EFFECTS OF GA3 ON THE GROWTH OF VD20 RICE CULTIVAR CULTURED IN VITRO ON SALT CONDITION
Main Article Content
Abstract
VD20 (Oryza sativa L.) is a special rice variety with high economic value because of its delicious, supple, and fragrant quality. However, saline intrusion due to the impact of climate change has affected the growth and yield of rice plants. In this study, we investigated the effect of salinity and GA3 at different concentrations on germination, growth, and tolerance of rice variety VD20 cultured in vitro through monitoring of growth and physiological parameters of plants after three weeks. The results showed that the higher the salt concentration, the lower the growth of rice, especially at the NaCl concentration of 10g/L. The addition of 0.5mg/L GA3 to the saline 10g/L medium helped the plants to optimally improve the growth and physiological parameters.
Keywords
GA3, in vitro, saline, growth, VD20
Article Details
References
Basalah, M. O. & Mohammad, S. (1999). Effect of salinity and plant growth regulators on seed germination of Medicago sativa L. Pakistan J. Biol. Sci., 2, 651-653.
Boucau, J., & Ungar, I. A. (1976), Hormonal control of germination under saline conditions of three halophyte taxa in genus Suaeda. Physiol Plant 36: 197–200.
Bui, T. V. (2000). Sinh li thuc vat dai cuong, phan I: dinh duong [Plant physiology, Part I: Nutrition], Ho Chi Minh City National University Puplishing House, 349 pages.
Deinlein, U., Stephan, A. B., Horie, T., Luo, W., Xu, G., & Schroeder, J. I. (2014). Plant salt-tolerance mechanisms. Trends in plant science, 19(6), 371-379.
Hoang, M. T., Nguyen, Q. T. & Vu, Q. S. (2006), Giao trinh Sinh li thuc vat [Plant physiology].
Ha Noi Agricultural Publisher.
Justin, S. H. F. W., & Armstrong, W. (1987). The anatomical characteristics of roots and plant response to soil flooding. New Phytologist, 465-495.
Le, Q. D., Nguyen, T. T., Nguyen, V. N., & Le, K. H. (2011). Tang cuong nang luc va cai bien hat giong va san xuat lua de dam bao an toan luong thuc o vung doi nui cao Viet Nam [Strengthening capacity to improve seed resources and rice production to ensure food security in the high mountains of Vietnam]. Agricultural and Rural Development Publishing House.
Mahajan, S., & Tuteja N., Cold (2005). Salinity and drought stresses: An overview”. Arch, Biochem, Biophys, 444,139-158.
Ministry of Agriculture and Rural Development (2006). Giong va Thoi vu san xuat lua o Dong bang song Cuu Long [Rice varieties and season of rice production in the Mekong Delta] Agriculture Publishing House, 99 pages.
Mizrahi, Y., Blumonfeld, A., Bittner, S., & Richmond, A. E.(1971). Abscisic acid and cytokinin content of leaves in relation to salinity and relative humidity. Plant Physiol, 48, 752-755.
Moore, C. A., Bowen, H. C., Scrase-Field, S., Knight, M. R., & White, P. J. (2002). The deposition of suberin lamellae determines the magnitude of cytosolic Ca2+ elevations in root endodermal cells subjected to cooling. Plant Journal, 30, 457-465.
Mondal, S., Singh, R. P., & Bose, B. (2013). Standardization of Culture Medium for Somatic Embryogenesis of Rice Var. MTU 7029. International journal of Bio-resource and Stress Management, 4(4), 500-505.
Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annu, Rev, Plant Biol., 59, 651-681.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culturé. Physiol Plant, 15, 473-497
National Steering Committee for Natural Disaster Prevention and Control (2020). Retrieved from http://phongchongthientai.mard.gov.vn/Pages/tom-tat-thien-tai-viet-nam-tu-dau-nam-2020-tinh-den-ngay-23-7-2020-.aspx
Negrao, S., Courtois, B., Ahmadi, N., Abreu, I., Saibo, N., & Oliveira, M. M. (2013). Recent updates on salinity stress in rice: From physiological to moleculer responses. Plant Science, 30(4), 329-377.
