Production and characterization of sophorolipids by Candida bombicola using catfish fat

Abstract

Catfish fat is a high nutrition by-product of seafood processing industry. In this work, catfish fat has been used as a sustainable and economical raw material for sophorolipids production by Candida bombicola. Sophorolipids yield was maximum as 21.8g/L after 7 days of fermentation at 25^oC, pH 6, 180 rpm. The obtained sophorolipids was to contained the main component as lactonic sophorolipids, which has been confirmed by Thin layer chromatography (TLC). Sophorolipids also exhibited the ability to resistant Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and showed the ability of free radical scavenging the dose dependent manner with IC₅₀ was 4.45 mg/ml. These results suggested that sophorolipids could be used in health care products and cosmetic. Catfish fat could be used as the low cost hydrophobic carbon source to replace fatty acid for sophorolipids production.