SEMI-CONTINUOUS CULTIVATION OF THE GREEN STAGE BIOMASS OF Haematococcus pluvialis IN NEW ANGLED PSBRs
Main Article Content
Abstract
Besides suspension cultivation, immobilized cultivation in small-scale angled PSBRs has been developed recently to proliferate the green stage biomass of Haematococcus pluvialis for large-scale PSBRs. In this study, H. pluvialis biofilms were cultured semi-continuously in PSBRs to increase the biomass of the vegetative stage. Renewal of the nutrient medium at a ratio of 30% (equivalent to 1.5 L) per day resulted in an increase in algal biomass of 76 g.m-2 after 20 days of semi-continuous cultivation. The harvesting interval of 10 days apart and the algae biomass collection ratio of 90% achieved the highest efficiency. The total harvested biomass of algae at the vegetative stage was 104 g.m-2 after 20 days of semi-continuous immobilized cultivation.
Keywords
astaxanthin, chlorophyll, green stage, H. pluvialis, PSBR, semi-continuous
Article Details
References
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Fabregas, J., Otero, A., Maseda, A., & Dominguez, A. (2001). Two-stage cultures for the production of astaxanthin from Haematococcus pluvialis. J Biotechnol, 89(1), 65-71. https://doi.org/10.1016/s0168-1656(01)00289-9
Imamoglu, E., Dalay, M. C., & Sukan, F. V. (2010). Semi-continuous cultivation of Haematococcus pluvialis for commercial production. Applied Biochemistry and Biotechnology, 160(3), 764-772. https://doi.org/10.1007/s12010-009-8627-7
Khoo, K. S., Lee, S. Y., Ooi, C. W., Fu, X., Miao, X., Ling, T. C., & Show, P. L. (2019). Recent advances in biorefinery of astaxanthin from Haematococcus pluvialis. Bioresource Technology, 288, Article 121606. https://doi.org/10.1016/j.biortech.2019.121606
Kiperstok, A. C., Melkonian, M., & Becker, B. (2016). Optimizing immobilized cultivation of Haematococcus pluvialis for astaxanthin production [Ph.D. Thesis, Universität zu Köln]. http://kups.ub.uni-koeln.de/id/eprint/6728
Kiperstok, A. C., Sebestyén, P., Podola, B., & Melkonian, M. (2017). Biofilm cultivation of Haematococcus pluvialis enables a highly productive one-phase process for astaxanthin production using high light intensities. Algal Research, 21, 213-222. https://doi.org/10.1016/j.algal.2016.10.025
Kobayashi, M., Kurimura, Y., Kakizono, T., Nishio, N., & Tsuji, Y. (1997). Morphological changes in the life cycle of the green alga Haematococcus pluvialis. Journal of Fermentation and Bioengineering, 84(1), 94-97. https://doi.org/10.1016/S0922-338X(97)82794-8
Li, F., Cai, M., Lin, M., Huang, X., Wang, J., Ke, H., Wang, C., Zheng, X., Chen, D., & Yang, S. (2020). Enhanced Biomass and Astaxanthin Production of Haematococcus pluvialis by a Cell Transformation Strategy with Optimized Initial Biomass Density. Marine Drugs, 18(7), Article 341. https://doi.org/10.3390/md18070341
Li, J., Zhu, D., Niu, J., Shen, S., & Wang, G. (2011). An economic assessment of astaxanthin production by large scale cultivation of Haematococcus pluvialis. Biotechnology Advances, 29(6), 568–574. https://doi.org/10.1016/j.biotechadv.2011.04.001
Li, T., Podola, B., Schultze, L. K. P., & Melkonian, M. (2019). Design scenario analysis for porous substrate photobioreactor assemblies. Journal of Applied Phycology, 31(3), 1623-1636. https://doi.org/10.1007/s10811-018-1700-2
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Mantzorou, A., & Ververidis, F. (2019). Microalgal biofilms: A further step over current microalgal cultivation techniques. Science of The Total Environment, 651, 3187-3201. https://doi.org/10.1016/j.scitotenv.2018.09.355
