THE PROTECTIVE EFFECT OF ACEROLA FRUIT JUICE AGAINST LEAD TOXICITY ON RED BLOOD CELL SIZE IN MALE ALBINO MICE
Nội dung chính của bài viết
Tóm tắt
This study evaluated the protective effects of acerola fruit juice against lead toxicity by examining the size of red blood cells in male albino mice. The mice were divided into 6 groups, including a control group, a lead-exposed group (70 mg/kg body weight), a vitamin C group (30 mg/kg body weight), and groups exposed to lead in combination with acerola fruit juice at doses of 20, 30, and 40 mg/kg body weight, respectively. The results revealed that while lead exposure over four and eight weeks led to smaller mouse red blood cells, concurrent administration of acerola fruit juice, especially at 40 mg/kg, effectively maintained normal red blood cell size, counteracting lead's toxic effects. The present study suggests the potential protective effect of acerola fruit juice against lead-induced toxicity, and recommends further investigation to elucidate the underlying mechanism and explore its potential uses.
Từ khóa
acerola fruit juice, albino mice, lead toxicity, red blood cell size, vitamin C.
Chi tiết bài viết
Tài liệu tham khảo
REFERENCES
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Andjelkovic, M., Buha Djordjevic, A., Antonijevic, E., Antonijevic, B., Stanic, M., Kotur-Stevuljevic, J., . . . Bulat, Z. (2019). Toxic Effect of Acute Cadmium and Lead Exposure in Rat Blood, Liver, and Kidney. Int J Environ Res Public Health, 16(2). doi:10.3390/ijerph16020274
Autifi, M. A. H., Mohamed, W. Y., Abdul Haye, W. M., & Elbaz, K. R. (2018). The possible protective role of Vitamin C against toxicity induced by lead acetate in liver and spleen of adult albino rats (Light and Electron Microscopic Study). The Egyptian Journal of Hospital Medicine, 73(10), 7650-7658.
Balali-Mood, M., Naseri, K., Tahergorabi, Z., Khazdair, M. R., & Sadeghi, M. (2021). Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic. Front Pharmacol, 12, 643972. doi:10.3389/fphar.2021.643972
Bergeson, L. L. J. E. Q. M. (2008). The proposed lead NAAQS: Is consideration of cost in the clean air act's future? , 18(1).
Bourekoua, H., Gawlik-Dziki, U., Różyło, R., Zidoune, M. N., & Dziki, D. (2021). Acerola fruit as a natural antioxidant ingredient for gluten-free bread: An approach to improve bread quality. Food science and technology international, 27(1), 13-21.
Bowen-Forbes, C. S., Zhang, Y., Nair, M. G. J. J. o. f. c., & analysis. (2010). Anthocyanin content, antioxidant, anti-inflammatory and anticancer properties of blackberry and raspberry fruits. 23(6), 554-560. doi:10.1016/j.jfca.2009.08.012
Christine A. Moore, & Adil, A. (2022). Macrocytic Anemia. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK459295/ (truy cập 31-01-2025)
Ekanem, A., Kwari, H., Garba, S., & Salami, H. (2015). Effect of lead acetate on spleen and blood parameters in albino rats. IOSR J. Dent. Med. Sci. Ver. I, 14, 43-49. doi:10.9790/0853-14314349
El-Tantawy, W. H. (2016). Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. J Tradit Complement Med, 6(4), 327-331. doi:10.1016/j.jtcme.2015.02.001
Garcia, V. A. d. S., Borges, J. G., Vanin, F. M., & Carvalho, R. A. d. (2020). Vitamin C stability in acerola and camu-camu powder obtained by spray drying. Brazilian Journal of Food Technology, 23, e2019237. doi:10.1590/1981-6723.23719
Hammad S. Chaudhry, M. R. K. (2023). Microcytic Hypochromic Anemia. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK470252/ (truy cập ngày 31-01-2025)
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Ibrahim, N. M., Eweis, E. A., El-Beltagi, H. S., & Abdel-Mobdy, Y. E. (2012). Effect of lead acetate toxicity on experimental male albino rat. Asian Pacific journal of tropical biomedicine, 2(1), 41-46. doi:10.1016/S2221-1691(11)60187-1
Mani, M. S., Joshi, M. B., Shetty, R. R., VL, D. S., Swathi, M., Kabekkodu, S. P., & Dsouza, H. S. (2020). Lead exposure induces metabolic reprogramming in rat models. Toxicol Lett, 335, 11-27. doi:10.1016/j.toxlet.2020.09.010
