SỰ HIỆN DIỆN VÀ SỰ ĐỀ KHÁNG KHÁNG SINH CỦA Staphylococcus aureus TRONG MỘT SỐ MẪU THỨC ĂN ĐƯỜNG PHỐ
Nội dung chính của bài viết
Tóm tắt
Thực phẩm đường phố được tiêu thụ rộng rãi do hương vị hấp dẫn, giá cả phải chăng và sự tiện lợi, nhưng có nguy cơ cao nhiễm Staphylococcus aureus, một trong những nguyên nhân hàng đầu gây ngộ độc thực phẩm. Nghiên cứu này nhằm điều tra sự xuất hiện của S. aureus, tình trạng kháng kháng sinh và các gene độc tố của chúng trong 40 mẫu thực phẩm đường phố. Kết quả cho thấy 12,5% mẫu thực phẩm dương tính với S. aureus và 7,5% dương tính với S. aureus kháng methicillin. Tất cả 31/31 chủng phân lập được đều kháng ít nhất một trong các kháng sinh được thử nghiệm, gồm penicillin, gentamicin, cefoxitin, clarithromycin và tetracycline. S. aureus đa kháng thuốc chiếm 45,2% tổng số chủng. Các gene độc tố sea và seb, được phát hiện ở tỉ lệ thấp. Một chủng kí hiệu BTT6, có nguồn gốc từ bánh tráng trộn, được xác định là S. aureus kháng methicillin có mang gene seb. Kết quả cho thấy thực phẩm đường phố tiềm ẩn mối nguy hiểm đối với sức khỏe cộng đồng do tỉ lệ nhiễm S. aureus kháng kháng sinh cao, đặc biệt là S. aureus đa kháng thuốc.
Từ khóa
đa kháng thuốc, đề kháng kháng sinh, MRSA, Staphylococcus aureus, thức ăn đường phố
Chi tiết bài viết
Tài liệu tham khảo
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Staphylococcus aureus isolated from retail raw beef, sheep, and lamb meat in
Turkey. International Journal of Food Microbiology, 361, Article 109461. https://doi.org/10.1016/j.ijfoodmicro.2021.109461
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Article e5520573. https://doi.org/10.1155/2021/5520573
Castro, A., Palhau, C., Cunha, S., Camarinha, S., Silva, J., & Teixeira, P. (2017). Virulence and resistance profile of Staphylococcus aureus isolated from food. Acta Alimentaria, 46(2), Article 2. https://doi.org/10.1556/066.2017.46.2.13
Chajęcka-Wierzchowska, W., Gajewska, J., Wiśniewski, P., & Zadernowska, A. (2020). Enterotoxigenic potential of coagulase-negative staphylococci from ready-to-eat food. Pathogens, 9(9), Article 9. https://doi.org/10.3390/pathogens9090734
CLSI. (2021). M100-Performance Standards for Antimicrobial Susceptibility Testing, 31st Edition. CLSI.
da Silva, A. C., Rodrigues, M. X., & Silva, N. C. C. (2019). Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: A review. Brazilian Journal of Microbiology, 51(1), 347-356. https://doi.org/10.1007/s42770-019-00168-1
Heo, S., Lee, J.-H., & Jeong, D.-W. (2020). Food-derived coagulase-negative Staphylococcus as starter cultures for fermented foods. Food Science and Biotechnology, 29(8), 1023-1035. https://doi.org/10.1007/s10068-020-00789-5
Huynh, V. B., Vuong, T. V., Huynh, T. T. T., Dang, X. S., Huynh, V. T., Tran, T. L., Nguyen, T. T. N., Huynh, B., C., & Nguyen, V. H. (2022). Factors associated with food safety compliance among street food vendors in Can Tho city, Vietnam: Implications for intervention activity design and implementation. BMC Public Health, 22, Article 94. https://doi.org/10.1186/s12889-022-12497-2
Jia, K., Fang, T., Wang, X., Liu, Y., Sun, W., Wang, Y., Ding, T., Wang, J., Li, C., Xu, D., Qiu, J., Liu, Q., & Dong, Q. (2020). Antibiotic resistance patterns of Staphylococcus aureus isolates from retail foods in mainland China: A meta-analysis. Foodborne Pathogens and Disease, 17(5), Article 5. https://doi.org/10.1089/fpd.2019.2686
Krakauer, T. (2019). Staphylococcal Superantigens: Pyrogenic Toxins Induce Toxic Shock. Toxins, 11(3), 178. https://doi.org/10.3390/toxins11030178
Lim, K. L., Khor, W. C., Ong, K. H., Timothy, L., & Aung, K. T. (2023). Occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in Staphylococcus aureus in food and food contact surfaces in Singapore. Microorganisms, 11(7), Article 7. https://doi.org/10.3390/microorganisms11071785
Mejía, F., Castro-del Campo, N., García, A., Rodríguez, K., Cornejo, H., Ahumada-Ruiz, S., Soto-Beltrán, M., Castillo, M., Querol-Audi, J., Chaidez-Quiroz, C., & Martínez-Torres, A. O. (2021). Prevalence and characterization of antibiotic-resistant Staphylococcus aureus recovered from pasteurized cheese commercialized in Panama city markets. Journal of Food Quality, 2021, e9923855. https://doi.org/10.1155/2021/9923855
Nguyen, D., P., Dang, T., L., Le, T., M. H., Tran, T., T., Nguyen, T. N. B., & Quach, K., N. (2020). Prevalence of classical Staphylococcal enterotoxin genes of Staphylococcus aureus isolated from ready-to-eat food in Ho Chi Minh City, Vietnam. Vietnamese Journal of Food Control, 3(4), 9-15. https://doi.org/10.47866/2615-9252/vjfc.1755
Pervin, R., Rahman, M. A., Zereen, F., Ahmed, R., & Alam, M. R. (2019). Isolation, identification and sharacterization of Staphylococcus aureus from raw milk in different places of Savar, Bangladesh. International Journal of Sciences: Basic and Applied Research (IJSBAR), 48(7), Article 7.