Nguyen, N. D. (2009). Giao trinh cay lua [Rice cirricular]. Ho Chi Minh City National University Puplishing House.
Ngo, D. T. (2006), Nghien cuu phat trien giong lua chong chiu man cho vung Dong bang song Cuu Long [Research and development of salt-tolerant rice varieties for the Mekong Delta].
Rodríguez, A. A., Stella, A. M., Storni, M. M., Zulpa, G., & Zaccaro, M. C. (2006). Effects of cyanobacterial extracellular products and gibberellic acid on salinity tolerance in (Oryza sativa L.), Saline systems, 2(1), 1-4.
Rostovtsev, S. A., S A, R., & ES, L. (1978). Determination of the viability of tree and shrub seeds by staining with indigocarmine in the USSR. Seed sci. Technol.; nor; da. 1978, 6(3); 869-875; abs. Fre/ger; bibl. 11 ref.
Stricker G.P., Insaustl A.A., Vega A.S. (2007). Trade – off between root porosity and mechanical strength in species with different types of aerenchyma. Plant Cell Environ, 30, 580-589.
Tran, C. K. (1981). Thuc tap hinh thai va giai phau thuc vat [Practice plant morphology and anatomy]. University and Professional High school Puplisher House, 44-105.
Tran, K. N. (2016). Khao sat anh huong cua stress muoi len su phat trien cua giong Lua OM4900 (Oryza sativa L.) trong giai doan cay con [Investigate the effects of salt stress on the development of rice OM4900 (Oryza sativa L.) varieties in the seedling stage]. Master's Thesis in Biology.
Viet Nam desaster and dyke managemet authoriity. (2020, July 31). Tom tat thien tai tu dau nam 2020 den nay [Summary of natural disasters in Vietnam from the beginning of 2020], Available: http://phongchongthientai.mard.gov.vn/Pages/tom-tat-thien-tai-viet-nam-tu-dau-nam-2020-tinh-den-ngay-23-7-2020-.aspx.
Weber, K. V. (2008), Ecophysiology of high salinity tolerant plants (tasks for vegetation science), 1st edn. Springer, Amsterdam.
Xu, S., Hu, B., He, Z., Ma, F., Feng, J., Shen, W., & Yang, J. (2011). Enhancement of salinity tolerance during rice seed germination by presoaking with hemoglobin. International Journal of Molecular Sciences, 12(4), 2488-2501.
Yildiz, M., Fatih Ozcan, S., T Kahramanogullari, C., & Tuna, E. (2012). The effect of sodium hypochlorite solutions on the viability and in vitro regeneration capacity of the Tissue. The Natural Products Journal, 2(4), 328-331.
Boucau, J., & Ungar, I. A. (1976), Hormonal control of germination under saline conditions of three halophyte taxa in genus Suaeda. Physiol Plant 36: 197–200.
Bui, T. V. (2000). Sinh li thuc vat dai cuong, phan I: dinh duong [Plant physiology, Part I: Nutrition], Ho Chi Minh City National University Puplishing House, 349 pages.
Deinlein, U., Stephan, A. B., Horie, T., Luo, W., Xu, G., & Schroeder, J. I. (2014). Plant salt-tolerance mechanisms. Trends in plant science, 19(6), 371-379.
Hoang, M. T., Nguyen, Q. T. & Vu, Q. S. (2006), Giao trinh Sinh li thuc vat [Plant physiology].
Ha Noi Agricultural Publisher.
Justin, S. H. F. W., & Armstrong, W. (1987). The anatomical characteristics of roots and plant response to soil flooding. New Phytologist, 465-495.
Le, Q. D., Nguyen, T. T., Nguyen, V. N., & Le, K. H. (2011). Tang cuong nang luc va cai bien hat giong va san xuat lua de dam bao an toan luong thuc o vung doi nui cao Viet Nam [Strengthening capacity to improve seed resources and rice production to ensure food security in the high mountains of Vietnam]. Agricultural and Rural Development Publishing House.
Mahajan, S., & Tuteja N., Cold (2005). Salinity and drought stresses: An overview”. Arch, Biochem, Biophys, 444,139-158.
