ẢNH HƯỞNG CỦA THỜI GIAN CHIẾU TIA UVA NHÂN TẠO LÊN MỨC ĐỘ TỔN THƯƠNG DA CHUỘT NHẮT TRẮNG (Mus musculus var. albino)
Nội dung chính của bài viết
Tóm tắt
Sự gia tăng chỉ số của tia UV (Ultraviolet), đặc biệt UVA, là một yếu tố góp phần làm gia tăng tốc độ lão hoá da. Tốc độ lão hoá da thay đổi tùy thuộc vào thời gian tiếp xúc với tia UV. Nghiên cứu này được thực hiện nhằm đánh giá được ảnh hưởng của thời gian chiếu tia UVA nhân tạo lên mức độ tổn thương da chuột nhắt trắng. Kết quả cho thấy, cùng một liều chiếu (24 mJ/cm2/phút), sự tổn thương lên da chuột có xu hướng tăng dần theo sự tăng dần thời gian chiếu (3 giờ, 6 giờ, và 9 giờ chiếu): tăng mức độ lão hoá bên ngoài, tăng độ dày biểu bì và làm tăng sự đứt gãy cấu trúc collagen. Nghiên cứu này còn dự đoán được các mức độ lão hoá da chuột với các mốc thời gian chiếu UVA nhân tạo giúp cho các đợt thí nghiệm khác bố trí đạt hiệu quả cao. Cần thực hiện nghiên cứu ảnh hưởng của liều chiếu UVA nhân tạo lên cấu trúc da chuột để có thêm căn cứ về đánh giá ảnh hưởng của UVA lên cấu trúc da chuột.
Từ khóa
cấu trúc da chuột, chuột nhắt trắng, lão hoá da, UVA, tia UV
Chi tiết bài viết
Tài liệu tham khảo
Amaro-Ortiz, A., Yan, B., & D'Orazio, J. A. (2014). Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules, 19(5), 6202-6219. doi:10.3390/molecules19056202
Bhattacharyya, T. K., Hsia, Y., Weeks, D. M., Dixon, T. K., Lepe, J., & Thomas, J. R. (2017). Association of Diet With Skin Histological Features in UV-B-Exposed Mice. JAMA Facial Plast Surg, 19(5), 399-405. doi:10.1001/jamafacial.2017.0060
Chung, J. H., & Eun, H. C. (2007). Angiogenesis in skin aging and photoaging. J Dermatol, 34(9), 593-600. doi:10.1111/j.1346-8138.2007.00341.x
Coelho, S. G., Choi, W., Brenner, M., Miyamura, Y., Yamaguchi, Y., Wolber, R., . . . Hearing, V. J. (2009). Short- and long-term effects of UV radiation on the pigmentation of human skin.
J Investig Dermatol Symp Proc, 14(1), 32-35. doi:10.1038/jidsymp.2009.10
D'Orazio, J., Jarrett, S., Amaro-Ortiz, A., & Scott, T. (2013). UV radiation and the skin. Int J Mol Sci, 14(6), 12222-12248. doi:10.3390/ijms140612222
De Gruijl, F. R. (2000). Photocarcinogenesis: UVA vs UVB. In Methods Enzymol (Vol. 319, pp. 359-366): Elsevier.
Dong, K. K., Damaghi, N., Picart, S. D., Markova, N. G., Obayashi, K., Okano, Y., . . .
