HÀM LƯỢNG PHENOLIC TỔNG SỐ VÀ TIỀM NĂNG SINH HỌC CỦA CAO CHIẾT METHANOL TỪ ĐỊA Y PYRENULA SP.
Nội dung chính của bài viết
Tóm tắt
Địa y thuộc chi Pyrenula có tính đa dạng cao và phân bố rộng rãi tại Việt Nam, nhưng chúng còn nhận được ít sự quan tâm trong các nghiên cứu về hóa học và hoạt tính sinh học. Do đó, sàng lọc hóa học sơ bộ cùng các hoạt tính chống oxi hóa, kháng khuẩn và ức chế enzyme α-glucosidase của địa y Pyrenula sp. thu tại Thành phố Hồ Chí Minh đã được khảo sát. Kết quả sàng lọc hóa học đã xác nhận sự hiện diện của alkaloid, terpenoid, coumarin, quinone, tannin, phenol và flavonoid trong cao chiết. Hàm lượng phenolic tổng số (TPC) được xác định là 30,26 ± 0,51 mg GAE/g cao chiết. Về hoạt tính sinh học, cao chiết thể hiện khả năng chống oxi hóa ở mức trung bình trong thử nghiệm loại gốc tự do DPPH, với giá trị IC50 là 97,4 ± 8,5 μg/mL. Bên cạnh đó, khả năng ức chế α-glucosidase của cao chiết (IC50 = 46,5 ± 3,6 μg/mL) cho thấy tiềm năng trong việc hỗ trợ điều trị bệnh đái tháo đường. Thêm vào đó, cao chiết cho thấy hoạt tính kháng khuẩn mạnh đối với Staphylococcus aureus, được xác nhận qua đường kính kháng khuẩn đạt 20,9 ± 0,1 mm và giá trị MIC là 15,62 μg/mL. Những kết quả này cho thấy địa y Pyrenula sp. là đối tượng đáng được tiếp tục nghiên cứu về các chất chuyển hóa thứ cấp và hoạt tính sinh học.
Từ khóa
kháng khuẩn, kháng oxi hóa, ức chế α-glucosidase, Pyrenula sp., hàm lượng phenolic tổng
Chi tiết bài viết
Tài liệu tham khảo
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Bui, A. T., Vo, T. P. G., & Tran, T. M. D. (2024). Alpha-glucosidase inhibitory and antioxidant activities of ethyl acetate extract from Graphis sp. mycobiont. Ho Chi Minh City University of Education Journal of Science, 21(3), 2065–2080. https://doi.org/10.54607/hcmue.js.21.3.4063(2023)
Chan, C. W. S., & Khachigian, L. M. (2013). DNAzyme Delivery Approaches in Biological Settings. http://Www.Eurekaselect.Com, 20(28), 3448–3455. https://doi.org/10.2174/09298673113209990033
Do, T.-H., Duong, T.-H., Nguyen, H. T., Nguyen, T.-H., Sichaem, J., Nguyen, C. H., Nguyen, H.-H., & Long, N. P. (2022). Biological activities of lichen-derived monoaromatic compounds. Molecules, 27(9), Article 9. https://doi.org/10.3390/molecules27092871
Do, T. H., Duong, T. H., Vu, Y. T., Tran, H. P., Nguyen, T. T. N., Sichaem, J., Nguyen, N. H., Nguyen, H. T., & Pham, D.-D. (2024). Alpha-glucosidase inhibitory compounds from Vietnamese lichen Usnea baileyi: In vitro and in silico aspects. RSC Advances, 14(44), 32624–32636. https://doi.org/10.1039/D4RA04449E
Dou, M., Liu, S., Li, J., Aptroot, A., & Jia, Z. (2024). Three new Pyrenula species with 3-septate ascospores with red or orange oil when over-mature (Ascomycota, Pyrenulales, Pyrenulaceae) from China. MycoKeys, 102, 107–125. https://doi.org/10.3897/mycokeys.102.113619
Elshikh, M., Ahmed, S., Funston, S., Dunlop, P., McGaw, M., Marchant, R., & Banat, I. M. (2016). Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnology Letters, 38(6), 1015–1019. https://doi.org/10.1007/s10529-016-2079-2
