EFFECTS OF HERBAL EXTRACT COMBINATION SOLUTION ON PHYSIOLOGICAL AND HEPATIC FUNCTION IN ALBINO MOUSE (Mus musculus var. albino)
Main Article Content
Abstract
Nowadays, screening strategies for therapies targeting hepatic injury are becoming increasingly popular, with a growing body of research focusing on herbal medicine. Studies have demonstrated that Scutellaria barbata, Hedyotis diffusa, and Celastrus hindsii possess the potential to treat liver diseases. However, the biological activity of a combination of these three herbs remains unclear. This study aimed to evaluate the hepatoprotective and regenerative effects of a mixed herbal decoction in an albino mouse model. Mice were intraperitoneally injected with carbon tetrachloride (CCl4) to induce hepatic injury. The decoction was administered both before and after CCl₄ injection. Hematological parameters such as hemoglobin, hematocrit, and liver enzymes (AST and ALT) are examined. At 72 hours post-injection, pre-treatment with the decoction at a concentration of 125 μg/mL exhibited the most significant hepatoprotective effects. This was evidenced by an increase in hematological parameters and a marked decrease in liver enzyme levels compared to the negative control group. The combined decoction shows potential for supporting hepatic injury treatment.
Article Details
References
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Huo, H. Z., Wang, B., Liang, Y. K., Bao, Y. Y., & Gu, Y. (2011). Hepatoprotective and antioxidant effects of licorice extract against CCl4-induced oxidative damage in rats. International Journal of Molecular Sciences, 12(10), 6529-6543. https://doi.org/10.3390/ijms12106529
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Ly, T. N., Shimoyamada, M., & Yamauchi, R. (2006). Isolation and characterization of rosmarinic acid oligomers in Celastrus hindsii Benth leaves and their antioxidative activity. Journal of Agricultural and Food Chemistry, 54(11), 3786-3793. https://doi.org/10.1021/jf052743f
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Maisetta, G., Batoni, G., Caboni, P., Esin, S., Rinaldi, A. C., & Zucca, P. (2019). Tannin profile, antioxidant properties, and antimicrobial activity of extracts from two Mediterranean species of parasitic plant Cytinus. BMC Complementary and Alternative Medicine, 19(1), Article 82. https://doi.org/10.1186/s12906-019-2487-7
Martínez-Calva, I., Campos-Apáez, A., Rosales-Vega, E., & Mourelle, M. (1984). Vitamin E improves membrane lipid alterations induced by CCl4 intoxication. Journal of Applied Toxicology, 4(5), 270-272. https://doi.org/10.1002/jat.2550040513
Nemzek, J. A., Bolgos, G. L., Williams, B. A., & Remick, D. G. (2001). Differences in normal values for murine white blood cell counts and other hematological parameters based on sampling site. Inflammation research, 50(10), 523-527. https://doi.org/10.1007/PL00000229
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Nguyen, T. T. H., & Vo, V. T. (2019). Laboratory practice human and animal physiology [Thuc hanh sinh li hoc nguoi va dong vat]. Ho Chi Minh City University of Education Publisher.
