KHẢO SÁT PHÁT HIỆN ESCHERICHIA COLI SINH NEW DELHI METALLO-β-LACTAMASE (BlaNDM-1) TRÊN CHÓ KHỎE MẠNH Ở CÁC TRẠI THÚ CƯNG ĐẮK LẮK

Abstract

Carbapenem antibiotic, which was presented during 1980s, have acted as the last line of defense against multidrug-resistant Gram-negative organisms. The occurence of E. coli carrying the new carbapenemase: New Delhi metallo-β-lactamase (bla_NDM-1) in human has been known for many years. However, limited studies have been conducted on animal in Viet Nam. This study aimed to investigate the antimicrobial susceptibility testing of E. Coli and evaluate the phenotype and genotype of carbapenem-resistance E. coli. About 269 E. coli isolates were isolated from sixty-four rectal swabs in dogs to check the antimicrobial susceptibility testing (AST) for different 11 types of antimicrobials. In this study, we combined modified carbapenem inactivation method (mCIM) and EDTA-modified carbapenem inactivation method EDTA-CIM (eCIM) for phenotypic detection of carbapenemase producing E. coli and Polymerase Chain reaction method (PCR) for detecting and distinguishing three main types of the metallo-β-lactamases genes (NDM, VIM, IMP). The result of antimcrobial susceptibility testing by Kirby-Bauer method showed that 4/269 isolates had resistance to Carbapenem, 1/4 isolate was detected with metallo-β-lactamase by mCIM and eCIM and confirmed with NDM-1 gene by PCR. These findings provide up-to-date information on the first detection of E. coli carrying resistance genes (bla_NDM-1) in healthy dogs in Vietnam.