Fernández-Moriano, C., González-Burgos, E., Divakar, P. K., Crespo, A., & Gómez-Serranillos, M. P. (2016). Evaluation of the Antioxidant Capacities and Cytotoxic Effects of Ten Parmeliaceae Lichen Species. Evidence-Based Complementary and Alternative Medicine, 2016(1), 3169751. https://doi.org/10.1155/2016/3169751
Jeyakumaran, P., Perera, K., Fernando, W., Jayasinghe, L., & Ramiah, S. (2015). α-Glucosidase and α-Amylase Inhibitory Activities of Nine Sri Lankan Antidiabetic Plants. British Journal of Pharmaceutical Research, 7, 365–374. https://doi.org/10.9734/BJPR/2015/18645
Junior, I. O., Aptroot, A., & Cáceres, M. E. da S. (2021). Lichens from Monte Pascoal, Bahia, Brazil, with some new pyrenocarpous species and a key to the Pyrenula species from Brazil. The Bryologist, 124(4), 552–568. https://doi.org/10.1639/0007-2745-124.4.552
Kuete, V. (2010). Potential of Cameroonian plants and derived products against microbial infections: A review. Planta Medica, 76(14), 1479–1491. https://doi.org/10.1055/s-0030-1250027
Le, D. H., Takenaka, Y., Hamada, N., Mizushina, Y., & Tanahashi, T. (2014). Polyketides from the cultured lichen mycobiont of a Vietnamese Pyrenula sp. Journal of Natural Products, 77(6), 1404–1412. https://doi.org/10.1021/np500143k
Lücking, R., Álvaro-Alba, W. R., Moncada, B., Marín-Canchala, N. L., Tunjano, S. S., & Cárdenas-López, D. (2023). Lichens from the Colombian Amazon: 666 Taxa Including 28 new Species and 157 New Country Records Document an Extraordinary Diversity. The Bryologist, 126(2), 242–303. https://doi.org/10.1639/0007-2745-126.2.242
Miranda-González, R., Bungartz, F., Lücking, R., Gaya, E., Mendonça, C. de O., Viñas-Portilla, C., Cáceres, M. E. da S., & Herrera-Campos, M. de los A. (2022). Phylogeny of the Pyrenula ochraceoflava group (Pyrenulaceae) reveals near-cryptic diversification and the inclusion of the Mazaediothecium album aggregate. The Bryologist, 125(4), 541–557. https://doi.org/10.1639/0007-2745-125.4.541
Nguyen, T. D., Nguyen, H. L., Pham, T. P. V., Vo, T. P. G., & Tran, T. M. D. (2024). Impact of culture media and duration on antioxidant and alpha-glucosidase inhibitory activities of methanol extract from Graphis handelii mycobiont. Ho Chi Minh City University of Education Journal of Science, 21(9), 9. https://doi.org/10.54607/hcmue.js.21.9.4199(2024)
Pant, K., Joshi, P., Upreti, D. K., Nayaka, S., & Darmwal, J. (2025). Phytochemical Investigation, Antioxidant and Antimicrobial Activities of Some Parmeliaceae Lichens from Western Himalaya. Journal of Pure and Applied Microbiology, 19(1), 263–274. https://doi.org/10.22207/JPAM.19.1.17
Phuong, N., Do, N., Tai, N., Phuong, P., & Duc, T. (2017). Khảo sát hàm lượng flavonoid, alkaloid và khả năng kháng khuẩn của cao chiết cỏ Mần Trầu (Eleusine indica) [Investigation of flavonoid and alkaloid contents and antibacterial activity of Eleusine indica extracts]. Can Tho University, Journal of Science, 53, 54. https://doi.org/10.22144/ctu.jvn.2017.157
