THE BIOCONTROL OF Corynespora cassiicola CAUSING THE CORYNESPORA LEAF FALL (CLF) DISEASE ON RUBBER TREE BY Bacillus sp. S29 FROM IN VITRO, EX-VIVO TO NURSERY SCALE
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Abstract
This study aims at evaluating the biological controlling of Corynespora leaf fall disease in rubber trees caused by Corynespora cassiicola by Bacillus sp. S29 bacteria from in-vitro to scale greenhouse. The fermented Bacillus sp. S29 was able to control C. cassiicola biologically at the in vitro and on rubber leaves at the ex-vivo level. The Bacillus sp. S29 was identified by the combination of molecular biology and several analyses of biochemical methods. The result was that the Bacillus sp. S29 was similar with B. subtilis. At the scale greenhouse, B. subtilis S29 was also able to control C. cassiicola biologically. It is concluded that the B. subtilis S29 can potentially help to prevent Corynespora leaf fall disease in rubbers.
Keywords
Bacillus subtilis S29, Corynespora cassiicola, ex-vivo, nursery scale
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References
Nguyen, V. M., Mai, H. P., Vo, N. Y. N., Duong, N. L., & Nguyen, A. N. (2014). Sang loc vi sinh vat noi sinh cay cao su co kha nang kiem soat sinh hoc vi nam Corynespora cassicola [Screening of endophytes from rubber trees (Hevea brasiliensis) for biological control of Corynespora cassiicola]. Journal of Biology, 36(1se), 173-179. doi: 10.15625/0866-7160/v36n1se.4390
Nguyen, A. N., Jugah, K., Sunderasan, E., Mohd, P. A., Adam, M., & Suhaimi, N. (2008). Morphological and inter simple sequence repeat (ISSR) markers analyses of Corynespora cassiicola isolates from rubber plantations in Malaysia. Mycopathologia, 166(4), 189-201. doi: 10.1007/s11046-008-9138-8
Purwantara, A. A (1987). Histological study of Hevea leaves infected by Corynespora cassiicola (Berk. & Curt.) Wei. Menara Perkebunan, 55, 47-4900.
To, H. S., Nguyen, T. D. T., Huynh, T. T. N., Vo, N. Y. N., Duong, N. L., & Nguyen, V. M. (2013). Sang loc Bacillus co kha nang kiem soat sinh hoc nam Corynespora cassiicola [Screening of Bacillus spp for biocontrol Corynespora cassiicola and stimulate plant growth]. National Biotechnology Conference 2013, 2, 513-517.
Ruiz, -G. C., Bejar, V., Martinez-Checa, F., Llamas, I., & Quesada, E. (2005). Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Velez in Malaga, southern Spain. International Journal of Systematic and Evolutionary Microbiology, 55(1), 191-195. doi:10.1099/ijs.0.63310-0
Vietnam Rubber Group (2012), Quy trinh ki thuat cay cao su [Rubber tree technical process]. Vietnam Rubber Group, 92-93.
Zhao, Z. Z., Wang, Q., Wang, K. B. K., Liu, C. H., & Gu, Y. (2010). Study of the antifungal activity of Bacillus vallismortis ZZ185 in vitro and identification of its antifungal components. Bioresource Technolog, 101, 292-297. doi: 10.1016/j.biortech.2009.07.071
Zhao, X., Han Y., Tan, X., Wang, J., & Zhou, Z. (2014). Optimization of Antifungal Lipopeptide Production from Bacillus sp. BH072 by Response Surface Methodology. Journal of Microbiology, 1-9. doi:10.1007/s12275-014-3354-3