Mohanty, J. G., Nagababu, E., & Rifkind, J. M. (2014). Red blood cell oxidative stress impairs oxygen delivery and induces red blood cell aging. Front Physiol, 5, 84. doi:10.3389/fphys.2014.00084
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Nguyen, T. T. H., & Vo, V. T. (2019). Experiment of Human and Animal physiolgy (text in Vietnamese): Ho Chi Minh City University of Education Publishing House
Notariale, R., Infantino, R., Palazzo, E., & Manna, C. (2021). Erythrocytes as a Model for Heavy Metal-Related Vascular Dysfunction: The Protective Effect of Dietary Components. Int J Mol Sci, 22(12). doi:10.3390/ijms22126604
Ojeka, S., Ukoro, B., Onwoke, E. E. J. I. B. R., & Reviews. (2024). Antioxidant Effects of Vitamin C on Some Hematological Parameters of Male Wistar Rats Exposed to Lead Acetate. International Blood Research & Reviews, 15(2), 10-21. doi:10.9734/ibrr/2024/v15i2335
Pehlivan, F. E. (2017). Vitamin C: An antioxidant agent. Vitamin C, 2, 23-35. doi:10.5772/intechopen.69660
Peters, J. L., Perry, M. J., McNeely, E., Wright, R. O., Heiger-Bernays, W., & Weuve, J. (2021). The association of cadmium and lead exposures with red cell distribution width. PLoS ONE, 16(1), e0245173. doi:10.1371/journal.pone.0245173
Prakash, A., & Baskaran, R. (2018). Acerola, an untapped functional superfruit: a review on latest frontiers. J Food Sci Technol, 55(9), 3373-3384. doi:10.1007/s13197-018-3309-5
Sani, A. H., & Amanabo, M. (2021). Lead: A concise review of its toxicity, mechanism and health effect. GSC Biological and Pharmaceutical Sciences, 15(1), 055-062. doi:10.30574/gscbps.2021.15.1.0096
Sharaf, A., Farrag, A., & Fahmy, H. (2017). Protective effects of vitamin C on hematological and biochemical parameters of intoxicated male albino rats with lead and cadmium. Middle East J. Appl. Sci, 7, 57-67.
The Natural Food Hub. (2018). Natural food Fruit Vitamin C Content. http://www.naturalhub.com/natural_food_guide_fruit_vitamin_c.htm., Accessed at 10:00 am, 16/01/2018. Retrieved from http://www.naturalhub.com/natural_food_guide_fruit_vitamin_c.htm., Accessed at 10:00 am, 16/01/2018.
Thuong, H. N. T., Lan, C. T. N., Van, T. T., Thanh, H. N. T., & Ke, Q. T. (2023). Effect of lead on hematological parameters and histopathology of the liver, kidney and spleen of female albino mice. Asian Journal of Health Sciences, 9(1), ID52-ID52. doi:10.15419/ajhs.v9i1.523
Vilvert, J. C., de Freitas, S. T., dos Santos, L. F., S. Ribeiro, T. d., Veloso, C. M. J. J. o. F. M., & Characterization. (2024). Phenolic compounds in acerola fruit and by-products: An overview on identification, quantification, influencing factors, and biological properties. 18(1), 216-239. doi:10.1007/s11694-023-02175-1
World Health Organization. (2024). Lead poisoning. Retrieved from https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health (truy cập 31-01-2025)
Ziamajidi, N., Daei, S., Khajvand-Abedini, M., Abbasalipourkabir, R., & Nourian, A. (2023). Vitamins A, C, and E Exert Anti-apoptotic Function in the Testis of Rats After Exposure to Zinc Oxide Nanoparticles. Chonnam Med J, 59(1), 48-53. doi:10.4068/cmj.2023.59.1.48
Abou-Zeid, S. M., Allam, T., El-Bahrawy, A., & Mohamed, A. (2018). Ameliorating effects of green tea on ethephon-induced immunotoxicity and oxidative stress in mice. Int J Pharm Sci Sci Res, 4(1), 1-9. doi:10.25141/2471-6782-2018-1.0001
Andjelkovic, M., Buha Djordjevic, A., Antonijevic, E., Antonijevic, B., Stanic, M., Kotur-Stevuljevic, J., . . . Bulat, Z. (2019). Toxic Effect of Acute Cadmium and Lead Exposure in Rat Blood, Liver, and Kidney. Int J Environ Res Public Health, 16(2). doi:10.3390/ijerph16020274
Autifi, M. A. H., Mohamed, W. Y., Abdul Haye, W. M., & Elbaz, K. R. (2018). The possible protective role of Vitamin C against toxicity induced by lead acetate in liver and spleen of adult albino rats (Light and Electron Microscopic Study). The Egyptian Journal of Hospital Medicine, 73(10), 7650-7658.