Pradhan, S., Dash, S., Parida, S., Sahoo, B., & Rath, B. (2023). Antioxidant and antimicrobial activities and GC/MS-based phytochemical analysis of two traditional Lichen species Trypethellium virens and Phaeographis dendritica. Journal of Genetic Engineering and Biotechnology, 21(1), 41. https://doi.org/10.1186/s43141-023-00490-0
Pradhan, S., Dash, S., Sahoo, B., Parida, S., & Rath, B. (2024). Screening and characterization of bioactive compounds from two epiphytic microlichen and evaluation of their in vitro antioxidant activity. Future Journal of Pharmaceutical Sciences, 10(1), 10. https://doi.org/10.1186/s43094-024-00584-7
Proença, C., Freitas, M., Ribeiro, D., Oliveira, E. F. T., Sousa, J. L. C., Tomé, S. M., Ramos, M. J., Silva, A. M. S., Fernandes, P. A., & Fernandes, E. (2017). α-Glucosidase inhibition by flavonoids: An in vitro and in silico structure–activity relationship study. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 1216–1228. https://doi.org/10.1080/14756366.2017.1368503
Rashmi, S., & Rajkumar, H. (2014). Preliminary phytochemical screening of different solvent extracts of lichens from Kodagu district, Karnataka. Journal of Pharmacognosy and Phytochemistry. https://www.semanticscholar.org/paper/Preliminary-phytochemical-screening-of-different-of-Rashmi-Rajkumar/99d8c75616c30b141dbe8bb6441c979820a7aea2
Sargsyan, R., Gasparyan, A., Tadevosyan, G., & Panosyan, H. (2021). Antimicrobial and antioxidant potentials of non-cytotoxic extracts of corticolous lichens sampled in Armenia. AMB Express, 11(1), 110. https://doi.org/10.1186/s13568-021-01271-z
Sharma, B. P., Rani, J. J., Vimala, V., Sable, P. D., S. Kumar, S. K., Marndi, S., & Das, K. (2025). Bioactive Compounds of Medicinal Plants: Structure, Function, and Bioactivity. https://doi.org/10.5281/zenodo.17480458
Soto-Medina, E., Aptroot, A., & Lücking, R. (2023). New Species of Lichen for Colombia Tropical Dry Forest. Cryptogamie, Mycologie, 44(7), 103–107. https://doi.org/10.5252/cryptogamie-mycologie2023v44a7
Takenaka, Y., Tanahashi, T., Nagakura, N., & Hamada, N. (2000). Production of xanthones with free radical scavenging properties, emodin and sclerotiorin by the cultured lichen mycobionts of Pyrenula japonica. Zeitschrift Fur Naturforschung. C, Journal of Biosciences, 55(11–12), 910–914. https://doi.org/10.1515/znc-2000-11-1211
Torres-Benítez, A., Ortega-Valencia, J. E., Jara-Pinuer, N., Sanchez, M., Vargas-Arana, G., Gómez-Serranillos, M. P., & Simirgiotis, M. J. (2023). Antioxidant and antidiabetic activity and phytoconstituents of lichen extracts with temperate and polar distribution. Frontiers in Pharmacology, 14. https://doi.org/10.3389/fphar.2023.1251856
Vo, T. K. N., Nguyen, T. H., Tran, H. D., Nguyen, D. D., & Nguyen, T. N. M. (2017). Khảo sát hàm lượng phenolic tổng, flavonoid tổng, hoạt tính chống oxy hóa và hoạt tính kháng khuẩn của cao chiết ethanol và methanol của lá và thân rễ cây Cỏ Tranh (Imperata cylindrica) [Investigation of total phenolic and flavonoid contents, antioxidant and antibacterial activities of ethanol and methanol extracts from the leaves and rhizomes of Imperata cylindrica]. Can Tho University, Journal of Science, (52), 16–22. https://doi.org/10.22144/ctu.jvn.2017.119
