EFFECTS OF THE DURATION OF ARTIFICIAL UVA IRRADIATION EXPOSURE ON SKIN DAMAGE LEVELS IN ALBINO MICE
Main Article Content
Abstract
The rise in the UV ray index, particularly UVA, has played a role in the heightened pace of skin aging. The rate at which skin ages fluctuates depending on the duration of exposure to UV rays. The objective of this study was to examine the impact of varying durations of artificial UVA irradiation on the level of skin damage observed in albino mice. It was observed that as the duration of irradiation increased gradually (3 hours, 6 hours, and 9 hours of exposure), the damage to the mice's skin also increased progressively with the irradiation dose remained constant (24 mJ/cm2/minute): elevated external aging signs, augmented epidermal thickness, and intensified breakdown of collagen. Furthermore, this study offers predictions on the aging patterns of mouse skin when subjected to artificial UVA irradiation timelines, facilitating other experiments to arrange high efficiency. It is necessary to research the effect of artificial UVA irradiation level on rat skin structure to have more evidence to evaluate the effect of UVA on rat skin structure.
Keywords
Mus musculus var. albino, skin aging, skin structure of mice, UVA, UV rays
Article Details
References
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Komatsu, T., Sasaki, S., Manabe, Y., Hirata, T., & Sugawara, T. (2017). Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice. PLoS ONE, 12(2), e0171178. doi:10.1371/journal.pone.0171178
Kovacs, D., Raffa, S., Flori, E., Aspite, N., Briganti, S., Cardinali, G., . . . Picardo, M. (2009). Keratinocyte growth factor down-regulates intracellular ROS production induced by UVB. J Dermatol Sci, 54(2), 106-113. doi:10.1016/j.jdermsci.2009.01.005
Lee, J. S., Park, K. Y., Min, H. G., Lee, S. J., Kim, J. J., Choi, J. S., . . . Cha, H. J. (2010). Negative regulation of stress-induced matrix metalloproteinase-9 by Sirt1 in skin tissue. Exp Dermatol, 19(12), 1060-1066. doi:10.1111/j.1600-0625.2010.01129.x
Nagase, H., Visse, R., & Murphy, G. (2006). Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res, 69(3), 562-573. doi:10.1016/j.cardiores.2005.12.002
Nguyen, T. T. H., Bui, T. K. N., Truong, V. T., & Pham, V. N. (2019). Anh huong cua thoi gian chieu UVA len khoi luong co the, so luong te bao mau va noi quan cua chuot nhat trang (Mus musculus var. albino) [Effects of UVA light exposure on the body weight, the blood cells and internal organs of albino mouse (Mus musculus var. albino)]. Ho Chi Minh City University of Education Journal of Science, 16(12), 1034-1652.
Philips, N., Burchill, D., O'Donoghue, D., Keller, T., & Gonzalez, S. (2004). Identification of benzene metabolites in dermal fibroblasts as nonphenolic: regulation of cell viability, apoptosis, lipid peroxidation and expression of matrix metalloproteinase 1 and elastin by benzene metabolites. Skin Pharmacol Physiol, 17(3), 147-152. doi:10.1159/000077242
Philips, N., Chalensouk-Khaosaat, J., & Gonzalez, S. (2018). Simulation of the elastin and fibrillin in non-irradiated or UVA radiated fibroblasts, and direct inhibition of elastase or matrix metalloptoteinases activity by nicotinamide or its derivatives. J Cosmet Sci, 69(1), 47-56.
Philips, N., Hwang, H., Chauhan, S., Leonardi, D., & Gonzalez, S. (2010). Stimulation of cell proliferation and expression of matrixmetalloproteinase-1 and interluekin-8 genes in dermal fibroblasts by copper. Connect Tissue Res, 51(3), 224-229. doi:10.3109/03008200903288431
Philips, N., Tuason, M., Chang, T., Lin, Y., Tahir, M., & Rodriguez, S. G. (2009). Differential effects of ceramide on cell viability and extracellular matrix remodeling in keratinocytes and fibroblasts. Skin Pharmacol Physiol, 22(3), 151-157. doi:10.1159/000208168
Pillai, S., Oresajo, C., & Hayward, J. (2005). Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review. Int J Cosmet Sci, 27(1), 17-34. doi:10.1111/j.1467-2494.2004.00241.x
Quan, T., He, T., Kang, S., Voorhees, J. J., & Fisher, G. J. (2004). Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am J Pathol, 165(3), 741-751.
