A STUDY ON THE EFFECTS OF LIGHTING TIME AND CULTURED MEDIUM ON GERMINATION OF Tribulus terrestris L. SEEDS IN VITRO
Main Article Content
Abstract
Tribulus terrestris L. is a rare medicinal herb, listed in the IUCN Red List of Threatened Species and ranked EN in the Vietnam Red Book. In this study, we evaluated the germination of Tribulus terrestris seeds under different lighting conditions and cultured medium. Research results show that the cultured environments with ½ MS or ½ NH4NO3 have the highest survival rate, reaching 93% in about four days. When combining ½ MS medium and 20-hour lighting time, 97% of tribulus terrestris seeds are stable, and the incubation time is three days. On the contrary, in a medium with sugar content up to 90 g/L, Tribulus terrestris seeds cannot germinate.
Keywords
cultured medium, germination, in vitro, lighting time, Tribulus terrestris L.
Article Details
References
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Zeng, S., Wu, K., da Silva, J. A. T., Zhang, J., Chen, Z., Xia, N., & Duan, J. (2012). Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid. Scientia Horticulturae, 138, 198-209. http://dx.doi.org/10.1016/j.scienta.2012.02.026
Zhang, J. D., Xu, Z., Cao, Y. B., Chen, H. S., Yan, L., An, M. M., Gao, P. H., Wang, Z., Jia, X. M., & Jiang, Y. Y. (2006). Antifungal activities and action mechanisms of compounds from Tribulus terrestris L. Journal of ethnopharmacology, 103(1), 76-84. https://doi.org/10.1016/j.jep.2005.07.006
Chu, V. M. (2009). Tin học trong cong nghe sinh hoc [Informatics in biotechnology]. Vietnam Educational Publishing House.
Do, T. L. (2004). Nhung cay thuoc và vi thuoc Viet Nam [Vietnamese medicinal plants and herbs]. Medical Publishing House.
Hu, K., & Yao, X. (2002). Protodioscin (NSC-698 796): its spectrum of cytotoxicity against sixty human cancer cell lines in an anticancer drug screen panel. Planta medica, 68(04), 297-301.
Joel, F., Claudia, G-S., & Enrique, J. (2016). Effect of light on seed germination and seedling shape of succulent species from Mexico. Journal of Plant Ecology, 9, 174-179. http://doi.org/10.1093/jpe/rtv046
Khuong, T. T. H., Le, T. V. A. & Tran, K. V. (2018). Giao trinh sinh ly thuc vat [Plant Physiology Textbook]. Science and Technology Publishing House.
Le, N. T. N., Tran, T. T. T., Hoang, T. M. N., Vo, N. T. A., Tran, T. T. L., & Quach, V. T. E. (2022). Nghien cuu anh huong của chat dieu hoa tang truong thuc vat IBA and BA den kha nang nay mam cua hat Gai ma vuong (Tribulus terrertris L.) trong dieu kien in vitro [Study on the effects of growth regulators IBA and BA on the germination ability of Tribulus terrertris L. seeds in vitro]. Ho Chi Minh City University of Education Journal of Science, 19(12), 2076-2089. https://doi.org/10.54607/hcmue.js.19.12.3558(2022)
Li, X., Huang, C., Song, Q., Zhou, J., Wang, X., Qu, T., Ting, T., Huang, F., & Bu, Z. (2016). In vitro asymbiotic germination and propagation of Paphiopedilum concolor (Lindl.) Pfitz. Plant Science Journal, 34(1), 127-134.
Masheva, S., Yankova, V., Markova, D., Boteva, H., & Dincheva, T. (2011). Diseases and pests on Tribulus Terrestris L.-wild growing plant and semi-crop. Biotechnology & Biotechnological Equipment, 25(2), 2391-2393. http://doi.org/10.5504/BBEQ.2011.0042
Ministry of Science and Technology. (2007). Sach Do Viet Nam [Vietnam's Red Data Book]. Publishing House for Science and Technology.
Pandey, R., Shankar, B. S., & Sainis, K. B. (2007). Tribulus terrestris. Fruit Extract Protects Against Oxidative Stress-Induced Apoptosis. Pharmaceutical Biology, 45(8), 619-625.
Perrone, A., Plaza, A., Bloise, E., Nigro, P., Hamed, A. I., Belisario, M. A., Pizza, C., & Piacente, S. (2005). Cytotoxic Furostanol Saponins and a Megastigmane Glucoside from Tribulus p arvispinus. Journal of natural products, 68(10), 1549-1553. https://doi.org/10.1021/np0502138
Phi, T. C. M. (2012). Nghien cuu nhan nhanh in vitro loai Lan Kim tuyen (Anoectochilus setaceus blume) nham bao ton nguon duoc lieu quy [Research on rapid in vitro multiplication of Anoectochilus setaceus blume to preserve precious medicinal resources]. [MSc Thesis: Biology, University of Natural Sciences, Ha Noi National University].
Phillips, O. A., Mathew, K. T., & Oriowo, M. A. (2006). Antihypertensive and vasodilator effects of methanolic and aqueous extracts of Tribulus terrestris in rats. Journal of ethnopharmacology, 104(3), 351-355. https://doi.org/10.1016/j.jep.2005.09.027
Roland, C. (2020). Tribulus terrestris. The IUCN Red List of Threatened Species, 2020, Article e.T203497A84011807. https://doi.org/10.2305/IUCN.UK.2020-3.RLTS.T203497A84011807.en
Semerdjieva, I. B., & Zheljazkov, V. D. (2019). Chemical constituents, biological properties, and uses of Tribulus terrestris: A Review. Natural Product Communications, 14(8). https://doi.org/10.1177/1934578X19868394
Tarmizi, N. H. A., Ja’afar, N. M., Dolhaji, N. H., & Latif, P. A. (2023). Effect of Different Sucrose Concentration on In Vitro Germination of Rice (Oryza sativa L.) cv. MR269. International Journal of Advanced Agriculture and Food Technology, 4(1), Article 46. https://amcs-press.com/index.php/ijafat/article/view/46
Vu, H. S., Nguyen, T. X., Nguyen, T. H., Duong, T. N. A., Nguyen, M. T., Nguyen, D. P.,
& Phan, T. H. (2020). Nghien cuu qua trinh tao cu cua Rehmannia glutinosa (Gaertn.) Libosch [Research on the tuber formation process of Rehmannia glutinosa (Gaertn.) Libosch]. Vietnam Journal of Biotechnology, 18(3), 507-516. https://doi.org/10.15625/1811-4989/18/3/15713
Vu, V. L. (2007). San xuat giong và cong nghe hat giong [Seed production and seed technology]. University of Agriculture.
Zeng, S., Wu, K., da Silva, J. A. T., Zhang, J., Chen, Z., Xia, N., & Duan, J. (2012). Asymbiotic seed germination, seedling development and reintroduction of Paphiopedilum wardii Sumerh., an endangered terrestrial orchid. Scientia Horticulturae, 138, 198-209. http://dx.doi.org/10.1016/j.scienta.2012.02.026
Zhang, J. D., Xu, Z., Cao, Y. B., Chen, H. S., Yan, L., An, M. M., Gao, P. H., Wang, Z., Jia, X. M., & Jiang, Y. Y. (2006). Antifungal activities and action mechanisms of compounds from Tribulus terrestris L. Journal of ethnopharmacology, 103(1), 76-84. https://doi.org/10.1016/j.jep.2005.07.006