White, P. A. S., Oliveira, R. C. M., Oliveira, A. P., Serafini, M. R., Araújo, A. A. S., Gelain, D. P., Moreira, J. C. F., Almeida, J. R. G. S., Quintans, J. S. S., Quintans-Junior, L. J., & Santos, M. R. V. (2014). Antioxidant Activity and Mechanisms of Action of Natural Compounds Isolated from Lichens: A Systematic Review. Molecules, 19(9), 14496–14527. https://doi.org/10.3390/molecules190914496
Zakeri, Z., Junne, S., Jäger, F., Dostert, M., Otte, V., & Neubauer, P. (2022). Lichen cell factories: Methods for the isolation of photobiont and mycobiont partners for defined pure and co-cultivation. Microbial Cell Factories, 21(1), 80. https://doi.org/10.1186/s12934-022-01804-6
Aptroot, A. (2012). A world key to the species of Anthracothecium and Pyrenula. The Lichenologist, 44(1), 5–53. https://doi.org/10.1017/S0024282911000624
Bui, A. T., Vo, T. P. G., & Tran, T. M. D. (2024). Alpha-glucosidase inhibitory and antioxidant activities of ethyl acetate extract from Graphis sp. mycobiont. Ho Chi Minh City University of Education Journal of Science, 21(3), 2065–2080. https://doi.org/10.54607/hcmue.js.21.3.4063(2023)
Chan, C. W. S., & Khachigian, L. M. (2013). DNAzyme Delivery Approaches in Biological Settings. http://Www.Eurekaselect.Com, 20(28), 3448–3455. https://doi.org/10.2174/09298673113209990033
Do, T.-H., Duong, T.-H., Nguyen, H. T., Nguyen, T.-H., Sichaem, J., Nguyen, C. H., Nguyen, H.-H., & Long, N. P. (2022). Biological activities of lichen-derived monoaromatic compounds. Molecules, 27(9), Article 9. https://doi.org/10.3390/molecules27092871
Do, T. H., Duong, T. H., Vu, Y. T., Tran, H. P., Nguyen, T. T. N., Sichaem, J., Nguyen, N. H., Nguyen, H. T., & Pham, D.-D. (2024). Alpha-glucosidase inhibitory compounds from Vietnamese lichen Usnea baileyi: In vitro and in silico aspects. RSC Advances, 14(44), 32624–32636. https://doi.org/10.1039/D4RA04449E
Dou, M., Liu, S., Li, J., Aptroot, A., & Jia, Z. (2024). Three new Pyrenula species with 3-septate ascospores with red or orange oil when over-mature (Ascomycota, Pyrenulales, Pyrenulaceae) from China. MycoKeys, 102, 107–125. https://doi.org/10.3897/mycokeys.102.113619
Elshikh, M., Ahmed, S., Funston, S., Dunlop, P., McGaw, M., Marchant, R., & Banat, I. M. (2016). Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnology Letters, 38(6), 1015–1019. https://doi.org/10.1007/s10529-016-2079-2
Fernández-Moriano, C., González-Burgos, E., Divakar, P. K., Crespo, A., & Gómez-Serranillos, M. P. (2016). Evaluation of the Antioxidant Capacities and Cytotoxic Effects of Ten Parmeliaceae Lichen Species. Evidence-Based Complementary and Alternative Medicine, 2016(1), 3169751. https://doi.org/10.1155/2016/3169751
Jeyakumaran, P., Perera, K., Fernando, W., Jayasinghe, L., & Ramiah, S. (2015). α-Glucosidase and α-Amylase Inhibitory Activities of Nine Sri Lankan Antidiabetic Plants. British Journal of Pharmaceutical Research, 7, 365–374. https://doi.org/10.9734/BJPR/2015/18645
Junior, I. O., Aptroot, A., & Cáceres, M. E. da S. (2021). Lichens from Monte Pascoal, Bahia, Brazil, with some new pyrenocarpous species and a key to the Pyrenula species from Brazil. The Bryologist, 124(4), 552–568. https://doi.org/10.1639/0007-2745-124.4.552
