TWO NEW STRAINS OF MICROALGAE Scenedesmus sp. RECENTLY ISOLATED AND IDENTIFIED BY 18S SEQUENCING FROM THE CAN GIO MANGROVE BIOSPHERE RESERVE
Main Article Content
Abstract
The microalgae Scenedesmus, belonging to the Chlorophyta phylum, is a widely distributed freshwater algae species. Many species from this genus are found in the Can Gio mangrove biosphere reserve, where they play a significant role in the aquatic environment. In this study, two microalgae strains, Scenedesmus CG01 and CG03, were newly isolated from two different sampling sites within the Can Gio mangrove biosphere reserve. These two strains exhibit similar morphological features typical of the genus Scenedesmus. PCR amplification, sequencing, and identification based on the 18S DNA sequence confirmed that both strains, CG01 and CG03, belong to Scenedesmus sp. The 18S DNA sequences of these strains differ from those of Scenedesmus sp. CCAP 217/7 and CCAP217/8 in GenBank. The phylogenetic analysis of the 18S sequences revealsthat CG01 and CG03 are in a distinct cluster, separate from the remaining samples. This research on the isolation and accurate identification of species provides an important foundation for future studies on the application of microalgae.
Keywords
18S, Can Gio, Scenedesmus
Article Details
References
Assunção, J., Pagels, F., & Guedes, A. C. (2023). Algae biorefinery: Strategies for a sustainable industry. In Algae materials: Applications benefitting health (pp. 399-433). Elsevier.
https://doi.org/10.1016/B978-0-443-18816-9.00020-4
Baudelet, P. H., Ricochon, G., Linder, M., & Muniglia, L. (2017). A new insight into cell walls of Chlorophyta. Algal Research, 25, 333-371. https://doi.org/10.1016/J.ALGAL.2017.04.008
Canter-Lund, H., & Lund, J. W. G. (1995). Freshwater algae: Their microscopic world explored. Biopress. https://doi.org/10.2216/i0031-8884-35-4-372b.1
Colla, E., Kalschne, D. L., da Silva-Buzanello, R. A., Canan, C., Drunkler, D. A., & Menegotto, A. L. L. (2020). Microalgae: A new and promising source of food. In Handbook of algal science, technology and medicine (pp. 507-518). Academic Press.
DOI: https://doi.org/10.1016/B978-0-12-818305-2.00032-2
Culture Collection of Algae and Protozoa (CCAP). (n.d.). Homepage. Retrieved May 1, 2024, from https://www.ccap.ac.uk/
Fawley, M. W., & Fawley, K. P. (2020). Identification of eukaryotic microalgal strains. Journal of Applied Phycology, 32(5), 2699-2709. https://doi.org/10.1007/s10811-020-02190-5
Fernandez-Valenzuela, S., Chávez-Ruvalcaba, F., Beltran-Rocha, J. C., San Claudio, P. M., & Reyna-Martínez, R. (2021). Isolation and Culturing Axenic Microalgae: Mini–Review. The Open Microbiology Journal, 15(1), 111-119. https://doi.org/10.2174/1874285802115010111
Gross, O. P. W. (2004). Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol, 65, 635-648. https://doi.org/10.1007/s00253-004-1647-x
Janse van Vuuren, S. (2006). Easy identification of the most common freshwater algae. A guide for the identification of microscopic algae in South African freshwaters.
Maltsev, Y. I., & Konovalenko, T. V. (2017). New finding of green algae with potential for algal biotechnology, Chlorococcum oleofaciens and its molecular investigation. Regulatory Mechanisms in Biosystems, 8(4), 532-539. https://doi.org/10.15421/021782
Nucleotide BLAST: Search nucleotide databases using a nucleotide query. (n.d.). Retrieved May 1, 2024, from https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome
Pham, T. L. (2017). Environmental gradients regulate the spatio-temporal variability of phytoplankton assemblages in the Can Gio Mangrove Biosphere Reserve, Vietnam. Ocean Science Journal, 52(4), 537-547. https://doi.org/10.1007/s12601-017-0045-0
Scenedesmus. (n.d.). Retrieved May 29, 2024, from https://fmp.conncoll.edu/Silicasecchidisk/LucidKeys3.5/Keys_v3.5/Carolina35_Key/Media/Html/Scenedesmus_Ecology.html