TWO NEW STRAINS OF MICROALGAE Scenedesmus sp. WERE RECENTLY ISOLATED AND IDENTIFIED BASED ON THE 18S SEQUENCE FROM THE CAN GIO MANGROVE BIOSPHERE RESERVE

Abstract

The microalgae Scenedesmus belongs to the Chlorophyta phylum and is a widely distributed freshwater algae species. Many species of microalgae from the genus Scenedesmus are found in the Can Gio mangrove biosphere reserve and play a significant role in the water environment. In this study, two microalgae strains, Scenedesmus CG01 and CG03, were newly isolated from two different sampling locations in the Can Gio mangrove biosphere reserve. These two strains have similar morphological characteristics and share typical features of the genus Scenedesmus. Through PCR, sequencing, and identification based on the 18S DNA sequence, it was confirmed that these two strains, CG01 and CG03, are Scenedesmus sp. The 18S DNA sequences of these two strains have some differences compared to the sequences of Scenedesmus sp. CCAP 217/7 and CCAP217/8 in GenBank. Based on the 18S sequences, the phylogenetic tree also shows that CG01 and CG03 strains are in a group, separate from the remaining samples. This research on the isolation and accurate identification of species provides an important foundation for future studies on the application of microalgae.