633-641.
Villamor, C. C. (2010). Influence of media strength and sources of nitrogen on micropropagation of ginger, Zingiber officinale Rosc. E-International Scientific Research Journal, 2(2), 150-155.
Vo, V. C. (2021). Từ điển cây thuốc Việt Nam [Vietnamese medicinal plant dictionary]. Medical Publishing House.
Vu, H. S., Nguyen, T. X., Nguyen, T. H., Duong, T. N. A., Nguyen, M. T., Nguyen, D. P.,
& Phan, T. H. (2020). Study on tuberization of Rehmannia glutinosa (Gaertn.) libosch. Vietnam Journal of Biotechnology, 18(3), 507-516.
Warncke, D. D., & Barber, S. A. (1973). Ammmonium and Nitrate Uptake by Corn (Zea mays L.) as Influenced by Nitrogen Concentration and NH4+/NO3- Ratio 1. Agronomy Journal, 65(6), 950-953. https://doi.org/10.2134/agronj1973.00021962006500060031x
Williams, R. R. (1995). The chemical microenvironment. Kluwer Academic Publishers.
Ziv, M. (1991). Vitrification: Morphological and physiological disorders of in vitro plants. In P. C. Debergh & R. H. Zimmerman (Eds.), Micropropagation: Technology and application (pp. 45-69). Springer.