O'Connell, K. E., Mikkola, A. M., Stepanek, A. M., Vernet, A., Hall, C. D., Sun, C. C., Yildirim, E., Staropoli, J. F., Lee, J. T., & Brown, D. E. (2015). Practical murine hematopathology: A comparative review and implications for research. Comparative medicine, 65(2), 96-113. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408895/#:~:text=Mice%20in%20health%20have%20many,marrow%20lymphocyte%20percentages%2C%20variable%20leukocyte
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Pham, D. C., Nguyen, H. C., Nguyen, T. L., Ho, H. L., Trinh, T. K., Riyaphan, J., & Weng, C. F. (2020). Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities. Biomed Research International, 2020, Article 3497107. https://doi.org/10.1155/2020/3497107
Phan, K. D., Vo, T. M. H., Trinh, D. H. M., Le, T. D., Chan, C. L., Nguyen, T. T., & Dai, T. X. T. (2020). Nghien cuu mot so hoat tinh sinh hoc cua cao chiet methanol cay luoi ran trang (Hedyotis diffusa willd.). Can Tho University Journal of Science, 56, 103-114. https://doi.org/10.22144/ctu.jsi.2020.118
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Scholten, D., Trebicka, J., Liedtke, C., & Weiskirchen, R. (2015). The carbon tetrachloride model in mice. Laboratory Animals, 49(51), 4-11. https://doi.org/10.1177/0023677215571192
Tran, D. V., Chan, D. X., & La, H. A. (2021). α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials. Molecules, 26(23), Article 7248. https://doi.org/10.3390/molecules26237248
Tran, T. L. G., Nguyen, T. N., Nguyen, K. D., Le, N. T. L., Trinh, T. B., Nguyen, P. A. U, & Bui, D. T. (2017). Antioxidant properties of flavonoids extracted from some vietnamese medicinal plants. European Journal of Advanced Research in Biological and Life Sciences, 5(1), 1-8. https://www.idpublications.org/wp-content/uploads/2016/12/Full-Paper-antioxidant-properties-of-flavonoids-extracted-from-some-vietnamese.pdf
Tran, T. Y. N. (2022). Hoat tinh khang oxy hoa cua cao chiet cay Hy thiem (Siegesbeckia orientalis L.) [Evaluation of the antioxidant activity of methanol extracts of Siegesbeckia orientalis L.]. Thu Dau Mot University Journal of Science, 3(58), 69-78. https://doi.org/10.37550/tdmu.VJS/2022.03.308
World Health Organization. (2019). Viet Nam. https://data.who.int/countries/704
Yan, C., Kong, F., & Ou, X. (2012). Antioxidant and anti-glycated activities of polysaccharides in vitro isolated from Hedyotis diffusa Willd. Journal of Medicinal Plants Research, 6(14), 2895-2900. https://doi.org/10.5897/JMPR11.1769
Alm-Eldeen, A. A., El-Naggar, S. A., El-Boray, K. F., Elgebaly, H. A., & Osman, I. H. (2015). Protective Role of Commiphora molmol Extract against Liver and Kidney Toxicity Induced by Carbon Tetrachloride in Mice. Tropical Journal of Pharmaceutical Research, 15(1), 65-72. https://doi.org/10.4314/tjpr.v15i1.9
Asmaa., Madthi, S., Al-Diwan, M. A., & Al-Jadaan, S. A. N. (2018). Hematological profile of rats treated with quercetin derivative against carbon tetrachloride (CCL4) toxicity. Basrah Journal of Veterinary Research, 17(2), 70-84. https://doi.org/10.13140/RG.2.2.25397.32483
Bui, T. T. D., Dang, K. T., Vu, M. H., & Bui, T. T. (2020). Nghien cuu tac dung uc che te bao ung thu va chong oxy hoa cua la xa den (Celastrus hindsii Benth et Hook.). VNU Journal of Science: Medical and Pharmaceutical Sciences, 36, 39-45. https://doi.org/10.25073/2588-1132/vnumps.4203
Cui, X., Gu, X., & Kang, W. (2016). Antioxidant activity in vitro and hepatoprotective effects in vivo of compound Lobelia. African journal of traditional, complementary, and alternative medicines : AJTCAM, 13(5), 114-122. https://doi.org/10.21010/ajtcam.v13i5.15
Chen, R., He, J., Tong, X., Tang, L., & Liu, M. (2016). The Hedyotis diffusa Willd. (Rubiaceae): A Review on Phytochemistry, Pharmacology, Quality Control and Pharmacokinetics. Molecules, 21(6), 710. https://doi.org/10.3390/molecules21060710
Chen, Y., Miao, Y., Huang, L., Li, J., Sun, H., Zhao, Y., Yang, J., & Zhou, W. (2014). Antioxidant activities of saponins extracted from Radix Trichosanthis: an in vivo and in vitro evaluation. BMC Complementary and Alternative Medicine, 14, 86. https://doi.org/10.1186/1472-6882-14-86
Do, T. T. H., Tang, M. K., Nguyen, T. K. H., Nguyen, T. E., Yasuaki, T., & Nguyen, T. P. (2020). Anh huong cua do man len chi tieu sinh ly, tang truong va hoat tinh men tieu hoa cua ca loc (Channa striata) giai doan ca bot len ca huong. Can Tho University Journal of Science, 56, 11-19. https://doi.org/10.22144/ctu.jsi.2020.002
Everds, N. (2004). Hematology of the Mouse. In The Laboratory Mouse (pp. 271-286). https://doi.org/10.1016/B978-012336425-8/50070-4
Heim, E. K., Tagliaferro, A. R., & Bobilya, D. J. (2002). Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry, 13(10), 572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
Ho, H. T. D. (2005). Nghiên cứu hoạt tính sinh học của cây thuốc Việt Nam với một số quy trình thử nghiệm mới [Investigation of biological activities of medicinal plants in Viet Nam using new assays].Government level topic, Natural Sciences Program.
