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Tiếng Viêth
Date Published: 23/01/2025
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Views Tiếng Viêth: 112
DOI: https://doi.org/10.54607/hcmue.js.22.1.4151(2025)
Issue
Vol. 22 No. 1 (2025)
Section
Articles
How to Cite
Trần , T. M. Đ., Lê , T. P., & Nguyễn , T. H. (2025). ISOLATION OF ENTEROBACTERIACEAE FROM A POPIAH SAMPLE COLLECTED IN HO CHI MINH CITY AND ASSESSMENT OF THEIR ANTIBIOTIC RESISTANCE. HCMUE Journal of Science, 22(1), 15-26. https://doi.org/10.54607/hcmue.js.22.1.4151(2025)

ISOLATION OF ENTEROBACTERIACEAE FROM A POPIAH SAMPLE COLLECTED IN HO CHI MINH CITY AND ASSESSMENT OF THEIR ANTIBIOTIC RESISTANCE

Thị Minh Định Trần 1, , Thảo Phương Lê 2, Thị Hương Nguyễn 2
1 Khoa Sinh học Trường Đại học Sư phạm Thành phố Hồ Chí Minh
2 Khoa Sinh học, Trường Đại học Sư phạm Thành phố Hồ Chí Minh

Main Article Content

Abstract

Members of the Enterobacteriaceae family are commonly used as indicators of food hygiene and include several important foodborne pathogens. This study aimed to isolate Enterobacteriaceae from a popiah sample collected in Ho Chi Minh City and investigate their antibiotic resistance status. Bacterial isolation was performed using Endo agar and MacConkey agar, yielding 180 strains. Identification was conducted through six biochemical tests (indole, methyl red, Voges-Proskauer, citrate utilization, motility, and Triple Sugar Iron) and reference to Bergey's Manual (2005), confirming that 25 of 180 strains (13.89%) belonged to the Enterobacteriaceae family. Serratia marcescens was the most prevalent species (2.78%), followed by Hafnia (2.22%), Pseudomonas aeruginosa (1.67%), Klebsiella pneumoniae (1.67%), Klebsiella oxytoca (1.67%), Salmonella Typhi (1.11%), Edwardsiella tarda (0.56%), Enterobacter cloacae (0.56%), Proteus myxofaciens (0.56%), Morganelia morganii (0.56%), and Yersinia pestis (0.56%). Antibiotic susceptibility testing revealed high resistance to ampicillin (76%), moderate resistance to gentamicin (8%), and no resistance to ciprofloxacin (0%). These findings suggest that the popiah sample was relatively contaminated with Enterobacteriaceae bacteria, posing a potential risk of infection to consumers.

Keywords

Antibiotic resistance, Enterobacteriaceae, food hygiene, popiah

Article Details

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