Yarosh, D. B. (2008). UV-induced DNA damage initiates release of MMP-1 in human skin. Exp Dermatol, 17(12), 1037-1044. doi:10.1111/j.1600-0625.2008.00747.x
Feng, X. X., Yu, X. T., Li, W. J., Kong, S. Z., Liu, Y. H., Zhang, X., . . . Lin, Z. X. (2014). Effects of topical application of patchouli alcohol on the UV-induced skin photoaging in mice. Eur J Pharm Sci, 63, 113-123. doi:10.1016/j.ejps.2014.07.001
Fischer, A. H., Jacobson, K. A., Rose, J., & Zeller, R. (2008). Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc, 2008, pdb prot4986. doi:10.1101/pdb.prot4986
Fisher, G. J., Choi, H. C., Bata-Csorgo, Z., Shao, Y., Datta, S., Wang, Z. Q., . . . Voorhees, J. J. (2001). Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo. J Invest Dermatol, 117(2), 219-226. doi:10.1046/j.0022-202x.2001.01432.x
Fisher, G. J., Kang, S., Varani, J., Bata-Csorgo, Z., Wan, Y., Datta, S., & Voorhees, J. J. (2002). Mechanisms of photoaging and chronological skin aging. Arch Dermatol, 138(11), 1462-1470. doi:10.1001/archderm.138.11.1462
Fisher, G. J., Wang, Z. Q., Datta, S. C., Varani, J., Kang, S., & Voorhees, J. J. (1997). Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med, 337(20), 1419-1428. doi:10.1056/NEJM199711133372003
Fuchs, E. (2007). Scratching the surface of skin development. Nature, 445(7130), 834-842. doi:10.1038/nature05659
Hwang, K. A., Yi, B. R., & Choi, K. C. (2011). Molecular mechanisms and in vivo mouse models of skin aging associated with dermal matrix alterations. Lab Anim Res, 27(1), 1-8. doi:10.5625/lar.2011.27.1.1
Kim, S. R., Jung, Y. R., An, H. J., Kim, D. H., Jang, E. J., Choi, Y. J., . . . Chung, H. Y. (2013). Anti-wrinkle and anti-inflammatory effects of active garlic components and the inhibition of MMPs via NF-kappaB signaling. PLoS ONE, 8(9), e73877. doi:10.1371/journal.pone.0073877
Kim, Y. J., Kim, H. N., Shin, M. S., & Choi, B. T. (2015). Thread Embedding Acupuncture Inhibits Ultraviolet B Irradiation-Induced Skin Photoaging in Hairless Mice. Evid Based Complement Alternat Med, 2015, 539172. doi:10.1155/2015/539172
Komatsu, T., Sasaki, S., Manabe, Y., Hirata, T., & Sugawara, T. (2017). Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice. PLoS ONE, 12(2), e0171178. doi:10.1371/journal.pone.0171178
Kovacs, D., Raffa, S., Flori, E., Aspite, N., Briganti, S., Cardinali, G., . . . Picardo, M. (2009). Keratinocyte growth factor down-regulates intracellular ROS production induced by UVB. J Dermatol Sci, 54(2), 106-113. doi:10.1016/j.jdermsci.2009.01.005
Lee, J. S., Park, K. Y., Min, H. G., Lee, S. J., Kim, J. J., Choi, J. S., . . . Cha, H. J. (2010). Negative regulation of stress-induced matrix metalloproteinase-9 by Sirt1 in skin tissue. Exp Dermatol, 19(12), 1060-1066. doi:10.1111/j.1600-0625.2010.01129.x
Nagase, H., Visse, R., & Murphy, G. (2006). Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res, 69(3), 562-573. doi:10.1016/j.cardiores.2005.12.002
Nguyen, T. T. H., Bui, T. K. N., Truong, V. T., & Pham, V. N. (2019). Anh huong cua thoi gian chieu UVA len khoi luong co the, so luong te bao mau va noi quan cua chuot nhat trang (Mus musculus var. albino) [Effects of UVA light exposure on the body weight, the blood cells and internal organs of albino mouse (Mus musculus var. albino)]. Ho Chi Minh City University of Education Journal of Science, 16(12), 1034-1652.
Philips, N., Burchill, D., O'Donoghue, D., Keller, T., & Gonzalez, S. (2004). Identification of benzene metabolites in dermal fibroblasts as nonphenolic: regulation of cell viability, apoptosis, lipid peroxidation and expression of matrix metalloproteinase 1 and elastin by benzene metabolites. Skin Pharmacol Physiol, 17(3), 147-152. doi:10.1159/000077242
Philips, N., Chalensouk-Khaosaat, J., & Gonzalez, S. (2018). Simulation of the elastin and fibrillin in non-irradiated or UVA radiated fibroblasts, and direct inhibition of elastase or matrix metalloptoteinases activity by nicotinamide or its derivatives. J Cosmet Sci, 69(1), 47-56.
Philips, N., Hwang, H., Chauhan, S., Leonardi, D., & Gonzalez, S. (2010). Stimulation of cell proliferation and expression of matrixmetalloproteinase-1 and interluekin-8 genes in dermal fibroblasts by copper. Connect Tissue Res, 51(3), 224-229. doi:10.3109/03008200903288431
Philips, N., Tuason, M., Chang, T., Lin, Y., Tahir, M., & Rodriguez, S. G. (2009). Differential effects of ceramide on cell viability and extracellular matrix remodeling in keratinocytes and fibroblasts. Skin Pharmacol Physiol, 22(3), 151-157. doi:10.1159/000208168
Pillai, S., Oresajo, C., & Hayward, J. (2005). Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review. Int J Cosmet Sci, 27(1), 17-34. doi:10.1111/j.1467-2494.2004.00241.x
Quan, T., He, T., Kang, S., Voorhees, J. J., & Fisher, G. J. (2004). Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am J Pathol, 165(3), 741-751.