Kuete, V. (2010). Potential of Cameroonian plants and derived products against microbial infections: A review. Planta Medica, 76(14), 1479–1491. https://doi.org/10.1055/s-0030-1250027
Le, D. H., Takenaka, Y., Hamada, N., Mizushina, Y., & Tanahashi, T. (2014). Polyketides from the cultured lichen mycobiont of a Vietnamese Pyrenula sp. Journal of Natural Products, 77(6), 1404–1412. https://doi.org/10.1021/np500143k
Lücking, R., Álvaro-Alba, W. R., Moncada, B., Marín-Canchala, N. L., Tunjano, S. S., & Cárdenas-López, D. (2023). Lichens from the Colombian Amazon: 666 Taxa Including 28 new Species and 157 New Country Records Document an Extraordinary Diversity. The Bryologist, 126(2), 242–303. https://doi.org/10.1639/0007-2745-126.2.242
Miranda-González, R., Bungartz, F., Lücking, R., Gaya, E., Mendonça, C. de O., Viñas-Portilla, C., Cáceres, M. E. da S., & Herrera-Campos, M. de los A. (2022). Phylogeny of the Pyrenula ochraceoflava group (Pyrenulaceae) reveals near-cryptic diversification and the inclusion of the Mazaediothecium album aggregate. The Bryologist, 125(4), 541–557. https://doi.org/10.1639/0007-2745-125.4.541
Nguyen, T. D., Nguyen, H. L., Pham, T. P. V., Vo, T. P. G., & Tran, T. M. D. (2024). Impact of culture media and duration on antioxidant and alpha-glucosidase inhibitory activities of methanol extract from Graphis handelii mycobiont. Ho Chi Minh City University of Education Journal of Science, 21(9), 9. https://doi.org/10.54607/hcmue.js.21.9.4199(2024)
Pant, K., Joshi, P., Upreti, D. K., Nayaka, S., & Darmwal, J. (2025). Phytochemical Investigation, Antioxidant and Antimicrobial Activities of Some Parmeliaceae Lichens from Western Himalaya. Journal of Pure and Applied Microbiology, 19(1), 263–274. https://doi.org/10.22207/JPAM.19.1.17
Phuong, N., Do, N., Tai, N., Phuong, P., & Duc, T. (2017). Khảo sát hàm lượng flavonoid, alkaloid và khả năng kháng khuẩn của cao chiết cỏ Mần Trầu (Eleusine indica) [Investigation of flavonoid and alkaloid contents and antibacterial activity of Eleusine indica extracts]. Can Tho University, Journal of Science, 53, 54. https://doi.org/10.22144/ctu.jvn.2017.157
Pradhan, S., Dash, S., Parida, S., Sahoo, B., & Rath, B. (2023). Antioxidant and antimicrobial activities and GC/MS-based phytochemical analysis of two traditional Lichen species Trypethellium virens and Phaeographis dendritica. Journal of Genetic Engineering and Biotechnology, 21(1), 41. https://doi.org/10.1186/s43141-023-00490-0
Pradhan, S., Dash, S., Sahoo, B., Parida, S., & Rath, B. (2024). Screening and characterization of bioactive compounds from two epiphytic microlichen and evaluation of their in vitro antioxidant activity. Future Journal of Pharmaceutical Sciences, 10(1), 10. https://doi.org/10.1186/s43094-024-00584-7
Proença, C., Freitas, M., Ribeiro, D., Oliveira, E. F. T., Sousa, J. L. C., Tomé, S. M., Ramos, M. J., Silva, A. M. S., Fernandes, P. A., & Fernandes, E. (2017). α-Glucosidase inhibition by flavonoids: An in vitro and in silico structure–activity relationship study. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 1216–1228. https://doi.org/10.1080/14756366.2017.1368503
