EFFECTS OF UVA LIGHT EXPOSURE ON THE BODY WEIGHT, THE BLOOD CELLS AND INTERNAL ORGANS OF ALBINO MOUSE (Mus musculus var. albino)
Main Article Content
Abstract
In Vietnam, most of the studies on effects caused by UV lights have focused mainly on skin; especially, there has not published any paper on the effect of UVA lights on the body weight, the blood cells, and internal organs. This study aims to evaluate the influences of UVA light exposure on the body weight, the blood cells, and internal organs of albino mouse. Female mice (six-week-old) were shaved the hair of the dorsal skin which was then exposed to a single dose of UVA for 3, 6 and 9 hours to compare with the control mice for eight weeks. The results show that the weight body tended to decrease linearly when the exposure time is longer; the number of red blood cells increased sharply after 4 weeks and decreased sharply after 8 weeks (especially for thoses with 6 and 9 hour exposure time); the number of white blood cells increased irregularly after 4 weeks but decreased linearly after when the exposure time is longer; the number of platelets increased sharply after 4 weeks when the exposure time is longer but decreased sharply after 8 weeks when the exposure time is longer; the structures of the liver, kidney, and spleen were injured mildly for those with 3 hour exposure and badly injured for those with 6 and 9 hour exposure.
Keywords
Histopathology, Mice blood cell, Mus musculus var. albino, UVA, Ultraviolet radiation
Article Details
References
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Broucek, J., & Kovalcik, K. (1989). The effect of excessive artificial UV radiation on the measurement of blood parameters and phagocytosis in calves. DTW. Deutsche tierarztliche Wochenschrift, 96(6), 318-320.
Crosby, W. H. (1959). Normal functions of the spleen relative to red blood cells: a review. Blood, 14(4), 399-408.
Doan, S. N. (2016). Study on the structural changes of the liver, spleen white mice (swiss) under the effect of ultraviolet [Nghien cuu su thay doi cau truc cua mo, gan, lach chuot nhat trang (Swiss) duoi tac dung cua tia tu ngoai]. Journal of Science and Technology, Hue University of Sciences, 4(1), 73-82.
Ellinger, F. (1938). Acetonitril Test for Thyroid According to Reid Hunt and Irradiation With Ultraviolet Light. Radiologica, 3, 195.
Ellinger, F. (1939). The Influence of Ultra-violet Rays on the Body Weight. Radiology, 32(2),
157-160. doi: 10.1148/32.2.157
Geldenhuys, S., Hart, P. H., Endersby, R., Jacoby, P., Feelisch, M., Weller, R. B., . . . & Gorman, S. J. D. (2014). Ultraviolet radiation suppresses obesity and symptoms of metabolic syndrome independently of vitamin D in mice fed a high-fat diet. 63(11), 3759-3769.
Hamblin, M. R. (2017). Ultraviolet Irradiation of Blood: “The Cure That Time Forgot”? Ultraviolet Light in Human Health, Diseases and Environment (pp. 295-309): Springer.
James G. Fox, S. W. B., Muriel, T. Davisson, Christian, E. Newcomer, Fred, W. Quimby, & Abigail L. Smith,. (2007). The Mouse in Biomedical Research (Vol. III): Elsevier Inc.
Kabat, I. A., Sysa, J., Zakrzewska, I., & Leyko, W. (1976). Effect of UV-irradiation of shifts of energy-rich phosphate compounds: ADP, ATP and AXP in human red blood cells represented by a trigonometrical polynomial. Zentralbl Bakteriol Orig B, 162(3-4), 393-401.
Komatsu, T., Sasaki, S., Manabe, Y., Hirata, T., & Sugawara, T. (2017). Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice. PLoS ONE, 12(2), e0171178. doi: 10.1371/journal.pone.0171178
Krotz, F., Sohn, H. Y., & Pohl, U. (2004). Reactive oxygen species: players in the
platelet game. Arterioscler Thromb Vasc Biol, 24(11), 1988-1996. doi: 10.1161/01.ATV.0000145574.90840.7d
Maldague, P. (1969). Comparative study of experimentally induced cancer of the kidney in mice and rats with X-rays. Paper presented at the Proceedings of a symposium on radiation-induced cancer organized by The international atomic energy agency in collaboration with the world health organization and held in Athens, 28 april - 2 May 1969
McGarry, M. P., Protheroe, C. A., & Lee, J. J. (2010). Mouse hematology: Cold Spring Harbor Laboratory Press.
McMillan, T., Leatherman, E., Ridley, A., Shorrocks, J., Tobi, S., Whiteside, J. J. J. o. P., & Pharmacology. (2008). Cellular effects of long wavelength UV light (UVA) in mammalian cells. 60(8), 969-976.
