KHẢO SÁT HOẠT TÍNH KHÁNG OXY HÓA VÀ KHÁNG UNG THƯ CỦA CÀNH BẰNG LĂNG NƯỚC Lagerstroemia speciosa (L.) Per

Abstract

Lagerstroemia speciosa is a plant widely grown in Vietnam. However, there are still not many studies on the biological activities of this species. The purpose of this research is to assess the antioxidant and anticancer activities of Lagerstroemia speciosa branches (collected in An Giang). Dried powder of Lagerstroemia speciosa branches was extracted with the ethanolic solvents, and then liquid-liquid extraction obtained n-hexane, chloroform, ethyl acetate, and water fractions, respectively. The results show that the total phenolic content in the ethyl acetate and water fractions was the highest with values of (289.93 ± 4.14) and (279.61 ± 6.36) µg GAE/mg extract. The efficiency of hydrogen-free radical scavenging at DPPH of the aqueous fraction (EC₅₀ = 40.74 µg/mL) was higher than that of the ethyl acetate, chloroform, and n-hexane fractions with EC₅₀ values of (79.52, 156.22, ≥ 500) µg/mL, respectively. Through the MTT method, the fractionated extracts also show a strong cytotoxic activity on HepG2 liver cancer cells, A549 lung cancer, and B16F10 skin cancer cells, except that the aqueous extracts were outside the investigated concentrations (IC₅₀ >200 µg/mL). The n-hexane extract shows good cytotoxicity on the B16F10 cell line with an IC₅₀ of about 71.18 µg/mL while the chloroform fraction, ethyl acetate exhibits good cytotoxic activity on A549 cells with a value IC₅₀ being 60.71 and 75.49 µg/mL, respectively.