Rashmi, S., & Rajkumar, H. (2014). Preliminary phytochemical screening of different solvent extracts of lichens from Kodagu district, Karnataka. Journal of Pharmacognosy and Phytochemistry. https://www.semanticscholar.org/paper/Preliminary-phytochemical-screening-of-different-of-Rashmi-Rajkumar/99d8c75616c30b141dbe8bb6441c979820a7aea2
Sargsyan, R., Gasparyan, A., Tadevosyan, G., & Panosyan, H. (2021). Antimicrobial and antioxidant potentials of non-cytotoxic extracts of corticolous lichens sampled in Armenia. AMB Express, 11(1), 110. https://doi.org/10.1186/s13568-021-01271-z
Sharma, B. P., Rani, J. J., Vimala, V., Sable, P. D., S. Kumar, S. K., Marndi, S., & Das, K. (2025). Bioactive Compounds of Medicinal Plants: Structure, Function, and Bioactivity. https://doi.org/10.5281/zenodo.17480458
Soto-Medina, E., Aptroot, A., & Lücking, R. (2023). New Species of Lichen for Colombia Tropical Dry Forest. Cryptogamie, Mycologie, 44(7), 103–107. https://doi.org/10.5252/cryptogamie-mycologie2023v44a7
Takenaka, Y., Tanahashi, T., Nagakura, N., & Hamada, N. (2000). Production of xanthones with free radical scavenging properties, emodin and sclerotiorin by the cultured lichen mycobionts of Pyrenula japonica. Zeitschrift Fur Naturforschung. C, Journal of Biosciences, 55(11–12), 910–914. https://doi.org/10.1515/znc-2000-11-1211
Torres-Benítez, A., Ortega-Valencia, J. E., Jara-Pinuer, N., Sanchez, M., Vargas-Arana, G., Gómez-Serranillos, M. P., & Simirgiotis, M. J. (2023). Antioxidant and antidiabetic activity and phytoconstituents of lichen extracts with temperate and polar distribution. Frontiers in Pharmacology, 14. https://doi.org/10.3389/fphar.2023.1251856
Vo, T. K. N., Nguyen, T. H., Tran, H. D., Nguyen, D. D., & Nguyen, T. N. M. (2017). Khảo sát hàm lượng phenolic tổng, flavonoid tổng, hoạt tính chống oxy hóa và hoạt tính kháng khuẩn của cao chiết ethanol và methanol của lá và thân rễ cây Cỏ Tranh (Imperata cylindrica) [Investigation of total phenolic and flavonoid contents, antioxidant and antibacterial activities of ethanol and methanol extracts from the leaves and rhizomes of Imperata cylindrica]. Can Tho University, Journal of Science, (52), 16–22. https://doi.org/10.22144/ctu.jvn.2017.119
White, P. A. S., Oliveira, R. C. M., Oliveira, A. P., Serafini, M. R., Araújo, A. A. S., Gelain, D. P., Moreira, J. C. F., Almeida, J. R. G. S., Quintans, J. S. S., Quintans-Junior, L. J., & Santos, M. R. V. (2014). Antioxidant Activity and Mechanisms of Action of Natural Compounds Isolated from Lichens: A Systematic Review. Molecules, 19(9), 14496–14527. https://doi.org/10.3390/molecules190914496
Zakeri, Z., Junne, S., Jäger, F., Dostert, M., Otte, V., & Neubauer, P. (2022). Lichen cell factories: Methods for the isolation of photobiont and mycobiont partners for defined pure and co-cultivation. Microbial Cell Factories, 21(1), 80. https://doi.org/10.1186/s12934-022-01804-6