Moysan, A., Marquis, I., Gaboriau, F., Santus, R., Dubertret, L., & Morlière, P. J. J. o. i. d. (1993). Ultraviolet A–induced lipid peroxidation and antioxidant defense systems in cultured human skin fibroblasts. 100(5), 692-698.
Mulero, M., Rodriguez-Yanes, E., Nogues, M. R., Giralt, M., Romeu, M., Gonzalez, S., & Mallol, J. (2008). Polypodium leucotomos extract inhibits glutathione oxidation and prevents Langerhans cell depletion induced by UVB/UVA radiation in a hairless rat model. Exp Dermatol, 17(8), 653-658. doi: 10.1111/j.1600-0625.2007.00684.x
Navarro, J., Obrador, E., Pellicer, J. A., Aseni, M., Vina, J., & Estrela, J. M. (1997). Blood glutathione as an index of radiation-induced oxidative stress in mice and humans. [Research Support, Non-U.S. Gov't]. Free Radic Biol Med, 22(7), 1203-1209. doi: 10.1016/s0891-5849(96)00554-0
Nguyen, T. T. H., & Vo, V. T. (2019). Laboratory practice human and animal physiology [Thuc hanh sinh li hoc nguoi va dong vat]. HCMC University of Education Publisher
Park, H. M., Shon, J. C., Lee, M. Y., Liu, K. H., Kim, J. K., Lee, S. J., & Lee, C. H. (2014). Mass spectrometry-based metabolite profiling in the mouse liver following exposure to ultraviolet B radiation. PLoS ONE, 9(10), e109479. doi: 10.1371/journal.pone.0109479
Pillai, S., Oresajo, C., & Hayward, J. (2005). Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review. Int J Cosmet Sci, 27(1), 17-34.
doi: 10.1111/j.1467-2494.2004.00241.x
Pohle, E. A., & Bunting, C. J. A. R. (1932). Studies of the Effect of Roentgen Rays on the Liver Histological Changes in the Liver of Rats Following Exposure to Single Graded Doses of Filtered Roentgen Rays. 13(2), 117-124.
Robbins, M. E., Zhao, W., Davis, C. S., Toyokuni, S., & Bonsib, S. M. J. M. (2002). Radiation-induced kidney injury: a role for chronic oxidative stress?, 33(2), 133-141.
Sanforn, H. N. (1928). Effect of Ultraviolet Light on the Blood of New-Born Infants: II. Erythrocytes and Hemoglobin. American Journal of Diseases of Children, 35(1), 9-13.
Slominski, A. T., Zmijewski, M. A., Skobowiat, C., Zbytek, B., Slominski, R. M., & Steketee, J. D. (2012). Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system. Adv Anat Embryol Cell Biol, 212, v, vii, 1-115.
Somosy, Z. (2000). Radiation response of cell organelles. Micron, 31(2), 165-181.
Svobodova, A., Walterova, D., & Vostalova, J. (2006). Ultraviolet light induced alteration to the skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 150(1), 25-38.
Svobodová, A. R., Galandáková, A., Sˇianská, J., dolezˇal, D., Ulrichová, J., & Vostálováa, J. (2011). Acute exposure to solar simulated ultraviolet radiation affects oxidative stress-related biomarkers in skin, liver and blood of hairless mice. Biol Pharm Bull, 34(4), 471-479.
doi: 10.1248/bpb.34.471
Tekin, S., Turker, H., Guven, T., & Yel, M. (2016). The effects of ultraviolet C radiation on the ultrastructure of the liver cells of mole rats. Ultrastruct Pathol, 40(1), 51-56.
doi: 10.3109/01913123.2015.1088909
Turker, H. (2014). Haematological effects of ultraviolet-c radiation on swiss albino mice. International Journal of Toxicology and Applied Pharmacology, 4(1), 17-22.