Rodríguez-Lázaro, D., Oniciuc, E.-A., García, P. G., Gallego, D., Fernández-Natal, I., Dominguez-Gil, M., Eiros-Bouza, J. M., Wagner, M., Nicolau, A. I., & Hernández, M. (2017). Detection and characterization of Staphylococcus aureus and Methicillin-resistant S. aureus in foods confiscated in EU borders. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.01344
Şanlıbaba, P. (2022). Prevalence, antibiotic resistance, and enterotoxin production of
Staphylococcus aureus isolated from retail raw beef, sheep, and lamb meat in
Turkey. International Journal of Food Microbiology, 361, Article 109461. https://doi.org/10.1016/j.ijfoodmicro.2021.109461
Sina, H., Baba-Moussa, F., Kayodé, A. P., Noumavo, P. A., Sezan, A., Hounhouigan, J. D., Kotchoni, S. O., Prévost, G., & Baba-Moussa, L. (2011). Characterization of Staphylococcus aureus isolated from street foods: Toxin profile and prevalence of antibiotic resistance. Journal of Applied Biosciences, 46, 3133-3143.
Sivakumar, M., Dubal, Z. B., Kumar, A., Bhilegaonkar, K., Vinodh Kumar, O. R., Kumar, S., Kadwalia, A., Shagufta, Bi., Grace, M. R., Ramees, T. P., & Dwivedi, A. (2019). Virulent methicillin resistant Staphylococcus aureus (MRSA) in street vended foods. Journal of Food Science and Technology, 56(3), 1116-1126. https://doi.org/10.1007/s13197-019-03572-5
Tallent, S., Hait, J., Bennett W., R., & Lancette A., G. (2020). BAM Chapter 12: Staphylococcus aureus. FDA. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-12-staphylococcus-aureus
Urban-Chmiel, R., Marek, A., Stępień-Pyśniak, D., Wieczorek, K., Dec, M., Nowaczek, A.,
& Osek, J. (2022). Antibiotic resistance in bacteria-A review. Antibiotics, 11(8), 1079. https://doi.org/10.3390/antibiotics11081079
Wang, W., Baloch, Z., Jiang, T., Zhang, C., Peng, Z., Li, F., Fanning, S., Ma, A., & Xu, J. (2017). Enterotoxigenicity and antimicrobial resistance of Staphylococcus aureus isolated from retail food in China. Frontiers in Microbiology, 8. https://doi.org/10.3389/fmicb.2017.02256
Weldeselassie, M., Gugsa, G., Kumar, A., Tsegaye, Y., Awol, N., Ahmed, M., Abebe, N., & Taddele, H. (2020). Isolation and characterization of Staphylococcus aureus from food of bovine origin in Mekelle, Tigray, Ethiopia. The Open Microbiology Journal, 14(1), Article 1. https://doi.org/10.2174/1874285802014010234
Yahya Ahmed, M., Abdalbagi Ali, H., Mohammed Taher Gorish, B., Omer Ali, S., Saif Aldein Abdalrhim, E., Hamza Mergani, M., Abass Abd Elgadir, A., Khalid Mohammed, S., Omer Ahmed, S., Alsaeid Musa, N., Saeed Ahmed, A., Mohammed Abdalla, W., Fadlallah Hamedelnil, Y., Ibrahim Hashim, A., & Altayb, H. N. (2021). Molecular detection of staphylococcal enterotoxins and mecA genes products in selected food samples collected from different areas in Khartoum state. International Journal of Microbiology, 2021,
Article e5520573. https://doi.org/10.1155/2021/5520573