Huang, Q., Zhang, S., Zheng, L., He, M., Huang, R., & Lin, X. (2012). Hepatoprotective effects of total saponins isolated from Taraphochlamys affinis against carbon tetrachloride induced liver injury in rats. Food and Chemical Toxicology, 50(3-4), 713-718. https://doi.org/10.1016/j.fct.2011.12.009
Huo, H. Z., Wang, B., Liang, Y. K., Bao, Y. Y., & Gu, Y. (2011). Hepatoprotective and antioxidant effects of licorice extract against CCl4-induced oxidative damage in rats. International Journal of Molecular Sciences, 12(10), 6529-6543. https://doi.org/10.3390/ijms12106529
Huynh, K. Y., Nguyen, T. T., Tran, T. M., Phung, T. H., Nguyen, G. S., Tran, H. L., Truong, T. T. T., & Le, T. T. (2021). Hoat tinh khang oxy hoa va khang khuan cua cao chiet ethanol tu la coc trang (Lumnitzera racemosa Willd) [Antioxidant and antimicrobial activities of ethanolic extract of Lumnitzera racemosa Willd. leaves]. Can Tho University Journal of Science, 57(5A), 44-51. https://doi.org/10.22144/ctu.jvn.2021.140
Ikwuka, D. C., Anyaehie, B. U., Iyare, E. E., Ugwu, P., Katchy, A. U., Igbokwe, G. E., Uzoigwe, J. U., Ezeokafor, E. N., & Ezeudensi, K. L. (2021). Assessment of Hematological and Serum Electrolytes Effects of Intermittent Fasting on Mice. Journal of Pharmaceutical Research International, 33(62B), 207-216. https://doi.org/10.9734/JPRI/2021/v33i62B35188
Ly, T. N., Shimoyamada, M., & Yamauchi, R. (2006). Isolation and characterization of rosmarinic acid oligomers in Celastrus hindsii Benth leaves and their antioxidative activity. Journal of Agricultural and Food Chemistry, 54(11), 3786-3793. https://doi.org/10.1021/jf052743f
Macáková, K., Afonso, R., Saso, L., & Mladěnka, P. (2019). The influence of alkaloids on oxidative stress and related signaling pathways. Free Radical Biology and Medicine, 134, 429-444. https://doi.org/10.1016/j.freeradbiomed.2019.01.026
Maisetta, G., Batoni, G., Caboni, P., Esin, S., Rinaldi, A. C., & Zucca, P. (2019). Tannin profile, antioxidant properties, and antimicrobial activity of extracts from two Mediterranean species of parasitic plant Cytinus. BMC Complementary and Alternative Medicine, 19(1), Article 82. https://doi.org/10.1186/s12906-019-2487-7
Martínez-Calva, I., Campos-Apáez, A., Rosales-Vega, E., & Mourelle, M. (1984). Vitamin E improves membrane lipid alterations induced by CCl4 intoxication. Journal of Applied Toxicology, 4(5), 270-272. https://doi.org/10.1002/jat.2550040513
Nemzek, J. A., Bolgos, G. L., Williams, B. A., & Remick, D. G. (2001). Differences in normal values for murine white blood cell counts and other hematological parameters based on sampling site. Inflammation research, 50(10), 523-527. https://doi.org/10.1007/PL00000229
Nguyen, H. M., Ha, Q. T., Kim, S. T., Ha, T. H. L., Duong, H. T. Q., & Nguyen, T. T. H. (2023). Tac dung bao ve gan cua vien nang bach hoa xa thiet thao-ban chi lien tren chuot bi ton thuong gan boi paracetamol va ethanol. Hong Bang International University Journal of Science, 24, 171-180. https://doi.org/10.59294/HIUJS.24.2023.327
Nguyen, T. T. H., & Vo, V. T. (2019). Laboratory practice human and animal physiology [Thuc hanh sinh li hoc nguoi va dong vat]. Ho Chi Minh City University of Education Publisher.