Türker, H., & Yel, M. (2014). Effects of ultraviolet radiation on mole rats kidney: A histopathologic and ultrastructural study. Journal of Radiation Research and Applied Sciences, 7(2), 182-187. doi: 10.1016/j.jrras.2014.02.004
Verschooten, L., Claerhout, S., Van Laethem, A., Agostinis, P., & Garmyn, M. (2006). New strategies of photoprotection. Photochem Photobiol, 82(4), 1016-1023. doi: 10.1562/2006-04-27-ir-884.1
Wang, L., Zhang, X., Li, Y. X., Xu, L. Q., Li, C. L., Zhang, Z. B., . . . & Li, Y. C. (2016). Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin. Evid Based Complement Alternat Med, 2016, 9623957. doi: 10.1155/2016/9623957
Wondrak, G. T., Jacobson, M. K., & Jacobson, E. L. (2006). Endogenous UVA-photosensitizers: mediators of skin photodamage and novel targets for skin photoprotection. Photochem Photobiol Sci, 5(2), 215-237. doi: 10.1039/b504573h
Yazdi, G. T., Jaafar, M. S., & Abdullah, W. Z. (2016). The Effects of Ultraviolet Light Irradiation on Hematological and Morphological Characteristics and Potassium Level of Human Blood for Transfusion Associated Graft Versus Host Disease Prevention. Biomedical Technologies, 3(3-4), 80-85.
Broucek, J., & Kovalcik, K. (1989). The effect of excessive artificial UV radiation on the measurement of blood parameters and phagocytosis in calves. DTW. Deutsche tierarztliche Wochenschrift, 96(6), 318-320.
Crosby, W. H. (1959). Normal functions of the spleen relative to red blood cells: a review. Blood, 14(4), 399-408.
Doan, S. N. (2016). Study on the structural changes of the liver, spleen white mice (swiss) under the effect of ultraviolet [Nghien cuu su thay doi cau truc cua mo, gan, lach chuot nhat trang (Swiss) duoi tac dung cua tia tu ngoai]. Journal of Science and Technology, Hue University of Sciences, 4(1), 73-82.
Ellinger, F. (1938). Acetonitril Test for Thyroid According to Reid Hunt and Irradiation With Ultraviolet Light. Radiologica, 3, 195.
Ellinger, F. (1939). The Influence of Ultra-violet Rays on the Body Weight. Radiology, 32(2),
157-160. doi: 10.1148/32.2.157
Geldenhuys, S., Hart, P. H., Endersby, R., Jacoby, P., Feelisch, M., Weller, R. B., . . . & Gorman, S. J. D. (2014). Ultraviolet radiation suppresses obesity and symptoms of metabolic syndrome independently of vitamin D in mice fed a high-fat diet. 63(11), 3759-3769.
Hamblin, M. R. (2017). Ultraviolet Irradiation of Blood: “The Cure That Time Forgot”? Ultraviolet Light in Human Health, Diseases and Environment (pp. 295-309): Springer.
James G. Fox, S. W. B., Muriel, T. Davisson, Christian, E. Newcomer, Fred, W. Quimby, & Abigail L. Smith,. (2007). The Mouse in Biomedical Research (Vol. III): Elsevier Inc.
Kabat, I. A., Sysa, J., Zakrzewska, I., & Leyko, W. (1976). Effect of UV-irradiation of shifts of energy-rich phosphate compounds: ADP, ATP and AXP in human red blood cells represented by a trigonometrical polynomial. Zentralbl Bakteriol Orig B, 162(3-4), 393-401.
Komatsu, T., Sasaki, S., Manabe, Y., Hirata, T., & Sugawara, T. (2017). Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice. PLoS ONE, 12(2), e0171178. doi: 10.1371/journal.pone.0171178
Krotz, F., Sohn, H. Y., & Pohl, U. (2004). Reactive oxygen species: players in the
platelet game. Arterioscler Thromb Vasc Biol, 24(11), 1988-1996. doi: 10.1161/01.ATV.0000145574.90840.7d
Maldague, P. (1969). Comparative study of experimentally induced cancer of the kidney in mice and rats with X-rays. Paper presented at the Proceedings of a symposium on radiation-induced cancer organized by The international atomic energy agency in collaboration with the world health organization and held in Athens, 28 april - 2 May 1969
McGarry, M. P., Protheroe, C. A., & Lee, J. J. (2010). Mouse hematology: Cold Spring Harbor Laboratory Press.
McMillan, T., Leatherman, E., Ridley, A., Shorrocks, J., Tobi, S., Whiteside, J. J. J. o. P., & Pharmacology. (2008). Cellular effects of long wavelength UV light (UVA) in mammalian cells. 60(8), 969-976.
Moysan, A., Marquis, I., Gaboriau, F., Santus, R., Dubertret, L., & Morlière, P. J. J. o. i. d. (1993). Ultraviolet A–induced lipid peroxidation and antioxidant defense systems in cultured human skin fibroblasts. 100(5), 692-698.
