ISOLATION, SELECTION OF FUNGUS STRAINS CAPABLE OF PARASITICALLY KILLING COCONUT BLACKHEAD WORMS (Opisina arenosella Walker) IN BEN TRE PROVINCE
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Abstract
Blackhead worm (Opisina arenosella Walker) is one of the species that seriously affects coconut productivity in Ben Tre province. In this article, we isolated, screened, and selected fungal strains capable of parasitizing harmful blackhead worms on coconut trees in Ben Tre province. From 40 worm samples, 25 soil samples, and 25 leaf samples collected in Ben Tre and An Giang provinces, 147 fungal strains were isolated, and 25 fungal strains were screened for their ability to simultaneously secrete the enzyme chitinase and protease, demonstrating the ability to degrade chitin and proteins in the worm's cuticle layer. Among them, Metarhizium anisopliae S39.6 and Talaromyces pinophilus D6.6 can kill black-headed worms with an effectiveness of 100% and 85.77%, respectively, after 7 days of treatment. These two fungal strains have the potential for the prevention and treatment of harmful blackhead worms on coconut trees in Ben Tre province.
Keywords
Black Headed Caterpillar, chitinase, fungi, protease
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References
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