O'Connell, K. E., Mikkola, A. M., Stepanek, A. M., Vernet, A., Hall, C. D., Sun, C. C., Yildirim, E., Staropoli, J. F., Lee, J. T., & Brown, D. E. (2015). Practical murine hematopathology: A comparative review and implications for research. Comparative medicine, 65(2), 96-113. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408895/#:~:text=Mice%20in%20health%20have%20many,marrow%20lymphocyte%20percentages%2C%20variable%20leukocyte
Paarvanova, B., Tacheva, B., Savova, G., Karabaliev, M., & Georgieva, R. (2023). Hemolysis by Saponin Is Accelerated at Hypertonic Conditions. Molecules, 28(20), 7096. https://doi.org/10.3390/molecules28207096
Pannu, A., Goyal, R. K. (2021). Pharmacognostical characterization of whole scutellaria barbata herbfor quality control assessment. Innovat International Journal of Medical & Pharmaceutical Sciences, 6(4), 1-5. Retrieved from https://innovatpublisher.com/index.php/iijmps/article/view/152/339
Permana, D., Lajis, N., Hj., Abas, F., Othman, A. G., Ahmad, R., Kitajima, M., Takayama, H., & Aimi, N. (2003). Antioxidative Constituents of Hedyotis diffusa Willd.. Natural Product Sciences, 9(1), 7-9. https://koreascience.kr/article/JAKO200303041140504.pdf
Pham, D. C., Nguyen, H. C., Nguyen, T. L., Ho, H. L., Trinh, T. K., Riyaphan, J., & Weng, C. F. (2020). Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities. Biomed Research International, 2020, Article 3497107. https://doi.org/10.1155/2020/3497107
Phan, K. D., Vo, T. M. H., Trinh, D. H. M., Le, T. D., Chan, C. L., Nguyen, T. T., & Dai, T. X. T. (2020). Nghien cuu mot so hoat tinh sinh hoc cua cao chiet methanol cay luoi ran trang (Hedyotis diffusa willd.). Can Tho University Journal of Science, 56, 103-114. https://doi.org/10.22144/ctu.jsi.2020.118
Sarin, S. K., Kumar, M., Eslam, M., George, J., Al Mahtab, M., Akbar, S. M. F., Jia, J., Tian, Q., Aggarwal, R., Muljono, D. H., Omata, M., Ooka, Y., Han, K. H., Lee, H. W., Jafri, W., Butt, A. S., Chong, C. H., Lim, S. G., Pwu, R. F., & Chen, D. S. (2020). Liver diseases in the Asia-Pacific region: a Lancet Gastroenterology & Hepatology Commission. The lancet Gastroenterology & hepatology, 5(2), 167-228. https://doi.org/10.1016/S2468-1253(19), 30342-5
Scholten, D., Trebicka, J., Liedtke, C., & Weiskirchen, R. (2015). The carbon tetrachloride model in mice. Laboratory Animals, 49(51), 4-11. https://doi.org/10.1177/0023677215571192
Tran, D. V., Chan, D. X., & La, H. A. (2021). α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials. Molecules, 26(23), Article 7248. https://doi.org/10.3390/molecules26237248
Tran, T. L. G., Nguyen, T. N., Nguyen, K. D., Le, N. T. L., Trinh, T. B., Nguyen, P. A. U, & Bui, D. T. (2017). Antioxidant properties of flavonoids extracted from some vietnamese medicinal plants. European Journal of Advanced Research in Biological and Life Sciences, 5(1), 1-8. https://www.idpublications.org/wp-content/uploads/2016/12/Full-Paper-antioxidant-properties-of-flavonoids-extracted-from-some-vietnamese.pdf
Tran, T. Y. N. (2022). Hoat tinh khang oxy hoa cua cao chiet cay Hy thiem (Siegesbeckia orientalis L.) [Evaluation of the antioxidant activity of methanol extracts of Siegesbeckia orientalis L.]. Thu Dau Mot University Journal of Science, 3(58), 69-78. https://doi.org/10.37550/tdmu.VJS/2022.03.308
World Health Organization. (2019). Viet Nam. https://data.who.int/countries/704
Yan, C., Kong, F., & Ou, X. (2012). Antioxidant and anti-glycated activities of polysaccharides in vitro isolated from Hedyotis diffusa Willd. Journal of Medicinal Plants Research, 6(14), 2895-2900. https://doi.org/10.5897/JMPR11.1769