Mulero, M., Rodriguez-Yanes, E., Nogues, M. R., Giralt, M., Romeu, M., Gonzalez, S., & Mallol, J. (2008). Polypodium leucotomos extract inhibits glutathione oxidation and prevents Langerhans cell depletion induced by UVB/UVA radiation in a hairless rat model. Exp Dermatol, 17(8), 653-658. doi: 10.1111/j.1600-0625.2007.00684.x
Navarro, J., Obrador, E., Pellicer, J. A., Aseni, M., Vina, J., & Estrela, J. M. (1997). Blood glutathione as an index of radiation-induced oxidative stress in mice and humans. [Research Support, Non-U.S. Gov't]. Free Radic Biol Med, 22(7), 1203-1209. doi: 10.1016/s0891-5849(96)00554-0
Nguyen, T. T. H., & Vo, V. T. (2019). Laboratory practice human and animal physiology [Thuc hanh sinh li hoc nguoi va dong vat]. HCMC University of Education Publisher
Park, H. M., Shon, J. C., Lee, M. Y., Liu, K. H., Kim, J. K., Lee, S. J., & Lee, C. H. (2014). Mass spectrometry-based metabolite profiling in the mouse liver following exposure to ultraviolet B radiation. PLoS ONE, 9(10), e109479. doi: 10.1371/journal.pone.0109479
Pillai, S., Oresajo, C., & Hayward, J. (2005). Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review. Int J Cosmet Sci, 27(1), 17-34.
doi: 10.1111/j.1467-2494.2004.00241.x
Pohle, E. A., & Bunting, C. J. A. R. (1932). Studies of the Effect of Roentgen Rays on the Liver Histological Changes in the Liver of Rats Following Exposure to Single Graded Doses of Filtered Roentgen Rays. 13(2), 117-124.
Robbins, M. E., Zhao, W., Davis, C. S., Toyokuni, S., & Bonsib, S. M. J. M. (2002). Radiation-induced kidney injury: a role for chronic oxidative stress?, 33(2), 133-141.
Sanforn, H. N. (1928). Effect of Ultraviolet Light on the Blood of New-Born Infants: II. Erythrocytes and Hemoglobin. American Journal of Diseases of Children, 35(1), 9-13.
Slominski, A. T., Zmijewski, M. A., Skobowiat, C., Zbytek, B., Slominski, R. M., & Steketee, J. D. (2012). Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system. Adv Anat Embryol Cell Biol, 212, v, vii, 1-115.
Somosy, Z. (2000). Radiation response of cell organelles. Micron, 31(2), 165-181.
Svobodova, A., Walterova, D., & Vostalova, J. (2006). Ultraviolet light induced alteration to the skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 150(1), 25-38.
Svobodová, A. R., Galandáková, A., Sˇianská, J., dolezˇal, D., Ulrichová, J., & Vostálováa, J. (2011). Acute exposure to solar simulated ultraviolet radiation affects oxidative stress-related biomarkers in skin, liver and blood of hairless mice. Biol Pharm Bull, 34(4), 471-479.
doi: 10.1248/bpb.34.471
Tekin, S., Turker, H., Guven, T., & Yel, M. (2016). The effects of ultraviolet C radiation on the ultrastructure of the liver cells of mole rats. Ultrastruct Pathol, 40(1), 51-56.
doi: 10.3109/01913123.2015.1088909
Turker, H. (2014). Haematological effects of ultraviolet-c radiation on swiss albino mice. International Journal of Toxicology and Applied Pharmacology, 4(1), 17-22.
Türker, H., & Yel, M. (2014). Effects of ultraviolet radiation on mole rats kidney: A histopathologic and ultrastructural study. Journal of Radiation Research and Applied Sciences, 7(2), 182-187. doi: 10.1016/j.jrras.2014.02.004
Verschooten, L., Claerhout, S., Van Laethem, A., Agostinis, P., & Garmyn, M. (2006). New strategies of photoprotection. Photochem Photobiol, 82(4), 1016-1023. doi: 10.1562/2006-04-27-ir-884.1
Wang, L., Zhang, X., Li, Y. X., Xu, L. Q., Li, C. L., Zhang, Z. B., . . . & Li, Y. C. (2016). Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin. Evid Based Complement Alternat Med, 2016, 9623957. doi: 10.1155/2016/9623957
Wondrak, G. T., Jacobson, M. K., & Jacobson, E. L. (2006). Endogenous UVA-photosensitizers: mediators of skin photodamage and novel targets for skin photoprotection. Photochem Photobiol Sci, 5(2), 215-237. doi: 10.1039/b504573h
Yazdi, G. T., Jaafar, M. S., & Abdullah, W. Z. (2016). The Effects of Ultraviolet Light Irradiation on Hematological and Morphological Characteristics and Potassium Level of Human Blood for Transfusion Associated Graft Versus Host Disease Prevention. Biomedical Technologies, 3(3-4), 80-85.