Balali-Mood, M., Naseri, K., Tahergorabi, Z., Khazdair, M. R., & Sadeghi, M. (2021). Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic. Front Pharmacol, 12, 643972. doi:10.3389/fphar.2021.643972
Bergeson, L. L. J. E. Q. M. (2008). The proposed lead NAAQS: Is consideration of cost in the clean air act's future? , 18(1).
Bourekoua, H., Gawlik-Dziki, U., Różyło, R., Zidoune, M. N., & Dziki, D. (2021). Acerola fruit as a natural antioxidant ingredient for gluten-free bread: An approach to improve bread quality. Food science and technology international, 27(1), 13-21.
Bowen-Forbes, C. S., Zhang, Y., Nair, M. G. J. J. o. f. c., & analysis. (2010). Anthocyanin content, antioxidant, anti-inflammatory and anticancer properties of blackberry and raspberry fruits. 23(6), 554-560. doi:10.1016/j.jfca.2009.08.012
Christine A. Moore, & Adil, A. (2022). Macrocytic Anemia. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK459295/ (truy cập 31-01-2025)
Ekanem, A., Kwari, H., Garba, S., & Salami, H. (2015). Effect of lead acetate on spleen and blood parameters in albino rats. IOSR J. Dent. Med. Sci. Ver. I, 14, 43-49. doi:10.9790/0853-14314349
El-Tantawy, W. H. (2016). Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. J Tradit Complement Med, 6(4), 327-331. doi:10.1016/j.jtcme.2015.02.001
Garcia, V. A. d. S., Borges, J. G., Vanin, F. M., & Carvalho, R. A. d. (2020). Vitamin C stability in acerola and camu-camu powder obtained by spray drying. Brazilian Journal of Food Technology, 23, e2019237. doi:10.1590/1981-6723.23719
Hammad S. Chaudhry, M. R. K. (2023). Microcytic Hypochromic Anemia. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK470252/ (truy cập ngày 31-01-2025)
Healthline. (2019). Everything You Need to Know About Microcytic Anemia. Retrieved from https://www.healthline.com/health/microcytic-anemia (truy cập ngày 31-1-2025)
Ibrahim, N. M., Eweis, E. A., El-Beltagi, H. S., & Abdel-Mobdy, Y. E. (2012). Effect of lead acetate toxicity on experimental male albino rat. Asian Pacific journal of tropical biomedicine, 2(1), 41-46. doi:10.1016/S2221-1691(11)60187-1
Mani, M. S., Joshi, M. B., Shetty, R. R., VL, D. S., Swathi, M., Kabekkodu, S. P., & Dsouza, H. S. (2020). Lead exposure induces metabolic reprogramming in rat models. Toxicol Lett, 335, 11-27. doi:10.1016/j.toxlet.2020.09.010
Mohanty, J. G., Nagababu, E., & Rifkind, J. M. (2014). Red blood cell oxidative stress impairs oxygen delivery and induces red blood cell aging. Front Physiol, 5, 84. doi:10.3389/fphys.2014.00084
Moran, U. (2001-2005). Red blood cell diameter - Mouse Mus musculus. In Red blood cell diameter, Cell Size Database: T. Ryan Gregory.
