INVESTIGATION OF THE EFFECT OF NEEM SEED OIL EXTRACT ON THE INHIBITION OF PLUTELLA XYLOSTELLA L. ON BRASSICA JUNCEA IN TIEN GIANG PROVINCE

Hoàng Dũng Nguyễn , Thị Mỹ Hạnh Trần , Lương Hiếu Hòa Nguyễn , Thị Phương Dung Ngô , Thị Duyên Lương , Quốc Khang Nguyễn

Main Article Content

Abstract

 

The research and application of herbs derived plant protection drugs is the trend to replace chemical drugs in cultivation towards organic agriculture. In this study, essential oil containing azadirachtin was extracted from neem seeds and was tested for its effectiveness in killing silkworms on green mustard (Brassica juncea). The results showed that neem seed oil had an insecticidal positive effect, proportional to the treatment concentration. The 70% inhibitory concentration of silkworms (LC70) reached 0.0250 %. In the greenhouse, the first treatment of neem oil product was efficient at 33.33% in controlling silkworms. By the 7th day after the second treatment, the insecticide efficiency reached 72.5%. The obtained results show that the high application potential of neem oil in vegetable cultivation is to limit the use of chemical drugs, as well as the status of resistant pests.

 

Article Details

References

Aggarwal, N., Holaschke, M., Basedow, T. (2006). Evaluation of biorational insecticides to control Helicoverpa armigera (Hübner) and Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) fed on Vicia faba L. Journal of Applied Entomology, 15 : 245-250.
Asogwa, E. U., Ndubuaku, T. C. N., Ugwu, J. A., & Awe, O. O. (2010). Prospects of botanical pesticides from neem, Azadirachta indica for routine protection of cocoa farms against the brown cocoa mirid-Sahlbergella singularis in Nigeria. J. Med. Plants Res, 4 (1), 1-6.
Darabian, K., & Yarahmadi, F. (2017). Field Efficacy of Azadirachtin, Chlorfenapyr, and Bacillus thuringensis against Spodoptera exigua (Lepidoptera: Noctuidae) on Sugar Beet Cropt,
J. Entomol. Res. Soc., 19(3), 45-52.
Fei Yi, Chunhua Zou, Qiongbo Hu, & Meiying Hu (2012). The joint action of destruxins and botanical insecticides against the cotton aphid, Aphis gossypii glover. Molecules 17, 7533-7542.
Grainge, M., Ahmed, S., Mitchell, W. C., & Hylin J. W. (1984). Plant species reportedly possessing pest control properties, a database, Resource systems, Institute East-west Conter, Honolulu, Hawaii, USA.
Jacobson, M. (1990), In natural resistance of plants to pests: Roles of Allelo chemicals, ACS Symposium series No. 296, American chemical society, Washington DC, 220-232.
Konecka, E., Kaznowski, A., & Tomkowiak, D. (2019). Insecticidal activity of mixtures of Bacillus thuringiensis crystals with plant oils of Sinapis alba and Azadirachta indica. Ann. Appl. Biol. 174, 364-371. doi: 10.1111/aab.12502
Mhazo, M. L., Mhazo, N., Michael, T., & Masarirambi, M. T. (2011). The effectiveness of home made organic pesticides derived from wild plants (Solanum pindiriforme and Lippia javanica), garlic (Allium sativum) and tobacco (Nicotiana tobacum) on aphid (Brevicoryne brassica) mortality on rape (Brassica napus) plants. Res. J. Environ. Earth Sci. 3(5), 457-462.
Nguyen, T. Q. (2005). Nghien cuu anh huong cua dau neem len su ky sinh va phat trien cua bo ha trong cu va tren ruong khoai lang – Hoi nghi cac bien phap sinh hoc trong phong chong sau benh hai tren cay trong nong nghiep toan quoc [Effect of the neem oil on the weevil aldult (Cylas formicariu L.) parasitism in sweet potato tubes and filed - Froceeding of national conference on biological measures in the prevention of plant pests and diseases in agriculture.
Satti, A. A., Ellaithy, M. E., & Mohamed, A. E. (2010). Insecticidal activities of neem (Azadirachta indica A. Juss) seeds under laboratory and field conditions as affected by different storage durations. Agric. Biol. J. N. Am., 1(5), 1001-1008.
Shu, B., Yu, H., Li, Y., Zhong, H., Li, X., Cao, L., Lin, J. (2020). Identification of azadirachtin responsive genes in Spodoptera frugiperda larvae based on RNA-seq. Pest. Biochem. Physiol. 172, 104745. doi: 10.1016/j.pestbp.2020.104745
Shu, B., Zhang, J., Cui, G., Sun, R., Yi, X., & Zhong, G. (2018). Azadirachtin Affects the Growth of Spodoptera litura Fabricius by Inducing Apoptosis in Larval Midgut. Frontiers in physiology, 9, 137. https://doi.org/10.3389/fphys.2018.00137.
Vinuela, E., Adan, A., Smagghe, G., Gonzalez, M., Medina, M. P., Budia, F., . . . Estal, P. D. (2000). Laboratory Effects of Ingestion of Azadirachtin by Two Pests (Ceratitis capitata and Spodoptera exigua) and Three Natural Enemies (Chrysoperla carnea, Opius concolor and Podisus maculiventris). Biocontrol Science and Technology, 10(2), 165-177.
Vo, V. K. (2005). Nghien cuu su dung cac thanh phan cay Neem lam thuoc bao ve thuc vat – Bao cao hoi nghi sinh hoc trong phong chong sau benh hai cay trong nong nghiep toan quoc [Investiage the components of Neem tree as the biological plant protection agents – Proceeding of the National Conference on biological measures in the control of agricultural crop pests and disease]
Wandscheer, C. B., Duque, J. E., da Silva, M. A., Fukuyama, Y., Wohlke, J. L., Adelmann, J., & Fontana, J. D. (2004). Larvicidal action of ethanolic extracts from fruit endocarps of Melia azedarach and Azadirachta indica against the dengue mosquito Aedes aegypti. Toxicon:official journal of the International Society on Toxinology, 44(8), 829-835. https://doi.org/10.1016/j.toxicon.2004.07.009