NGHIÊN CỨU CHIẾT XUẤT, PHÂN LẬP AXIT URSOLIC TỪ LÁ CÂY ARALIA HIEPIANA VÀ KHẢO SÁT ĐIỀU KIỆN ĐÓNG GÓI VÀO HỆ TIỂU PHÂN NANO LIPID

Abstract

Ursolic acid (UA) is bioactivity that is a predominant component in  Aralia hiepiana J.Wen & Lowry, which was distributed widely in the western highlands of Vietnam. However, the application of UA faces many difficulties because of its poor solubility in water. So nanostructured lipid carrier (NLC) is an approach to increasing the water solubility and bioavailability of UA. Ursolic acid-encapsulated nanostructured lipid carrier (NLC-UA) has been prepared from inexpensive materials such as soy lecithin, Tween-80, beeswax (solid lipid phase), or soybean oil (liquid lipid phase). The results of this physical-chemical analysis (FTIR, ¹H-NMR, ¹³C-NMR DEPT, HMBC) of the isolated compound indicated that UA was successfully isolated with high purity. In addition, NLC-UA formulation using 50% soybean oil (liquid lipid phase) was the most optimal with an average particle size of 137.50 ± 0.49 nm, dispersion index (PDI) of 0.153 ± 0.03, encapsulation efficiency of 89.13± 0.33%, and payload of 15,04 ± 0,31%. The stability study also shows that the nanosystem was stable for a long time under storage conditions. The findings of this study also imply that by being encapsulated into NLC, the potential to be widely applied in the pharmaceutical and biomedical fields of UA could be improved.