Slominski, A. T., Zmijewski, M. A., Skobowiat, C., Zbytek, B., Slominski, R. M., & Steketee, J. D. (2012). Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system. Adv Anat Embryol Cell Biol, 212, v, vii, 1-115.
University of Virginia Library - Research Data Services + Sciences. Fitting and Interpreting a Proportional Odds Model. Retrieved from https://data.library.virginia.edu/fitting-and-interpreting-a-proportional-odds-model/
Wang, L., Zhang, X., Li, Y. X., Xu, L. Q., Li, C. L., Zhang, Z. B., . . . Li, Y. C. (2016). Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin. Evid Based Complement Alternat Med, 2016, 9623957. doi:10.1155/2016/9623957
Bhattacharyya, T. K., Hsia, Y., Weeks, D. M., Dixon, T. K., Lepe, J., & Thomas, J. R. (2017). Association of Diet With Skin Histological Features in UV-B-Exposed Mice. JAMA Facial Plast Surg, 19(5), 399-405. doi:10.1001/jamafacial.2017.0060
Chung, J. H., & Eun, H. C. (2007). Angiogenesis in skin aging and photoaging. J Dermatol, 34(9), 593-600. doi:10.1111/j.1346-8138.2007.00341.x
Coelho, S. G., Choi, W., Brenner, M., Miyamura, Y., Yamaguchi, Y., Wolber, R., . . . Hearing, V. J. (2009). Short- and long-term effects of UV radiation on the pigmentation of human skin.
J Investig Dermatol Symp Proc, 14(1), 32-35. doi:10.1038/jidsymp.2009.10
D'Orazio, J., Jarrett, S., Amaro-Ortiz, A., & Scott, T. (2013). UV radiation and the skin. Int J Mol Sci, 14(6), 12222-12248. doi:10.3390/ijms140612222
De Gruijl, F. R. (2000). Photocarcinogenesis: UVA vs UVB. In Methods Enzymol (Vol. 319, pp. 359-366): Elsevier.
Dong, K. K., Damaghi, N., Picart, S. D., Markova, N. G., Obayashi, K., Okano, Y., . . .
Yarosh, D. B. (2008). UV-induced DNA damage initiates release of MMP-1 in human skin. Exp Dermatol, 17(12), 1037-1044. doi:10.1111/j.1600-0625.2008.00747.x
Feng, X. X., Yu, X. T., Li, W. J., Kong, S. Z., Liu, Y. H., Zhang, X., . . . Lin, Z. X. (2014). Effects of topical application of patchouli alcohol on the UV-induced skin photoaging in mice. Eur J Pharm Sci, 63, 113-123. doi:10.1016/j.ejps.2014.07.001
Fischer, A. H., Jacobson, K. A., Rose, J., & Zeller, R. (2008). Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc, 2008, pdb prot4986. doi:10.1101/pdb.prot4986
Fisher, G. J., Choi, H. C., Bata-Csorgo, Z., Shao, Y., Datta, S., Wang, Z. Q., . . . Voorhees, J. J. (2001). Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo. J Invest Dermatol, 117(2), 219-226. doi:10.1046/j.0022-202x.2001.01432.x
Fisher, G. J., Kang, S., Varani, J., Bata-Csorgo, Z., Wan, Y., Datta, S., & Voorhees, J. J. (2002). Mechanisms of photoaging and chronological skin aging. Arch Dermatol, 138(11), 1462-1470. doi:10.1001/archderm.138.11.1462
Fisher, G. J., Wang, Z. Q., Datta, S. C., Varani, J., Kang, S., & Voorhees, J. J. (1997). Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med, 337(20), 1419-1428. doi:10.1056/NEJM199711133372003
Fuchs, E. (2007). Scratching the surface of skin development. Nature, 445(7130), 834-842. doi:10.1038/nature05659
Hwang, K. A., Yi, B. R., & Choi, K. C. (2011). Molecular mechanisms and in vivo mouse models of skin aging associated with dermal matrix alterations. Lab Anim Res, 27(1), 1-8. doi:10.5625/lar.2011.27.1.1
Kim, S. R., Jung, Y. R., An, H. J., Kim, D. H., Jang, E. J., Choi, Y. J., . . . Chung, H. Y. (2013). Anti-wrinkle and anti-inflammatory effects of active garlic components and the inhibition of MMPs via NF-kappaB signaling. PLoS ONE, 8(9), e73877. doi:10.1371/journal.pone.0073877
