DETERMINATION OF GENETIC VARIATIONS IN STRAWBERRIES INDUCED BY X-RAY IRRADIATION
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Abstract
This study used three high-resolution DNA fingerprinting techniques to evaluate genetic variations in strawberry samples: Random Amplified Polymorphic DNA (RAPD), which enables completely random amplification; Semi-random Start Codon Targeted (SCoT) markers, which focus on gene start codons; and CAAT Box-Derived Polymorphism (CBDP), which targets the CAAT-box sequence in gene promoters. These techniques offer high sensitivity, significant polymorphism, cost-effectiveness, and ease of implementation, making them ideal for reliable sample discrimination and the assessment of genetic stability. A low-energy X-ray irradiation system at Dalat University was employed to assess the genetic variability of strawberry samples collected from Lam Dong Province. To assess genome stability, the samples were irradiated under two experimental conditions: (a) varying radiation doses from 0 to 1,200 Gy at a fixed dose rate of 13.870 Gy/min, and (b) a fixed dose of 1,000 Gy with varying dose rates ranging from 3.370 to 24.450 Gy/min. The analysis results consistently showed negligible genetic variation across all irradiated samples. This finding is crucial because it supports the quality assurance of the irradiated strawberry products and indicates that X-ray irradiation does not cause adverse genetic effects that may affect consumer safety.
Keywords
dose, irradiation, strawberry, X-ray
Article Details
References
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