National_Institutes_of_Health. (2021). Vitamin C. Retrieved from https://ods.od.nih.gov/factsheets/VitaminC-HealthProfessional/
NatureClaim. (2024, 2024-09-04). Acerola Nutrition. Retrieved from https://natureclaim.com/nutrition/info/acerola/ (truy cập ngày 31-01-2025)
Nguyen, T. T. H., & Vo, V. T. (2019). Experiment of Human and Animal physiolgy (text in Vietnamese): Ho Chi Minh City University of Education Publishing House
Notariale, R., Infantino, R., Palazzo, E., & Manna, C. (2021). Erythrocytes as a Model for Heavy Metal-Related Vascular Dysfunction: The Protective Effect of Dietary Components. Int J Mol Sci, 22(12). doi:10.3390/ijms22126604
Ojeka, S., Ukoro, B., Onwoke, E. E. J. I. B. R., & Reviews. (2024). Antioxidant Effects of Vitamin C on Some Hematological Parameters of Male Wistar Rats Exposed to Lead Acetate. International Blood Research & Reviews, 15(2), 10-21. doi:10.9734/ibrr/2024/v15i2335
Pehlivan, F. E. (2017). Vitamin C: An antioxidant agent. Vitamin C, 2, 23-35. doi:10.5772/intechopen.69660
Peters, J. L., Perry, M. J., McNeely, E., Wright, R. O., Heiger-Bernays, W., & Weuve, J. (2021). The association of cadmium and lead exposures with red cell distribution width. PLoS ONE, 16(1), e0245173. doi:10.1371/journal.pone.0245173
Prakash, A., & Baskaran, R. (2018). Acerola, an untapped functional superfruit: a review on latest frontiers. J Food Sci Technol, 55(9), 3373-3384. doi:10.1007/s13197-018-3309-5
Sani, A. H., & Amanabo, M. (2021). Lead: A concise review of its toxicity, mechanism and health effect. GSC Biological and Pharmaceutical Sciences, 15(1), 055-062. doi:10.30574/gscbps.2021.15.1.0096
Sharaf, A., Farrag, A., & Fahmy, H. (2017). Protective effects of vitamin C on hematological and biochemical parameters of intoxicated male albino rats with lead and cadmium. Middle East J. Appl. Sci, 7, 57-67.
The Natural Food Hub. (2018). Natural food Fruit Vitamin C Content. http://www.naturalhub.com/natural_food_guide_fruit_vitamin_c.htm., Accessed at 10:00 am, 16/01/2018. Retrieved from http://www.naturalhub.com/natural_food_guide_fruit_vitamin_c.htm., Accessed at 10:00 am, 16/01/2018.
Thuong, H. N. T., Lan, C. T. N., Van, T. T., Thanh, H. N. T., & Ke, Q. T. (2023). Effect of lead on hematological parameters and histopathology of the liver, kidney and spleen of female albino mice. Asian Journal of Health Sciences, 9(1), ID52-ID52. doi:10.15419/ajhs.v9i1.523
Vilvert, J. C., de Freitas, S. T., dos Santos, L. F., S. Ribeiro, T. d., Veloso, C. M. J. J. o. F. M., & Characterization. (2024). Phenolic compounds in acerola fruit and by-products: An overview on identification, quantification, influencing factors, and biological properties. 18(1), 216-239. doi:10.1007/s11694-023-02175-1
World Health Organization. (2024). Lead poisoning. Retrieved from https://www.who.int/news-room/fact-sheets/detail/lead-poisoning-and-health (truy cập 31-01-2025)
Ziamajidi, N., Daei, S., Khajvand-Abedini, M., Abbasalipourkabir, R., & Nourian, A. (2023). Vitamins A, C, and E Exert Anti-apoptotic Function in the Testis of Rats After Exposure to Zinc Oxide Nanoparticles. Chonnam Med J, 59(1), 48-53. doi:10.4068/cmj.2023.59.1.48