SAVING MEDIA FOR PILOT – SCALE CULTIVATION OF DUNALIELLA SALINA MICROALGA IN VIET NAM
Main Article Content
Abstract
Dunaliella salina is a unicellular green microalgae capable of accumulating large carotenoid contents, in particular β-carotene under adverse culturing conditions. Four strains of D. salina N, O, J and CCAP 19/18 were used to investigate MD4, RM1, and RM2 media based on the cell density, growth rate, photosynthesis pigments chlorophyll, and carotenoid. The results showed that the cell density and growth rate of D. salina strains were high when cultured in RM1 and RM2 media using salt field water. The chlorophyll and carotenoid of D. salina strains were synthesized with the highest contents when cultured in an RM2 medium. Thus, the RM2 medium combining with salt field water and seawater stimulated D. salina cells to grow rapidly, stably, and economically, suitable for D. salina pilot-scale culture in Viet Nam.
Keywords
carotenoid, chlorophyll, Dunaliella salina, pilot-scale
Article Details
References
Andersen, R. A. (2005). Algal Culturing Techniques: Academic Press.
Atkinson, M. J., and C. Bingman. (1997). Elemental composition of commercial seasalts. Journal of Aquariculture and Aquatic Sciences, 8(2), 39-43.
Ben-Amotz, A. (1987). Effect of irradiance and nutrient deficiency on the chemical composition of Dunaliella bardawil Ben-Amotz and Avron (Volvocales, Chlorophyta). Journal of plant physiology, 131(5), 479-487.
Borowitzka, L., Moulton, T., & Borowitzka, M. (1984). The mass culture of Dunaliella salina for fine chemicals: from laboratory to pilot plant. Paper presented at the Eleventh International Seaweed Symposium.
Colusse, G. A., Mendes, C. R. B., Duarte, M. E. R., de Carvalho, J. C., & Noseda, M. D. (2020). Effects of different culture media on physiological features and laboratory scale production cost of Dunaliella salina. Biotechnology Reports, 27, e00508.
Levasseur, M., Peter A. Thompson, and Paul J. Harrison. (1993). Physiological acclimation of marine phytoplankton to different nitrogen sources 1. Journal of Phycology, 29(5), 587-595.
Lichtenthaler, H. K., & Wellburn, A. R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. In: Portland Press Ltd.
Murthy, K. C., Vanitha, A., Rajesha, J., Swamy, M. M., Sowmya, P., & Ravishankar, G. A. (2005). In vivo antioxidant activity of carotenoids from Dunaliella salina—a green microalga. Life sciences, 76(12), 1381-1390.
Prieto, A., Canavate, J. P., & García-González, M. (2011). Assessment of carotenoid production by Dunaliella salina in different culture systems and operation regimes. Journal of biotechnology, 151(2), 180-185.
Robert, A. a. (2005). Algal culturing techniques: Elsevier.
Sathasivam, R., & Juntawong, N. (2013). Modified medium for enhanced growth of Dunaliella strains. Int J Curr Sci, 5, 67-73.
Tran, D. N., Doan, N. N. T., Ho, K. Q. M., Nguyen, T. M. L., Sixto, P., Hoang, T., & Duong, D. T. (2013). A potential low cost medium for cultivation of Dunaliella salina DCCBC15 in Vietnam. Journal of Biology, 35(3), 328-332.