Oslan, S. N. H., Shoparwe, N. F., Yusoff, A. H., Abdul Rahim, A., Chang, C. S., Tan, J. S., Oslan, S. N., Arumugam, K., Ariff, A. B., Sulaiman, A. Z., & Mohamed, M. S. (2021). A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin. Biomolecules, 11(2), Article 256. https://doi.org/10.3390/biom11020256
Panis, G., & Carreon, J. R. (2016). Commercial astaxanthin production derived by green alga Haematococcus pluvialis: A microalgae process model and a techno-economic assessment all through production line. Algal Research, 18, 175-190. https://doi.org/10.1016/j.algal.2016.06.007
Shah, M. M., Liang, Y., Cheng, J. J., & Daroch, M. (2016). Astaxanthin-producing green microalga Haematococcus pluvialis: from single cell to high value commercial products. Front Plant Sci, 7, Article 531. https://doi.org/10.3389/fpls.2016.00531
Tran, H. D., Ong, B. N., Ngo, V. T., Tran, D. L., Nguyen, T. C., Tran, T. B. H., Do, T. T., Nguyen, T. M. L., Nguyen, X. H., & Melkonian, M. (2022). New Angled Twin–Layer Porous Substrate Photobioreactors for Cultivation of Nannochloropsis oculata. Protist, Article 125914. https://doi.org/10.1016/J.PROTIS.2022.125914
Wen, X., Wang, Z., Ding, Y., Geng, Y., & Li, Y. (2020). Enhancing the production of astaxanthin by mixotrophic cultivation of Haematococcus pluvialis in open raceway ponds. Aquaculture International, 28(2), 625-638. https://doi.org/10.1007/S10499-019-00483-2/TABLES/3
Wu, K., Ying, K., Zhou, J., Hanotu, J., Zhu, X., & Cai, Z. (2021). Optimizing the growth of Haematococcus pluvialis based on a novel microbubble-driven photobioreactor. Iscience, 24, Article 103461. https://doi.org/10.1016/j.isci.2021.103461
Zhang, B. Y., Geng, Y. H., Li, Z. K., Hu, H. J., & Li, Y. G. (2009). Production of astaxanthin from Haematococcus in open pond by two-stage growth one-step process. Aquaculture, 295(3), 275-281. https://doi.org/10.1016/j.aquaculture.2009.06.043
Zhang, W., Wang, J., Wang, J., & Liu, T. (2014). Attached cultivation of Haematococcus pluvialis for astaxanthin production. Bioresour Technol, 158, 329-335. https://doi.org/10.1016/j.biortech.2014.02.044
Do, T.-T., Tran-Thi, B.-H., Ong, B.-N., Le, T.-L., Nguyen, T.-C., Quan, Q.-D., Le, T.-C., Tran, D.-L., Melkonian, M., & Tran, H.-D. (2021). Effects of red and blue light emitting diodes on biomass and astaxanthin of Haematococcus pluvialis in pilot scale angled twin-layer porous substrate photobioreactors. Vietnam Journal of Science, Technology and Engineering, 63(2), 81-88. https://doi.org/10.31276/VJSTE.63(2).81-88.81-88)
Fabregas, J., Otero, A., Maseda, A., & Dominguez, A. (2001). Two-stage cultures for the production of astaxanthin from Haematococcus pluvialis. J Biotechnol, 89(1), 65-71. https://doi.org/10.1016/s0168-1656(01)00289-9
Imamoglu, E., Dalay, M. C., & Sukan, F. V. (2010). Semi-continuous cultivation of Haematococcus pluvialis for commercial production. Applied Biochemistry and Biotechnology, 160(3), 764-772. https://doi.org/10.1007/s12010-009-8627-7
Khoo, K. S., Lee, S. Y., Ooi, C. W., Fu, X., Miao, X., Ling, T. C., & Show, P. L. (2019). Recent advances in biorefinery of astaxanthin from Haematococcus pluvialis. Bioresource Technology, 288, Article 121606. https://doi.org/10.1016/j.biortech.2019.121606
Kiperstok, A. C., Melkonian, M., & Becker, B. (2016). Optimizing immobilized cultivation of Haematococcus pluvialis for astaxanthin production [Ph.D. Thesis, Universität zu Köln]. http://kups.ub.uni-koeln.de/id/eprint/6728
Kiperstok, A. C., Sebestyén, P., Podola, B., & Melkonian, M. (2017). Biofilm cultivation of Haematococcus pluvialis enables a highly productive one-phase process for astaxanthin production using high light intensities. Algal Research, 21, 213-222. https://doi.org/10.1016/j.algal.2016.10.025