Slominski, A. T., Zmijewski, M. A., Skobowiat, C., Zbytek, B., Slominski, R. M., & Steketee, J. D. (2012). Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system. Adv Anat Embryol Cell Biol, 212, v, vii, 1-115.
University of Virginia Library - Research Data Services + Sciences. Fitting and Interpreting a Proportional Odds Model. Retrieved from https://data.library.virginia.edu/fitting-and-interpreting-a-proportional-odds-model/
Wang, L., Zhang, X., Li, Y. X., Xu, L. Q., Li, C. L., Zhang, Z. B., . . . Li, Y. C. (2016). Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin. Evid Based Complement Alternat Med, 2016, 9623957. doi:10.1155/2016/9623957
Bhattacharyya, T. K., Hsia, Y., Weeks, D. M., Dixon, T. K., Lepe, J., & Thomas, J. R. (2017). Association of Diet With Skin Histological Features in UV-B-Exposed Mice. JAMA Facial Plast Surg, 19(5), 399-405. doi:10.1001/jamafacial.2017.0060
Chung, J. H., & Eun, H. C. (2007). Angiogenesis in skin aging and photoaging. J Dermatol, 34(9), 593-600. doi:10.1111/j.1346-8138.2007.00341.x
Coelho, S. G., Choi, W., Brenner, M., Miyamura, Y., Yamaguchi, Y., Wolber, R., . . . Hearing, V. J. (2009). Short- and long-term effects of UV radiation on the pigmentation of human skin.
J Investig Dermatol Symp Proc, 14(1), 32-35. doi:10.1038/jidsymp.2009.10
D'Orazio, J., Jarrett, S., Amaro-Ortiz, A., & Scott, T. (2013). UV radiation and the skin. Int J Mol Sci, 14(6), 12222-12248. doi:10.3390/ijms140612222
De Gruijl, F. R. (2000). Photocarcinogenesis: UVA vs UVB. In Methods Enzymol (Vol. 319, pp. 359-366): Elsevier.
Dong, K. K., Damaghi, N., Picart, S. D., Markova, N. G., Obayashi, K., Okano, Y., . . .
Yarosh, D. B. (2008). UV-induced DNA damage initiates release of MMP-1 in human skin. Exp Dermatol, 17(12), 1037-1044. doi:10.1111/j.1600-0625.2008.00747.x
Feng, X. X., Yu, X. T., Li, W. J., Kong, S. Z., Liu, Y. H., Zhang, X., . . . Lin, Z. X. (2014). Effects of topical application of patchouli alcohol on the UV-induced skin photoaging in mice. Eur J Pharm Sci, 63, 113-123. doi:10.1016/j.ejps.2014.07.001
Fischer, A. H., Jacobson, K. A., Rose, J., & Zeller, R. (2008). Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc, 2008, pdb prot4986. doi:10.1101/pdb.prot4986
Fisher, G. J., Choi, H. C., Bata-Csorgo, Z., Shao, Y., Datta, S., Wang, Z. Q., . . . Voorhees, J. J. (2001). Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo. J Invest Dermatol, 117(2), 219-226. doi:10.1046/j.0022-202x.2001.01432.x
Fisher, G. J., Kang, S., Varani, J., Bata-Csorgo, Z., Wan, Y., Datta, S., & Voorhees, J. J. (2002). Mechanisms of photoaging and chronological skin aging. Arch Dermatol, 138(11), 1462-1470. doi:10.1001/archderm.138.11.1462
Fisher, G. J., Wang, Z. Q., Datta, S. C., Varani, J., Kang, S., & Voorhees, J. J. (1997). Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med, 337(20), 1419-1428. doi:10.1056/NEJM199711133372003
Fuchs, E. (2007). Scratching the surface of skin development. Nature, 445(7130), 834-842. doi:10.1038/nature05659
Hwang, K. A., Yi, B. R., & Choi, K. C. (2011). Molecular mechanisms and in vivo mouse models of skin aging associated with dermal matrix alterations. Lab Anim Res, 27(1), 1-8. doi:10.5625/lar.2011.27.1.1
Kim, S. R., Jung, Y. R., An, H. J., Kim, D. H., Jang, E. J., Choi, Y. J., . . . Chung, H. Y. (2013). Anti-wrinkle and anti-inflammatory effects of active garlic components and the inhibition of MMPs via NF-kappaB signaling. PLoS ONE, 8(9), e73877. doi:10.1371/journal.pone.0073877