Ministry of Agriculture and Rural Development (2006). Giong va Thoi vu san xuat lua o Dong bang song Cuu Long [Rice varieties and season of rice production in the Mekong Delta] Agriculture Publishing House, 99 pages.
Mizrahi, Y., Blumonfeld, A., Bittner, S., & Richmond, A. E.(1971). Abscisic acid and cytokinin content of leaves in relation to salinity and relative humidity. Plant Physiol, 48, 752-755.
Moore, C. A., Bowen, H. C., Scrase-Field, S., Knight, M. R., & White, P. J. (2002). The deposition of suberin lamellae determines the magnitude of cytosolic Ca2+ elevations in root endodermal cells subjected to cooling. Plant Journal, 30, 457-465.
Mondal, S., Singh, R. P., & Bose, B. (2013). Standardization of Culture Medium for Somatic Embryogenesis of Rice Var. MTU 7029. International journal of Bio-resource and Stress Management, 4(4), 500-505.
Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annu, Rev, Plant Biol., 59, 651-681.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culturé. Physiol Plant, 15, 473-497
National Steering Committee for Natural Disaster Prevention and Control (2020). Retrieved from http://phongchongthientai.mard.gov.vn/Pages/tom-tat-thien-tai-viet-nam-tu-dau-nam-2020-tinh-den-ngay-23-7-2020-.aspx
Negrao, S., Courtois, B., Ahmadi, N., Abreu, I., Saibo, N., & Oliveira, M. M. (2013). Recent updates on salinity stress in rice: From physiological to moleculer responses. Plant Science, 30(4), 329-377.
Nguyen, N. D. (2009). Giao trinh cay lua [Rice cirricular]. Ho Chi Minh City National University Puplishing House.
Ngo, D. T. (2006), Nghien cuu phat trien giong lua chong chiu man cho vung Dong bang song Cuu Long [Research and development of salt-tolerant rice varieties for the Mekong Delta].
Rodríguez, A. A., Stella, A. M., Storni, M. M., Zulpa, G., & Zaccaro, M. C. (2006). Effects of cyanobacterial extracellular products and gibberellic acid on salinity tolerance in (Oryza sativa L.), Saline systems, 2(1), 1-4.
Rostovtsev, S. A., S A, R., & ES, L. (1978). Determination of the viability of tree and shrub seeds by staining with indigocarmine in the USSR. Seed sci. Technol.; nor; da. 1978, 6(3); 869-875; abs. Fre/ger; bibl. 11 ref.
Stricker G.P., Insaustl A.A., Vega A.S. (2007). Trade – off between root porosity and mechanical strength in species with different types of aerenchyma. Plant Cell Environ, 30, 580-589.
Tran, C. K. (1981). Thuc tap hinh thai va giai phau thuc vat [Practice plant morphology and anatomy]. University and Professional High school Puplisher House, 44-105.
Tran, K. N. (2016). Khao sat anh huong cua stress muoi len su phat trien cua giong Lua OM4900 (Oryza sativa L.) trong giai doan cay con [Investigate the effects of salt stress on the development of rice OM4900 (Oryza sativa L.) varieties in the seedling stage]. Master's Thesis in Biology.
Viet Nam desaster and dyke managemet authoriity. (2020, July 31). Tom tat thien tai tu dau nam 2020 den nay [Summary of natural disasters in Vietnam from the beginning of 2020], Available: http://phongchongthientai.mard.gov.vn/Pages/tom-tat-thien-tai-viet-nam-tu-dau-nam-2020-tinh-den-ngay-23-7-2020-.aspx.
Weber, K. V. (2008), Ecophysiology of high salinity tolerant plants (tasks for vegetation science), 1st edn. Springer, Amsterdam.
Xu, S., Hu, B., He, Z., Ma, F., Feng, J., Shen, W., & Yang, J. (2011). Enhancement of salinity tolerance during rice seed germination by presoaking with hemoglobin. International Journal of Molecular Sciences, 12(4), 2488-2501.
Yildiz, M., Fatih Ozcan, S., T Kahramanogullari, C., & Tuna, E. (2012). The effect of sodium hypochlorite solutions on the viability and in vitro regeneration capacity of the Tissue. The Natural Products Journal, 2(4), 328-331.