Kobayashi, M., Kurimura, Y., Kakizono, T., Nishio, N., & Tsuji, Y. (1997). Morphological changes in the life cycle of the green alga Haematococcus pluvialis. Journal of Fermentation and Bioengineering, 84(1), 94-97. https://doi.org/10.1016/S0922-338X(97)82794-8
Li, F., Cai, M., Lin, M., Huang, X., Wang, J., Ke, H., Wang, C., Zheng, X., Chen, D., & Yang, S. (2020). Enhanced Biomass and Astaxanthin Production of Haematococcus pluvialis by a Cell Transformation Strategy with Optimized Initial Biomass Density. Marine Drugs, 18(7), Article 341. https://doi.org/10.3390/md18070341
Li, J., Zhu, D., Niu, J., Shen, S., & Wang, G. (2011). An economic assessment of astaxanthin production by large scale cultivation of Haematococcus pluvialis. Biotechnology Advances, 29(6), 568–574. https://doi.org/10.1016/j.biotechadv.2011.04.001
Li, T., Podola, B., Schultze, L. K. P., & Melkonian, M. (2019). Design scenario analysis for porous substrate photobioreactor assemblies. Journal of Applied Phycology, 31(3), 1623-1636. https://doi.org/10.1007/s10811-018-1700-2
Lorenz, R. T., & Cysewski, G. R. (2000). Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Trends in Biotechnology, 18(4), 160-167. https://doi.org/10.1016/S0167-7799(00)01433-5
Mantzorou, A., & Ververidis, F. (2019). Microalgal biofilms: A further step over current microalgal cultivation techniques. Science of The Total Environment, 651, 3187-3201. https://doi.org/10.1016/j.scitotenv.2018.09.355
Oslan, S. N. H., Shoparwe, N. F., Yusoff, A. H., Abdul Rahim, A., Chang, C. S., Tan, J. S., Oslan, S. N., Arumugam, K., Ariff, A. B., Sulaiman, A. Z., & Mohamed, M. S. (2021). A Review on Haematococcus pluvialis Bioprocess Optimization of Green and Red Stage Culture Conditions for the Production of Natural Astaxanthin. Biomolecules, 11(2), Article 256. https://doi.org/10.3390/biom11020256
Panis, G., & Carreon, J. R. (2016). Commercial astaxanthin production derived by green alga Haematococcus pluvialis: A microalgae process model and a techno-economic assessment all through production line. Algal Research, 18, 175-190. https://doi.org/10.1016/j.algal.2016.06.007
Shah, M. M., Liang, Y., Cheng, J. J., & Daroch, M. (2016). Astaxanthin-producing green microalga Haematococcus pluvialis: from single cell to high value commercial products. Front Plant Sci, 7, Article 531. https://doi.org/10.3389/fpls.2016.00531
Tran, H. D., Ong, B. N., Ngo, V. T., Tran, D. L., Nguyen, T. C., Tran, T. B. H., Do, T. T., Nguyen, T. M. L., Nguyen, X. H., & Melkonian, M. (2022). New Angled Twin–Layer Porous Substrate Photobioreactors for Cultivation of Nannochloropsis oculata. Protist, Article 125914. https://doi.org/10.1016/J.PROTIS.2022.125914
Wen, X., Wang, Z., Ding, Y., Geng, Y., & Li, Y. (2020). Enhancing the production of astaxanthin by mixotrophic cultivation of Haematococcus pluvialis in open raceway ponds. Aquaculture International, 28(2), 625-638. https://doi.org/10.1007/S10499-019-00483-2/TABLES/3
Wu, K., Ying, K., Zhou, J., Hanotu, J., Zhu, X., & Cai, Z. (2021). Optimizing the growth of Haematococcus pluvialis based on a novel microbubble-driven photobioreactor. Iscience, 24, Article 103461. https://doi.org/10.1016/j.isci.2021.103461
Zhang, B. Y., Geng, Y. H., Li, Z. K., Hu, H. J., & Li, Y. G. (2009). Production of astaxanthin from Haematococcus in open pond by two-stage growth one-step process. Aquaculture, 295(3), 275-281. https://doi.org/10.1016/j.aquaculture.2009.06.043
Zhang, W., Wang, J., Wang, J., & Liu, T. (2014). Attached cultivation of Haematococcus pluvialis for astaxanthin production. Bioresour Technol, 158, 329-335. https://doi.org/10.1016/j.biortech.2014.02.044