ESTABLISHMENT OF A HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR QUANTIFICATION OF ISORHAMNETIN IN EXTRACT OF ANOECTOCHILUS ROXBURGHII
Main Article Content
Abstract
Anoectochilus roxburghii is a precious medicinal plant in Vietnam thanks to its pharmacological effects such as antioxidant, anti-inflammatory, hepatoprotective effects, antidiabetic, and especially anti-cancer activity. A process for quantifying isorhamnetin, an important active component, was established by high-performance liquid chromatography. The analysis was performed using a C18 column on the HPLC-DAD system with an isocratic mobile phase of acetonitrile and phosphoric acid 0,2%. The column temperature was 30ºC, injection volume - 10 µl, and detector DAD - 370 nm. The method was validated in terms of specificity, system suitability, precision, accuracy, and linear range
Keywords
Anoectochilus roxburghii, HPLC, isorhamnetin
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References
European Medicines Agency. (2005). ICH Topic Q2 (R1) Validation of Analytical Procedures. Text and Methodology, International Conference on Harmonization.
Guo, Y., Ye, Q., Yang, S., Wu, J., Ye, B., Wu, Y., . . . Zheng, C. (2019). Therapeutic effects of polysaccharides from Anoectochilus roxburghii on type II collagen-induced arthritis in rats. International Journal of Biological Macromolecules, 122, 882-892. doi: 10.1016/j.ijbiomac.2018.11.015
He, C. N., Wang, C. L., Guo, S. X., Yang, J. S., & Xiao, P. G. (2005). [Study on chemical constituents in herbs of Anoectochilus roxburghii II]. Zhongguo Zhong Yao Za Zhi, 30(10), 761-763.
Kim, J. E., Lee, D. E., Lee, K. W., Son, J. E., Seo, S. K., Li, J., . . . Lee, H. J. (2011). Isorhamnetin suppresses skin cancer through direct inhibition of MEK1 and PI3-K. Cancer Prevention Research, 4(4), 582-591. doi: 10.1158/1940-6207.CAPR-11-0032
Le, D.C., Bui, B.T., Ha, T.T.H., Ngo, T.K., & Le, Q.H. (2019). Quantification of quercetin, isorhamnetin and ferulic acid in dry extract of Anoectochilus setaceus Blume from Vietnam. International Journal of Botany Studies, 4(5), 14-18.
Li, C., Yang, X., Chen, C., Cai, S., & Hu, J. (2014). Isorhamnetin suppresses colon cancer cell growth through the PI3KAktmTOR pathway. Molecular Medicine Reports, 9(3), 935-940. doi: 10.3892/mmr.2014.1886
Li, Q., Ren, F. Q., Yang, C. L., Zhou, L. M., Liu, Y. Y., Xiao, J., . . . Wang, Z. G. (2015). Anti-proliferation effects of isorhamnetin on lung cancer cells in vitro and in vivo. Asian Pacific Journal of Cancer Prevention, 16(7), 3035-3042. doi: 10.7314/apjcp.2015.16.7.3035
Lin, C. C., Huang, P. C., & Lin, J. M. (2000). Antioxidant and hepatoprotective effects of Anoectochilus formosanus and Gynostemma pentaphyllum. The American Journal of Chinese Medicine, 28(1), 87-96. doi: 10.1142/S0192415X00000118
Shyur, L. F., Chen, C. H., Lo, C. P., Wang, S. Y., Kang, P. L., Sun, S. J., . . . Yang, N. S. (2004). Induction of apoptosis in MCF-7 human breast cancer cells by phytochemicals from Anoectochilus formosanus. Journal of Biomedical Science, 11(6), 928-939. doi: 10.1159/000081840
Wang, S. Y., Kuo, Y. H., Chang, H. N., Kang, P. L., Tsay, H. S., Lin, K. F., . . . Shyur, L. F. (2002). Profiling and characterization antioxidant activities in Anoectochilus formosanus hayata. Journal of Agricultural and Food Chemistry, 50(7), 1859-1865. doi: 10.1021/jf0113575
Wu, Y. B., Peng, M. C., Zhang, C., Wu, J. G., Ye, B. Z., Yi, J., . . . Zheng, C. J. (2020). Quantitative determination of multi-class bioactive constituents for quality assessment of ten Anoectochilus, four Goodyera and one Ludisia species in China. Chinese Herbal Medicines, 12(4), 430-439.
Yang, L. C., Hsieh, C. C., Lu, T. J., & Lin, W. C. (2014). Structurally characterized arabinogalactan from Anoectochilus formosanus as an immuno-modulator against CT26 colon cancer in BALB/c mice. Phytomedicine, 21(5), 647-655. doi: 10.1016/j.phymed.2013.10.032