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Date Published: 30/12/2020
Online Published: 31/12/2020
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DOI: https://doi.org/10.54607/hcmue.js.17.12.2845(2020)
Issue
Vol. 17 No. 12 (2020)
Section
Articles
How to Cite
Cao , T. N., Phạm , T. M. H., & Nguyễn , T. H. (2020). CREATING A COLLECTION OF FRUCTOPHILIC LACTIC ACID BACTERIA (FLAB) FROM THE GASTROINTESTINAL TRACTS OF HONEYBEES. HCMUE Journal of Science, 17(12), 2293. https://doi.org/10.54607/hcmue.js.17.12.2845(2020)

CREATING A COLLECTION OF FRUCTOPHILIC LACTIC ACID BACTERIA (FLAB) FROM THE GASTROINTESTINAL TRACTS OF HONEYBEES

Thanh Nhẹ Cao , Thị Mai Hương Phạm , Thúy Hương Nguyễn

Main Article Content

Abstract

 

 

This study aims to isolate, name, and choose Fructophilic lactic acid bacteria (FLAB) from the gastrointestinal tracts of honeybees and also to determine their antibacterial activity against foulbrood disease bacteria in honey bees. The FLABs are oriented to create probiotic products that are alternated or coordinated with the current antibiotic treatment in bees. Four strains were selected with high antibacterial activity against Melissococcus pluton, Paenibacillus larvae, and Paenibacillus alvei with inhibitory zones of 8,67±0,58 mm to 15,33±0,58 mm.  All the four strains had potential probiotic properties as auto-aggregation and acid intolerance. Besides, all strains showed resistance to streptomycin and kanamycin in the treatment of bee diseases so that they can be alternated or coordinated with antibiotic within the permitted limits, without affecting the beneficial microbiota. The bacteria were able to survive at 60oC involving several stresses of spray-drying conditions. All strains were identified as Lactobacillus kunkeei by sequencing of the 16S rRNA gene with from 99.24% to 99.44% identity score. The L. kunkeei M8 strain possesses the best probiotic potential amongst the four strains. Therefore, it may be used as a candidate in the applications of probiotic additives for honey bees.

 

 

Keywords

Foulbrood disease, Fructophilic lactic acid bacteria, Lactic acid bacteria, Lactobacillus kunkee

Article Details

References

Al-Ghamdi, A. (2018). Effect of gut bacterial isolates from Apis mellifera jemenitica on Paenibacillus larvae infected bee larvae. Saudi Journal of Biological Sciences, 25(2),
383-387.
Arrendondo, D. (2018). Lactobacillus kunkeei strains decreased the infection by honey bee pathogens Paenibacillus larvae and Nosema ceranae. Beneficial Microbes, 9(2), 279-290 .
Daisley, A. B. (2020). Novel probiotic approach to counter Paenibacillus larvae infection in honey. The ISME Journal, 14, 476-491.
Endo, A. (2018). Fructophilic Lactic Acid Bacteria, a Unique Group of Fructose-Fermenting Microbes . Appl Environ Microbiol, 84(19), e01290-18.
Endo, A., & Dicks, L. M. (2014). The genus Fructobacillus. In W. H. Holzapfel, & B. J. Wood (Eds.), Lactic Acid Bacteria: Biodiversity and Taxonomy (pp. 381-398). Turku, Finland: John Wiley & Sons.
Forsgren, E. (2010). European foulbrood in honey bees. Journal of Invertebrate Pathology, 103, S5-S9.
Hoben, H. J. (1982). Comparison of the pour, spread and drop plate methods for enumeration of Rhizobium spp. in inculants made from presterilized peat. Applied and Enviromental Microbiology, 44(5), 246-1247.
Huang (2017). Spray drying of probiotics and other food-grade bacteria: A review. Trends in Food Science & Technology.
Iorizzo, M., Lombardi, S. J., Ganassi, S., Testa, B., Ianiro, M., Letizia, F., ... & Sorrentino, E. (2020). Antagonistic Activity against Ascosphaera apis and Functional Properties of Lactobacillus kunkeei Strains. Antibiotics, 9(262).
Jaimee, G. (2015). Emerging resistance to aminoglycosides in lactic acid bacteria of food origin—an impending menace. Appl Microbiol Biotechnol, 100(3), 1137-1151.
Kandler, O. (1986). Genus Lactobacillus Beijerinck 1901, 212AL. Bergeys Manual of system Bacteriology, 2(1), 1209-1234.
Kos (2003). Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. Journal of Applied Microbiology, 94, 981-987.
Moore, T., Globa, L., Barbaree, J., Vodyanoy, V., & Sorokulova, I. (2013). Antagonistic activity of Bacillus bacteria against food-borne pathogens. Probiotics and healthy, 1(3).
Olofsson, A. V. (2015). The lactic acid bacteria involved in the production of bee pollen and bee bread. Journal of Apicultural Research, 48(3).
Pătruică, S. D. (2012). The Effect of Prebiotic and Probiotic Feed Supplementation on the Wax Glands of Worker Bees (Apis Mellifera). Scientific Papers: Animal Sciences and Biotechnologies, 45(2), 268-271.
Salman, S. M. (2018). Fructophilic lactic acid bacteria symbionts in honeybees - a key role to antimicrobial activities. IOSR Journal of Pharmacy and Biological Sciences, 13(1), 58-62.
Sharma (2015). Antibiotic Resistance of Lactobacillus ssp. Isolated from Commercial Probiotic Preparations . Journal of Food Safety, 36(1), 38-51.
Tou, Y. (2013). Aggregation and adhesion properties of 22 Lactobacillus strains. Journal of Dairy Science, 96(7), 4252-4257.
Ugras, S. (2017). Isolation, identification and characterization of probiotic properties of bacterium from the honey stomachs of Yigilca honeybees in Turkey. Turkish journal of entomology, 41, 253-261.
Vásquez, A., & Olofsson, T. (2009). The lactic acid bacteria involved in the production of bee pollen and bee bread. Journal of Apicultural Research, 48(3).