Kim, Y. J., Kim, H. N., Shin, M. S., & Choi, B. T. (2015). Thread Embedding Acupuncture Inhibits Ultraviolet B Irradiation-Induced Skin Photoaging in Hairless Mice. Evid Based Complement Alternat Med, 2015, 539172. doi:10.1155/2015/539172
Komatsu, T., Sasaki, S., Manabe, Y., Hirata, T., & Sugawara, T. (2017). Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice. PLoS ONE, 12(2), e0171178. doi:10.1371/journal.pone.0171178
Kovacs, D., Raffa, S., Flori, E., Aspite, N., Briganti, S., Cardinali, G., . . . Picardo, M. (2009). Keratinocyte growth factor down-regulates intracellular ROS production induced by UVB. J Dermatol Sci, 54(2), 106-113. doi:10.1016/j.jdermsci.2009.01.005
Lee, J. S., Park, K. Y., Min, H. G., Lee, S. J., Kim, J. J., Choi, J. S., . . . Cha, H. J. (2010). Negative regulation of stress-induced matrix metalloproteinase-9 by Sirt1 in skin tissue. Exp Dermatol, 19(12), 1060-1066. doi:10.1111/j.1600-0625.2010.01129.x
Nagase, H., Visse, R., & Murphy, G. (2006). Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res, 69(3), 562-573. doi:10.1016/j.cardiores.2005.12.002
Nguyen, T. T. H., Bui, T. K. N., Truong, V. T., & Pham, V. N. (2019). Anh huong cua thoi gian chieu UVA len khoi luong co the, so luong te bao mau va noi quan cua chuot nhat trang (Mus musculus var. albino) [Effects of UVA light exposure on the body weight, the blood cells and internal organs of albino mouse (Mus musculus var. albino)]. Ho Chi Minh City University of Education Journal of Science, 16(12), 1034-1652.
Philips, N., Burchill, D., O'Donoghue, D., Keller, T., & Gonzalez, S. (2004). Identification of benzene metabolites in dermal fibroblasts as nonphenolic: regulation of cell viability, apoptosis, lipid peroxidation and expression of matrix metalloproteinase 1 and elastin by benzene metabolites. Skin Pharmacol Physiol, 17(3), 147-152. doi:10.1159/000077242
Philips, N., Chalensouk-Khaosaat, J., & Gonzalez, S. (2018). Simulation of the elastin and fibrillin in non-irradiated or UVA radiated fibroblasts, and direct inhibition of elastase or matrix metalloptoteinases activity by nicotinamide or its derivatives. J Cosmet Sci, 69(1), 47-56.
Philips, N., Hwang, H., Chauhan, S., Leonardi, D., & Gonzalez, S. (2010). Stimulation of cell proliferation and expression of matrixmetalloproteinase-1 and interluekin-8 genes in dermal fibroblasts by copper. Connect Tissue Res, 51(3), 224-229. doi:10.3109/03008200903288431
Philips, N., Tuason, M., Chang, T., Lin, Y., Tahir, M., & Rodriguez, S. G. (2009). Differential effects of ceramide on cell viability and extracellular matrix remodeling in keratinocytes and fibroblasts. Skin Pharmacol Physiol, 22(3), 151-157. doi:10.1159/000208168
Pillai, S., Oresajo, C., & Hayward, J. (2005). Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review. Int J Cosmet Sci, 27(1), 17-34. doi:10.1111/j.1467-2494.2004.00241.x
Quan, T., He, T., Kang, S., Voorhees, J. J., & Fisher, G. J. (2004). Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am J Pathol, 165(3), 741-751.
Slominski, A. T., Zmijewski, M. A., Skobowiat, C., Zbytek, B., Slominski, R. M., & Steketee, J. D. (2012). Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system. Adv Anat Embryol Cell Biol, 212, v, vii, 1-115.
University of Virginia Library - Research Data Services + Sciences. Fitting and Interpreting a Proportional Odds Model. Retrieved from https://data.library.virginia.edu/fitting-and-interpreting-a-proportional-odds-model/
Wang, L., Zhang, X., Li, Y. X., Xu, L. Q., Li, C. L., Zhang, Z. B., . . . Li, Y. C. (2016). Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin. Evid Based Complement Alternat Med, 2016, 9623957. doi:10.1155/2016/9623957