APPLICATION OF HEMATOLOGICAL REFERENCE SAMPLES FOR HEMATOLOGY EXTERNAL QUALITY ASSESSMENT (EQA) PROGRAMS IN PILOT SCALE
Main Article Content
Abstract
The Hematology External Quality Assessment (EQA) Program is one of the compulsory programs for laboratories in Vietnam. Implementing the EQA program requires materials that are reference material bio-samples. The research used the results of 60 participating laboratories to evaluate the results for three parameters: red blood cells (RBCs), white blood cells (WBCs), and platelets (PLTs). Laboratory results were evaluated using the coefficient of variation (CV%) and the z-score in 12 months by two groups of measurement methods (optical and impedance). The test results of the laboratories were analyzed by two groups: impedance measurement devices (Horiba ABX ABC Vet, Sysmex XP Series, Mindray BC 2000), optical measuring devices (Sysmex XN series, Horiba Yumizen H500, Mindray BC) 5100). Research results show that CV% on optical devices is lower than impedance measurement. The mean z-score was not acceptable mainly in white blood cell parameters. The percentage of laboratories failing to pass the z-score gradually decreased over 12 months. Laboratories participating in the hematology EQA Programs have improved significantly. The application of hematological reference samples provided by domestic units helps to be independent in technology and improve medical examination capacity.
Keywords
EQA, hematological reference samples, hematology testing, IQC, pseudo-leukocytes, pseudo-platelets
Article Details
References
Fritsma, G. A. (2013). Quality Assurance in Hematology and Hemostasis Testing. Hematology-E-Book: Clinical Principles and Applications (4th ed). Elsevier Health Sciences.
Kawai, Y., Nagai, Y., Ogawa, E., & Kondo, H. (2017). Japanese Society for Laboratory Hematology flow cytometric reference method of determining the differential leukocyte count: external quality assurance using fresh blood samples. International Journal of Laboratory Hematology, 39(2), 202-222. http:/doi.org/10.1111/ijlh.12607
Miller, W. G. (2003). Specimen materials, target values and commutability for external quality assessment (proficiency testing) schemes. Clinica chimica acta, 327(1-2), 25-37. http:/doi.org/ 10.1016/S0009-8981(02)00370-4
Sciacovelli, L., Secchiero, S., Zardo, L., Zaninotto, M., & Plebani, M. (2006). External quality assessment: an effective tool for clinical governance in laboratory medicine. Clinical Chemistry and Laboratory Medicine (CCLM), 44(6), 740-749. http:/doi.org/10.1515/CCLM.2006.133
Sciacovelli, L., Secchiero, S., Zardo, L., & Plebani, M. (2010). The role of the external quality assessment. Biochemia Medica, 20(2), 160-164. https://hrcak.srce.hr/53351
Turgeon, M. L. (2017). Clinical hematology: theory and procedures (6th ed). Lippincott Williams & Wilkins.
Vo, N. N., Tran, H. T., Truong, Q. T., & Nguyen, T. H. (2021). Optimization of Storage Medium for Hematological Reference Samples in External Quality Assessment. Applied Sciences, 11(18), Article 8777. http:/doi.org/10.3390/app11188777
Vo, N. N., Tran, H. T., Nguyen, T. H. P., Vu, D. D., Vo, T. S., & Nguyen, T. H. (2022). Determination of the Assigned Values of Blood Cells by an Impedance Method for Hematological Reference Samples Used in Hematology External Quality Assessment (EQA) Programs. Biomedicines, 10(12), Article 3169. http:/doi.org/10.3390/biomedicines10123169
Whitehead, T. P., & Woodford, F. P. (1981). External quality assessment of clinical laboratories in the United Kingdom. Journal of clinical pathology, 34(9), 947-957.
http:/doi.org/10.1136/jcp.34.9.947