Kim, Y. J., Kim, H. N., Shin, M. S., & Choi, B. T. (2015). Thread Embedding Acupuncture Inhibits Ultraviolet B Irradiation-Induced Skin Photoaging in Hairless Mice. Evid Based Complement Alternat Med, 2015, 539172. doi:10.1155/2015/539172
Komatsu, T., Sasaki, S., Manabe, Y., Hirata, T., & Sugawara, T. (2017). Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice. PLoS ONE, 12(2), e0171178. doi:10.1371/journal.pone.0171178
Kovacs, D., Raffa, S., Flori, E., Aspite, N., Briganti, S., Cardinali, G., . . . Picardo, M. (2009). Keratinocyte growth factor down-regulates intracellular ROS production induced by UVB. J Dermatol Sci, 54(2), 106-113. doi:10.1016/j.jdermsci.2009.01.005
Lee, J. S., Park, K. Y., Min, H. G., Lee, S. J., Kim, J. J., Choi, J. S., . . . Cha, H. J. (2010). Negative regulation of stress-induced matrix metalloproteinase-9 by Sirt1 in skin tissue. Exp Dermatol, 19(12), 1060-1066. doi:10.1111/j.1600-0625.2010.01129.x
Nagase, H., Visse, R., & Murphy, G. (2006). Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res, 69(3), 562-573. doi:10.1016/j.cardiores.2005.12.002
Nguyen, T. T. H., Bui, T. K. N., Truong, V. T., & Pham, V. N. (2019). Anh huong cua thoi gian chieu UVA len khoi luong co the, so luong te bao mau va noi quan cua chuot nhat trang (Mus musculus var. albino) [Effects of UVA light exposure on the body weight, the blood cells and internal organs of albino mouse (Mus musculus var. albino)]. Ho Chi Minh City University of Education Journal of Science, 16(12), 1034-1652.
Philips, N., Burchill, D., O'Donoghue, D., Keller, T., & Gonzalez, S. (2004). Identification of benzene metabolites in dermal fibroblasts as nonphenolic: regulation of cell viability, apoptosis, lipid peroxidation and expression of matrix metalloproteinase 1 and elastin by benzene metabolites. Skin Pharmacol Physiol, 17(3), 147-152. doi:10.1159/000077242
Philips, N., Chalensouk-Khaosaat, J., & Gonzalez, S. (2018). Simulation of the elastin and fibrillin in non-irradiated or UVA radiated fibroblasts, and direct inhibition of elastase or matrix metalloptoteinases activity by nicotinamide or its derivatives. J Cosmet Sci, 69(1), 47-56.
Philips, N., Hwang, H., Chauhan, S., Leonardi, D., & Gonzalez, S. (2010). Stimulation of cell proliferation and expression of matrixmetalloproteinase-1 and interluekin-8 genes in dermal fibroblasts by copper. Connect Tissue Res, 51(3), 224-229. doi:10.3109/03008200903288431
Philips, N., Tuason, M., Chang, T., Lin, Y., Tahir, M., & Rodriguez, S. G. (2009). Differential effects of ceramide on cell viability and extracellular matrix remodeling in keratinocytes and fibroblasts. Skin Pharmacol Physiol, 22(3), 151-157. doi:10.1159/000208168
Pillai, S., Oresajo, C., & Hayward, J. (2005). Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review. Int J Cosmet Sci, 27(1), 17-34. doi:10.1111/j.1467-2494.2004.00241.x
Quan, T., He, T., Kang, S., Voorhees, J. J., & Fisher, G. J. (2004). Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am J Pathol, 165(3), 741-751.
Slominski, A. T., Zmijewski, M. A., Skobowiat, C., Zbytek, B., Slominski, R. M., & Steketee, J. D. (2012). Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system. Adv Anat Embryol Cell Biol, 212, v, vii, 1-115.
University of Virginia Library - Research Data Services + Sciences. Fitting and Interpreting a Proportional Odds Model. Retrieved from https://data.library.virginia.edu/fitting-and-interpreting-a-proportional-odds-model/
Wang, L., Zhang, X., Li, Y. X., Xu, L. Q., Li, C. L., Zhang, Z. B., . . . Li, Y. C. (2016). Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin. Evid Based Complement Alternat Med, 2016, 9623957. doi:10.1155/2016/9623957