INVESTIGATION OF THE IRIDOID COMPOSITION OF EXTRACTS FROM DI HUANG (Rehmannia glutinosa Libosch.) ROOT USING UHPLC AND LC-MSD-Trap-SL
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Abstract
This research compares the difference of iridoid composition between the extracts collected from Di huang root (Rehmannia glutinosa Libosch.) using UHPLC and LC-MSD-Trap-SL. According to the data obtained from LC-MSD-Trap-SL, Jioglutoside B, Leonuride, and Jioglutin E were the iridoids to distinguish the variation of two extracts. Meanwhile, UHPLC analysis also demonstrated that the content of catalpol, 8-epologanic acid, and glutinoside of aqueous extract was much higher than that of ethanol extract (126; 4.46 and 11.14 times higher, respectively). Therefore, it is suggested to use water for extraction of the iridoid active compounds with high biological activity from Di huang root which contained catalpol, an iridoic glycoside dominating in Di huang root.
Keywords
catalpol, iridoid, LC-MSD-Trap-SL, Rehmannia glutinos Libosch, UHPLC
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References
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