XÁC ĐỊNH BIẾN ĐỘNG DI TRUYỀN CỦA DÂU TÂY KHI CHIẾU XẠ BẰNG TIA X
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Abstract
ABSTRACT
In this study, the author used three high-resolution DNA fingerprinting techniques were employed to evaluate genetic variations in strawberry samples: Random Amplified Polymorphism DNA (RAPD) for completely random amplification; the semi-random Start Codon Targeted (SCoT), focusing on gene Start Codons; and CAAT Box-Derived Polymorphism (CBDP), which targets the CAAT box sequence specific to the gene promoter. These methods offer high sensitivity, significant polymorphism, cost-effectiveness, and ease of execution, making them ideal for reliable sample discrimination and the determination of genetic stability.
The low-energy X-ray irradiation system at Dalat University was employed to assess the genetic variability of strawberries sourced from Lam Dong province. To assess the stability of the strawberry genome, samples were exposed to X-ray irradiation under two conditions: a variable dose (0 to 1,200 Gy) at a fixed dose rate of 13.870 Gy/min, and a fixed dose of 1,000 Gy with variable dose rates (ranging from 3.370 to 24.450 Gy/min). The analysis consistently showed that the observed genetic variability in all irradiated samples was negligible. This finding is crucial, as it validates the quality assurance of the irradiated product and confirms no adverse genetic impact on consumers.
Keywords: Irradiation; Strawberry; Dose; X-ray
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References
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